Biotechnology Bulletin ›› 2020, Vol. 36 ›› Issue (10): 142-149.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0106
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WU Ting-ting(), GUI Zhe, QIN Ying-qiu, XIE Zhi-xiong()
Received:
2020-02-06
Online:
2020-10-26
Published:
2020-11-02
Contact:
XIE Zhi-xiong
E-mail:1243070131@qq.com;zxxie@whu.edu.cn
WU Ting-ting, GUI Zhe, QIN Ying-qiu, XIE Zhi-xiong. σ Factor SigW/ Anti-σ Factor Involving in the Toxicity of Pseudomonas donghuensis HYS to Caenorhabditis elegans[J]. Biotechnology Bulletin, 2020, 36(10): 142-149.
引物名称 | 序列(5'-3') | 用途 |
---|---|---|
M13-47 | AGGGTTTTCCCAGTCACG | 回补检测 |
M13-48 | GAGCGGATAACAATTTCACAC | 回补检测 |
rsiW -up-1 | CGGAATTCGCCGGTACGA | rsiW上游同源臂扩增 |
rsiW -up-2 | CGGGATCCGATCAAGCTCATTTC | rsiW上游同源臂扩增 |
rsiW -down-1 | CGGGATCCATCACTTTGTAGCTG | rsiW下游同源臂扩增 |
rsiW -down-2 | CCCAAGCTTGAGATTGATTCT | rsiW下游同源臂扩增 |
rsiW -M-1 | GCCCTTGGCGTGATTCTTA | rsiW敲除检测 |
rsiW -M-2 | CACCTGGATGGATTAGACTCAA | rsiW敲除检测 |
rsiW -com-1 | CCAAGCTTTGTCACGCTTGTCG | HYS的rsiW片段扩增 |
rsiW -com -2 | CGGAATTCGCGGATACCTCG | HYS的rsiW片段扩增 |
sigW -up-1 | CGGGATCCACTTGCTGGTAGAA | sigW上游同源臂扩增 |
sigW -up-2 | CGGAATTCTAGTTCGTTCATTGC | sigW上游同源臂扩增 |
sigW -down-1 | CGGAATTCAAATGAGCTTGA | sigW下游同源臂扩增 |
sigW -down-2 | CCAAGCTTGTAGGCCGAGA | sigW下游同源臂扩增 |
sigW -M-1 | GCTGGCGTAGGTCGTGTTG | sigW敲除检测 |
sigW - M-2 | CTTACGCCCCTAATGGCA | sigW敲除检测 |
sigW -com-1 | CCAAGCTTGTCTGCTTCGTACAC | HYS的sigW片段扩增 |
sigW -com -2 | CGGAATTCTCGGACGGGAT | HYS的sigW片段扩增 |
sigWrsiW -up-1 | CGGGATCCCTGAACACCCTCG | sigWrsiW上游同源臂扩增 |
sigWrsiW -up-2 | CGGAATTCGTCTAGTTCGTTCATTGC | sigWrsiW上游同源臂扩增 |
sigWrsiW -down-1 | CGGAATTCGGTATCACTTTGTAGCTG | sigWrsiW下游同源臂扩增 |
sigWrsiW -down-2 | CCAAGCTTGCCTTCGAGCCGA | sigWrsiW下游同源臂扩增 |
sigWrsiW -M-1 | AGGTCGTGTTGCAGGTAGTCGTTG | sigWrsiW敲除检测 |
sigWrsiW - M-2 | GCGATGCTCCCGATAGAGGC | sigWrsiW敲除检测 |
sigWrsiW -com-1 | CCAAGCTTCACTGATCGCCTACA | HYS的sigWrsiW片段扩增 |
sigWrsiW -com -2 | CGGAATTCCGTGCGCTTCA | HYS的sigWrsiW片段扩增 |
rpoB -RT-1 | CGGGAGCGACCAAAGATCAG | HYS的RT-PCR内参基因扩增 |
rpoB - RT -2 | CGTACTCCAGGGCAGCATTG | HYS的RT-PCR内参基因扩增 |
sigW- RT -1 | GCAACACCGAAGATATCGTC | HYS的RT-PCR sigW扩增 |
sigW - RT -2 | TGCCAATCGGCACATCCA | HYS的RT-PCR sigW扩增 |
rsiW - RT -1 | TACGGTTTTACCCCGCTAGGT | HYS的RT-PCR rsiW扩增 |
rsiW -RT -2 | GTCGGTTCGCTCACCACT | HYS的RT-PCR rsiW扩增 |
rsiW -in-1 | AACGTGCGCCGTGAGTTG | HYS的rsiW转录检测 |
rsiW -in -2 | CGTCGGTTCGCTCACCACT | HYS的rsiW转录检测 |
sigW -in-1 | TGCGTCGCTTCGCCCTGT | HYS的sigW转录检测 |
sigW -in -2 | GCGACAAGCGTGACATTACCG | HYS的sigW转录检测 |
sigWrsiW- in-1 | TCGATCCTGTACCGGCAGTTT | HYS共转录检测 |
sigWrsiW-in -2 | TAGCGGGGTAAAACCGTAGG | HYS共转录检测 |
rsiW -com-3 | ATGAGCTTGATCCCGTCCGA | rsiW酵母双杂交扩增 |
rsiW -com -4 | CTACAAAGTGATACCAAGCGCCTG | rsiW酵母双杂交扩增 |
rsiW -N-com-1 | ATGAGCTTGATCCCGTCCGA | rsiW - N酵母双杂交扩增 |
rsiW -N-com -2 | TCAGCGCCGTTGCCG | rsiW - N酵母双杂交扩增 |
rsiW -C-com-1 | ATGCGCGAGGCGAGCCCT | rsiW - C酵母双杂交扩增 |
rsiW -C-com -2 | CTACAAAGTGATACCAAGCGCCTG | rsiW - C酵母双杂交扩增 |
sigW -com-3 | ATGAACGAACTAGACGACGAGC | sigW酵母双杂交扩增 |
sigW -com-4 | TCATTTCAACCTCCGCAGCG | sigW酵母双杂交扩增 |
引物名称 | 序列(5'-3') | 用途 |
---|---|---|
M13-47 | AGGGTTTTCCCAGTCACG | 回补检测 |
M13-48 | GAGCGGATAACAATTTCACAC | 回补检测 |
rsiW -up-1 | CGGAATTCGCCGGTACGA | rsiW上游同源臂扩增 |
rsiW -up-2 | CGGGATCCGATCAAGCTCATTTC | rsiW上游同源臂扩增 |
rsiW -down-1 | CGGGATCCATCACTTTGTAGCTG | rsiW下游同源臂扩增 |
rsiW -down-2 | CCCAAGCTTGAGATTGATTCT | rsiW下游同源臂扩增 |
rsiW -M-1 | GCCCTTGGCGTGATTCTTA | rsiW敲除检测 |
rsiW -M-2 | CACCTGGATGGATTAGACTCAA | rsiW敲除检测 |
rsiW -com-1 | CCAAGCTTTGTCACGCTTGTCG | HYS的rsiW片段扩增 |
rsiW -com -2 | CGGAATTCGCGGATACCTCG | HYS的rsiW片段扩增 |
sigW -up-1 | CGGGATCCACTTGCTGGTAGAA | sigW上游同源臂扩增 |
sigW -up-2 | CGGAATTCTAGTTCGTTCATTGC | sigW上游同源臂扩增 |
sigW -down-1 | CGGAATTCAAATGAGCTTGA | sigW下游同源臂扩增 |
sigW -down-2 | CCAAGCTTGTAGGCCGAGA | sigW下游同源臂扩增 |
sigW -M-1 | GCTGGCGTAGGTCGTGTTG | sigW敲除检测 |
sigW - M-2 | CTTACGCCCCTAATGGCA | sigW敲除检测 |
sigW -com-1 | CCAAGCTTGTCTGCTTCGTACAC | HYS的sigW片段扩增 |
sigW -com -2 | CGGAATTCTCGGACGGGAT | HYS的sigW片段扩增 |
sigWrsiW -up-1 | CGGGATCCCTGAACACCCTCG | sigWrsiW上游同源臂扩增 |
sigWrsiW -up-2 | CGGAATTCGTCTAGTTCGTTCATTGC | sigWrsiW上游同源臂扩增 |
sigWrsiW -down-1 | CGGAATTCGGTATCACTTTGTAGCTG | sigWrsiW下游同源臂扩增 |
sigWrsiW -down-2 | CCAAGCTTGCCTTCGAGCCGA | sigWrsiW下游同源臂扩增 |
sigWrsiW -M-1 | AGGTCGTGTTGCAGGTAGTCGTTG | sigWrsiW敲除检测 |
sigWrsiW - M-2 | GCGATGCTCCCGATAGAGGC | sigWrsiW敲除检测 |
sigWrsiW -com-1 | CCAAGCTTCACTGATCGCCTACA | HYS的sigWrsiW片段扩增 |
sigWrsiW -com -2 | CGGAATTCCGTGCGCTTCA | HYS的sigWrsiW片段扩增 |
rpoB -RT-1 | CGGGAGCGACCAAAGATCAG | HYS的RT-PCR内参基因扩增 |
rpoB - RT -2 | CGTACTCCAGGGCAGCATTG | HYS的RT-PCR内参基因扩增 |
sigW- RT -1 | GCAACACCGAAGATATCGTC | HYS的RT-PCR sigW扩增 |
sigW - RT -2 | TGCCAATCGGCACATCCA | HYS的RT-PCR sigW扩增 |
rsiW - RT -1 | TACGGTTTTACCCCGCTAGGT | HYS的RT-PCR rsiW扩增 |
rsiW -RT -2 | GTCGGTTCGCTCACCACT | HYS的RT-PCR rsiW扩增 |
rsiW -in-1 | AACGTGCGCCGTGAGTTG | HYS的rsiW转录检测 |
rsiW -in -2 | CGTCGGTTCGCTCACCACT | HYS的rsiW转录检测 |
sigW -in-1 | TGCGTCGCTTCGCCCTGT | HYS的sigW转录检测 |
sigW -in -2 | GCGACAAGCGTGACATTACCG | HYS的sigW转录检测 |
sigWrsiW- in-1 | TCGATCCTGTACCGGCAGTTT | HYS共转录检测 |
sigWrsiW-in -2 | TAGCGGGGTAAAACCGTAGG | HYS共转录检测 |
rsiW -com-3 | ATGAGCTTGATCCCGTCCGA | rsiW酵母双杂交扩增 |
rsiW -com -4 | CTACAAAGTGATACCAAGCGCCTG | rsiW酵母双杂交扩增 |
rsiW -N-com-1 | ATGAGCTTGATCCCGTCCGA | rsiW - N酵母双杂交扩增 |
rsiW -N-com -2 | TCAGCGCCGTTGCCG | rsiW - N酵母双杂交扩增 |
rsiW -C-com-1 | ATGCGCGAGGCGAGCCCT | rsiW - C酵母双杂交扩增 |
rsiW -C-com -2 | CTACAAAGTGATACCAAGCGCCTG | rsiW - C酵母双杂交扩增 |
sigW -com-3 | ATGAACGAACTAGACGACGAGC | sigW酵母双杂交扩增 |
sigW -com-4 | TCATTTCAACCTCCGCAGCG | sigW酵母双杂交扩增 |
HYS突变株编号 | HYS基因编号 | HYS插入位置 | HYS蛋白编号 |
---|---|---|---|
0201 7A | UW3_RS0125385 | 24892-24893 | WP_010226811.1 |
HYS突变株编号 | HYS基因编号 | HYS插入位置 | HYS蛋白编号 |
---|---|---|---|
0201 7A | UW3_RS0125385 | 24892-24893 | WP_010226811.1 |
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