Changes in Plasma Metabolites After Calving in Dairy Cows

doi:10.13560/j.cnki.biotech.bull.1985.2019-1065

Abstract

Abstract: This work aims to study the changes of plasma metabolites in dairy cows in the calving and postpartum period. Fifteen healthy and high-yield dairy cows with similar parity,expected date of birth and similar body conditions were selected for collecting 10 mL blood from the tail vein on the day of calving and on the 7^th day after calving when before morning feeding. The blood plasma of the cows was analyzed by ultra-high performance liquid chromatography-quadrupole-time-of-fight-mass spectrometry（UHPLC-QTOF-MS）. Combined with principal component analysis（PCA）and orthogonal partial least squares-discriminant analysis（OPLS-DA）,plasma differential metabolites between 2 groups were screened based on the P<0.05,VIP>1 and FC>1.3 or FC<0.77. The ROC curve further was used to study the ability of these significant differential metabolites to be classified and identified. The results showed that 11 significant up-regulated differential metabolites were found by ROC curve：PC（16：0/16：0）,glycolithocholic acid,pro-ser,pro-gly,L-stearoyl-sn-glycerol 3-phosphocholine,orotic acid,L-aspartate,N-acetyl-L-aspartic acid,isovalerylglycine,L-calving-carnitine and benzoic acid. There were significant differences in the metabolic profiles of alanine,aspartic acid and glutamic acid metabolism,glycerophospholipid metabolism and pyrimidine metabolism between the day of calving and on the 7^th day after calving. In summary,the changes of N-acetyl-L-aspartic acid,L-aspartic acid,PC（16：0/16：0）and orotic acid are beneficial to maintain the health of cows after postpartum period.

Key words: postpartum dairy cows, calving, metabolite

ZHANG Meng, LUO Fang, WANG Min, WU Yan-ze, WANG Jun-kui, HE Dong-qian, CHEN Li-yao, TAO Jin-zhong. Changes in Plasma Metabolites After Calving in Dairy Cows[J]. Biotechnology Bulletin, 2020, 36(6): 191-199.

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