Denitrification Characteristics of Aerobic Denitrifying Bacteria Achromobacter sp. L16

doi:10.13560/j.cnki.biotech.bull.1985.2019-0978

Abstract

Abstract: A heterotrophic nitrification-aerobic denitrification strain L16 was isolated from landfill leachate and identified as Achromobacter sp. by morphological observation and 16s rDNA gene sequence analysis. The rate of removing ammonia nitrogen was 61.94% and that of removing nitrate nitrogen was 98.40%. The culture conditions of aerobic denitrification and heterotrophic denitrification of strain L16 was optimized by single factor experiment. The optimized results of aerobic denitrification and heterotrophic nitrification of strain L16 showed that L16 was cultured with nitrate as nitrogen source,sodium citrate as carbon source,C/N 20,culture temperature 30℃,rotate speed 150 r/min,and the degradation rate of nitrate nitrogen and the rate of removing total nitrogen was 99.74% and 58.90%, respectively. Under the conditions of ammonia nitrogen as nitrogen source,sodium citrate as carbon source,C /N 20,culture temperature 30℃ and rotate speed 200 r/min,and the degradation rate of ammonia nitrogen increased to 93.41% and the removal rate of total nitrogen was 86.33%. The optimized strain L16 has high efficiency of heterotrophic nitrification-aerobic denitrification,converts the most of the nitrogen into gas and intracellular nitrogen,and thus has potential value of practical application in wastewater treatment.

Key words: erobic denitrification, heterotrophic nitrification, Achromobacter sp.

LI Si-qi, YANG Jing-dan, LIU Lin, LIU Er-jia, WANG Xiao-hui. Denitrification Characteristics of Aerobic Denitrifying Bacteria Achromobacter sp. L16[J]. Biotechnology Bulletin, 2020, 36(6): 93-101.

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