Characteristics of Soil Bacterial Community Structure in the Different Developmental Stages of Desert Grassland Caragana korshinskii Kom. Nebkhas

doi:10.13560/j.cnki.biotech.bull.1985.2022-0248

Abstract

Abstract:

To understand the soil bacterial community structure and diversity characteristics and the influencing factors at different developmental stages of Caragana korshinskii Kom. nebkhas in arid and semi-arid desert grasslands,C. korshinskii Kom. nebkhas in Ningxia desert grasslands at three different developmental stages of growing,mature,and recession were selected. At the same time,high-throughput sequencing technology was applied to analyze their soil bacterial community structure and diversity characteristics,and to investigate the influencing factors of developmental stages and soil physicochemical factors evolution on soil bacterial community structure and function. Finally,PICRUSt method was to predict the functions of soil bacteria at different developmental stages of C. korshinskii Kom. nebkhas. The results showed that the obtained 15 383 operational taxonomic units（OTUs）belonged to 50 phyla,146 classes,364 orders,590 families,and 1 111 genera,with the largest number of bacteria at the growing stage and the dominant phyla being 9 of Actinobacteriota,Proteobacteria,Acidobacteriota,Chloroflexi,Crenarchaeota,and nine others. Proteobacteria,Acidobacteriota,Chloroflexi,and others,accounting for > 90% of the total number of microorganisms,and the dominant phyla were similar to most studies. There were significant differences in soil AP and EC in different development stages of C. korshinskii Kom. nebkhas,showing mature> growing > recession. Other soil nutrients were not significantly different,but the values were also expressed as mature> growing > recession. There was no significant difference in the diversity and abundance of bacterial communities,but the numerical value was the highest in the mature period. AP and SOC were the main factors influencing the diversity of soil bacterial communities in C. korshinskiiKom. nebkhas. Other environmental factors such as EC and pH also significantly affected the richness of multiple bacterial communities. Based on the prediction of 16S rRNA sequences of soil bacterial genomes in different developmental stages of C. korshinskii Kom. nebkhas,it was found that metabolism was the main function of soil bacteria. The above results can enrich the theoretical knowledge of soil microbial diversity in semi-arid desert grasslands and provide a reference for the restoration of C. korshinskii Kom. vegetation.

Key words: desert steppe, Caragana korshinskii Kom., nebkhas, soil bacteria, community structure, functional prediction

WANG Zi-yin, LIU Bing-ru, LI Zi-hao, ZHAO Xiao-yu. Characteristics of Soil Bacterial Community Structure in the Different Developmental Stages of Desert Grassland Caragana korshinskii Kom. Nebkhas[J]. Biotechnology Bulletin, 2022, 38(7): 205-214.

Figures/Tables 8

References 34

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发育阶段 Developmental stage	有机碳SOC/（g·kg^-1）	总磷TP/（g·kg^-1）	速效磷AP/（mg·kg^-1）	总氮TN/（g·kg^-1）	电导率EC/（ms·cm^-1）	pH
生长期Growing	3.080±0.534a	0.238±0.020a	3.124±0.240b	0.156±0.005a	74.832±7.615ab	7.638±0.029a
成熟期Mature	3.244±0.623a	0.245±0.016a	3.508±0.221a	0.168±0.019a	81.134±6.779a	7.636±0.062a
衰退期Recession	2.646±0.370a	0.232±0.012a	2.924±0.228b	0.148±0.023a	69.434±9.328b	7.656±0.030a

发育阶段 Developmental stage	有机碳SOC/（g·kg^-1）	总磷TP/（g·kg^-1）	速效磷AP/（mg·kg^-1）	总氮TN/（g·kg^-1）	电导率EC/（ms·cm^-1）	pH
生长期Growing	3.080±0.534a	0.238±0.020a	3.124±0.240b	0.156±0.005a	74.832±7.615ab	7.638±0.029a
成熟期Mature	3.244±0.623a	0.245±0.016a	3.508±0.221a	0.168±0.019a	81.134±6.779a	7.636±0.062a
衰退期Recession	2.646±0.370a	0.232±0.012a	2.924±0.228b	0.148±0.023a	69.434±9.328b	7.656±0.030a

发育阶段 Developmental stage	序列数 Sequence number	Shannon指数 Shannon index	ACE指数 ACE index	Chao 1指数 Chao 1 index	覆盖度 Coverage/%
生长期Growing	69731±1774a	6.828±0.024a	5746.43±318.25a	5413.55±168.93a	95.66±0.17a
成熟期Mature	62230±4634b	6.853±0.066a	5810.29±472.11a	5572.59±297.17a	95.52±0.27a
衰退期Recession	56050±3653c	6.852±0.030a	5736.14±296.62a	5435.89±163.83a	95.65±0.14a

发育阶段 Developmental stage	序列数 Sequence number	Shannon指数 Shannon index	ACE指数 ACE index	Chao 1指数 Chao 1 index	覆盖度 Coverage/%
生长期Growing	69731±1774a	6.828±0.024a	5746.43±318.25a	5413.55±168.93a	95.66±0.17a
成熟期Mature	62230±4634b	6.853±0.066a	5810.29±472.11a	5572.59±297.17a	95.52±0.27a
衰退期Recession	56050±3653c	6.852±0.030a	5736.14±296.62a	5435.89±163.83a	95.65±0.14a

一级通路 Pathway level 1	二级通路Pathway level 2	生长期 Growing	占比 Percentage/%	成熟期 Mature	占比 Percentage/%	衰退期Recession	占比 Percentage/%
代谢Metabolism	全局概况图Global and overview maps	35234604	40.64	35808222	40.61	35369151	40.70
	碳水化合物代谢Carbohydrate metabolism	7961526.76	9.18	8095202.2	9.18	8011233	9.22
	氨基酸代谢Amino acid metabolism	7180071.5	8.28	7313111.6	8.29	7204390	8.29
	能量代谢Energy metabolism	3897443.61	4.50	3946546.9	4.48	3902439	4.49
	辅酶和维生素代谢Metabolism of cofactors and vitamins	3698076.74	4.27	3754449.3	4.26	3702735	4.26
	核苷酸代谢Nucleotide metabolism	2042528.25	2.36	2069459.1	2.35	2045558	2.35
	脂质代谢Lipid metabolism	1881022.7	2.17	1931092.1	2.19	1894049	2.18
	外源生物降解与代谢Xenobiotics biodegradation and metabolism	1731831.36	2.00	1800961.4	2.04	1737606	2.00
	其他次生代谢产物的生物合成Biosynthesis of other secondary metabolites	1366994.13	1.58	1385902.6	1.57	1372414	1.58
	其他氨基酸代谢Metabolism of other amino acids	1312324.37	1.51	1349247.2	1.53	1317989	1.52
	萜类化合物和聚酮类化合物代谢Metabolism of terpenoids and polyketides	987922.99	1.14	1005755.7	1.14	993177	1.14
	聚糖生物合成和代谢Glycan biosynthesis and metabolism	982742.63	1.13	991037.36	1.12	989105.5	1.14
遗传信息处理 Genetic information processing	转译Translation	2612652.11	3.01	2617872.7	2.97	2614704	3.01
	复制和修复Replication and repair	2079265.69	2.40	2099073.8	2.38	2079876	2.39
	折叠、排序和退化Folding,sorting and degradation	1218889.45	1.41	1231163.4	1.40	1218668	1.40
环境信息处理Environmental information processing	膜运输Membrane transport	2349219.64	2.71	2396698.2	2.72	2336902	2.69
环境信息处理Environmental information processing	信号转导Signal transduction	1968830.46	2.27	2004003.8	2.27	1961715	2.26
细胞过程Cellular processes	细胞群落-原核生物Cellular community - prokaryotes	1954704.28	2.25	1986461.1	2.25	1955751	2.25