Effects of Crop Rotation on Bacterial Communities in Cotton Rhizosphere Soil

doi:10.13560/j.cnki.biotech.bull.1985.2020-0685

Abstract

Abstract: In order to analyze the effect of crop rotation on bacterial diversity and community structure in the rhizosphere soil of cotton,cotton was used as the research object,peanut and millet as rotation crop materials. IonS5^TMXL high-throughput sequencing platform was used to sequence the 16S rDNA of each treated soil sample. The results showed that the crop rotation increased the yield of cotton,by 32.19% with peanut-cotton rotation and 18.13% with millet-cotton,respectively. Though rotation was not sufficient to completely alter the bacterial community structure in the rhizosphere soil of cotton,it led to a significant increase in the relative abundances of Bacteroidetes,Proteobacteria,Planctomycetes,and Sphingomonas in the flora. Enrichment of these beneficial phylum or genera inhibited the activity of pathogens and led to a decrease in bacterial community richness. Rotation planting may affect soil bacteria to improve quality of cotton life,and then increase cotton yield.

Key words: cotton, crop rotation, high-throughput sequencing, soil bacteria, community structure

WANG Hong-jie, LIU Shao-dong, LIU Rui-hua, ZHANG Si-ping, YANG Jun, PANG Chao-you. Effects of Crop Rotation on Bacterial Communities in Cotton Rhizosphere Soil[J]. Biotechnology Bulletin, 2020, 36(9): 117-124.

References 29

[1]	林先贵, 胡君利. 土壤微生物多样性的科学内涵及其生态服务功能[J]. 土壤学报, 2008, 45(5):892-900.Lin XG, Hu JL.Scientific connotation and ecological service function of soil microbial diversity[J]. Acta Pedologica Sinica, 2008, 45(5):892-900.
[2]	薛超, 黄启为, 凌宁, 等. 连作土壤微生物区系分析、调控及高通量研究方法[J]. 土壤学报, 2011, 48(3):612-618.Xue C, Huang QW, Ling N, et al.Analysis, regulation and high-throughput sequencing of soil microflora in mono-cropping system[J]. Acta Pedologica Sinica, 2011, 48(3):612-618.
[3]	刘军, 唐志敏, 刘建国, 等. 长期连作及秸秆还田对棉田土壤微生物量及种群结构的影响[J]. 生态环境学报, 2012, 21(8):1418-1422.Liu J, Tang ZM, Liu JG, et al.Effects of cotton continuous cropping and returning stalks to soil on the quantities and community structure of soil microbes[J]. Ecology and Environmental Sciences, 2012, 21(8):1418-1422.
[4]	张晓玲, 潘振刚, 周晓锋, 等. 自毒作用与连作障碍[J]. 土壤通报, 2007(4):781-784.Zhang XL, Pan ZG, Zhou XF, et al.Autotoxicity and continuous cropping obstacles:A review[J]. Chinese Journal of Soil Science, 2007(4):781-784.
[5]	郑良永, 胡剑非, 林昌华, 等. 作物连作障碍的产生及防治[J]. 热带农业科学, 2005(2):58-62.Zheng LY, Hu JF, Lin CH, et al.The production of succession cropping obstacles and its prevention and cure steps[J]. Chinese Journal of Tropical Agriculture, 2005(2):58-62.
[6]	刘金波, 许艳丽. 我国连作大豆土壤微生物研究现状[J]. 中国油料作物学报, 2008(1):132-136.Liu JB, Xu YL.Current research of soil microbial of successive soybean cropping in China[J]. Chinese Journal of Oil Crop Science, 2008(1):132-136.
[7]	李文娇, 杨殿林, 赵建宁, 等. 长期连作和轮作对农田土壤生物学特性的影响研究进展[J]. 中国农学通报, 2015(3):173-178.Li WJ, Yang DL, Zhao JN, et al.Progress on effects of long-term continuous cropping and rotation systems on farmland soil biological characteristics in farmland[J]. Chinese Agricultural Science Bulletin, 2015(3):173-178.
[8]	祁虹, 王树林, 王燕, 等. 粮棉轮作与土壤深翻对棉花生育性状及产量的影响[J]. 天津农业科学, 2016, 22(8):113-116.Qi H, Wang SL, Wang Y, et al.Effects of rotation and soil deep ploughing on cotton development traits and yield[J]. Tianjin Agricultural Sciences, 2016, 22(8):113-116.
[9]	姚钦. 黑土区轮作方式下土壤微生物多样性研究[D]. 哈尔滨:东北林业大学, 2012.Yao Q.Microorganism diversity of mollisols in different rotation systems[D]. Harbin:Northeast Forestry University, 2012.
[10]	John D, Barns SM, Ticknor LO, et al.Empirical and theoretical bacterial diversity in four Arizona soils[J]. Applied and Environmental Microbiology, 2002, 68(6):3035-3045.
[11]	蒋永梅, 师尚礼, 田永亮, 等. 高寒草地不同退化程度下土壤微生物及土壤酶活性变化特征[J]. 水土保持学报, 2017, 31(3):244-249.Jiang YM, Shi SL, Tian YL, et al.Characteristics of soil microorganism and soil enzyme activities in alpine meadows under different degrees of degradation[J]. Journal of Soil and Water Conservation, 2017, 31(3):244-249.
[12]	Delgado-Baquerizo M, Maestre FT, Reich PB, et al.Carbon content and climate variability drive global soil bacterial diversity patterns[J]. Ecological Monographs, 2016, 86(3):373-390.
[13]	吕雅悠, 于迪, 丁方丽, 等. 促植物生长根际细菌A21-4对田间辣椒生长及根际土壤微生态环境的影响[J]. 中国生物防治学报, 2016, 32(1):86-92.Lu YY, Yu D, Ding FL, et al.Effects of PGPR strain A21-4 on growth and rhizosphere soil charactors of pepper in field[J]. Chinese Journal of Biological Control, 2016, 32(1):86-92.
[14]	王娜. 植物内生细菌的定殖动态及其对辣椒疫病和棉花黄萎病的防治效果研究[D]. 南京:南京师范大学, 2015.Wang N.Colonization dynamics of endophytic bacteria and its effect on control of pepper blight and cotton verticillium wilt[D]. Nanjing:Nanjing Normal University, 2015.
[15]	Schuster SC.Next-generation sequencing transforms today’s biology[J]. Nature Methods, 2008, 5(1):16-18.
[16]	吴艳飞, 张雪艳, 李元, 等. 轮作对黄瓜连作土壤环境和产量的影响[J]. 园艺学报, 2008(3):357-362.Wu YF, Zhang XY, Li Y, et al.Influence of rotation on continuous cropping soil environment and cucumber yield[J]. Acta Horticulturae Sinica, 2008(3):357-362.
[17]	Stark C, Condron LM, Stewart A, et al.Effects of past and current crop management on soil microbial biomass and cativity[J]. Biology and Fertility of Soils, 2007, 43(5):531-540.
[18]	徐文修, 罗明, 李银平, 等. 作物茬口对连作棉田土壤环境及棉花产量的影响[J]. 农业工程学报, 2011, 27(3):271-275.Xu WX, Luo M, Li YP, et al.Effects of crop stubbles on cotton yield and soil environment in continuously cropped cotton field[J]. Transactions of the Chinese Society of Agricultural Engineering, 2011, 27(3):271-275.
[19]	Coskun D, Britto DT, Shi W, et al.How plant root exudates shape the nitrogen cycle[J]. Trends Plant Sci, 2017, 22:661-673.
[20]	吴凤芝, 赵凤艳, 刘元英. 设施蔬菜连作障碍原因综合分析与防治措施[J]. 东北农业大学学报, 2000(3):241-247.Wu FZ, Zhao FY, Liu YY.On the reasons of continuous cropping obstacles in vegetable facility gardening[J]. Journal of Northeast Agricultural University, 2000(3):241-247.
[21]	高旭梅, 刘娟, 张前兵, 等. 耕作措施对新疆绿洲长期连作棉田土壤微生物、酶活性的影响[J]. 石河子大学学报:自然科学版, 2011, 29(2):145-152.Gao XM, Liu J, Zhang QB, et al.Effects of tillage practices on soil microbial and enzyme activity in long-term continuous cotton of xinjiang oasis[J]. Journal of Shihezi University:Natural Science Edition, 2011, 29(2):145-152.
[22]	徐瑞富, 王小龙. 花生连作田土壤微生物群落动态与土壤养分关系研究[J]. 花生学报, 2003(3):19-24.Xu RF, Wang XL.Relation of microbial population dynamics and nutrient in soil of continuous cropping with peanut[J]. Journal of Peanut Science, 2003(3):19-24.
[23]	苗祯, 杜宗军, 李会荣, 等. 5株北极微藻藻际环境的细菌多样性[J]. 生态学报, 2015, 35(5):1587-1600.Miao Z, Du ZJ, Li HR, et al.Analysis of bacterial diversity in the phycosphere of five Arctic microalgae[J]. Acta Ecologica Sinica, 2015, 35(5):1587-1600.
[24]	王悦, 杨贝贝, 王浩, 等. 不同种植模式下丹参根际土壤微生物群落结构变化[J]. 生态学报, 2019, 39(13):4832-4843.Wang Y, Yang BB, Wang H, et al.Variation in microbial community structure in the rhizosphere soil of Salvia miltiorrhiza Bunge under three cropping modes[J]. Acta Ecologica Sinica, 2019, 39(13):4832-4843.
[25]	孙建光, 张燕春, 徐晶, 等. 玉米根际高效固氮菌Sphingomonas sp. GD542的分离鉴定及接种效果初步研究[J]. 中国生态农业学报, 2010, 18(1):89-93.Sun JG, Zhang YC, Xu J, et al.Isolation, identification and inoculation effect of nitrogen-fixing bacteria Sphingomonas GD542 from maize rhizosphere[J]. Chinese Journal of Eco-Agriculture, 2010, 18(1):89-93.
[26]	王秀红, 李欣欣, 史向远, 等. 好氧堆肥微生物代谢多样性及其细菌群落结构[J]. 环境科学研究, 2018, 31(8):1457-1463.Wang XH, Li XX, Shi XY, et al.Microbial metabolism diversity and bacterial flora structure during aerobic composting[J]. Research of Environmental Sciences, 2008, 31(8):1457-1463.
[27]	Jolliffe IT.Principal component analysis[J]. Journal of Marketing Research, 1986, 87(100):513.
[28]	Li Y, Xu L.An improvement for unweighted pair group method with arithmetic mean and its application[J]. Journal of Beijing University of Technology, 2007, 33(12):1333-1339.
[29]	Li XG, Ding CF, Hua K, et al.Soil sickness of peanuts is attributable to modifications in soil microbes induced by peanut root exudates rather than to direct allelopathy[J]. Soil Biology and Biochemistry, 2014, 78:148-159.