Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (2): 122-128.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0714
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YIN Jun-lei(), ZHANG Yan-fang, ZOU Fan-yu, PAN Peng-tao(), DUAN Yan-hong, QIU Shu-xing
Received:
2020-06-10
Online:
2021-02-26
Published:
2021-02-26
Contact:
PAN Peng-tao
E-mail:lyin22@163.com;panpengtao126@163.com
YIN Jun-lei, ZHANG Yan-fang, ZOU Fan-yu, PAN Peng-tao, DUAN Yan-hong, QIU Shu-xing. Construction and Immunoprotection of sptP Deletion Mutant of Salmonella Pullorum[J]. Biotechnology Bulletin, 2021, 37(2): 122-128.
引物名称 | 引物序列(5'-3') | 扩增片段长度/bp |
---|---|---|
F1 | TGAATCAGCAGGAAGCTCTCAAAAACATACTGCAGGAATgtgtaggctggagctgcttc | 1 111 |
F2 | CTTACTTTCAGATAGTTCTAAAAGTAAGCTATGTTTTTAatgggaattagccatggtcc | |
CX1 | CCGATCTGACAAATACTTAC | 2 160或1 561或631 |
CX2 | ATCCGAACTACTTTACGC | |
H1 | TTTGGCGAGGTGCATACC | 391 |
H2 | GTGAGGCGTCTTCCAGCA |
引物名称 | 引物序列(5'-3') | 扩增片段长度/bp |
---|---|---|
F1 | TGAATCAGCAGGAAGCTCTCAAAAACATACTGCAGGAATgtgtaggctggagctgcttc | 1 111 |
F2 | CTTACTTTCAGATAGTTCTAAAAGTAAGCTATGTTTTTAatgggaattagccatggtcc | |
CX1 | CCGATCTGACAAATACTTAC | 2 160或1 561或631 |
CX2 | ATCCGAACTACTTTACGC | |
H1 | TTTGGCGAGGTGCATACC | 391 |
H2 | GTGAGGCGTCTTCCAGCA |
菌株 | 攻毒剂量/CFU | 死亡数/总数 | LD50/CFU |
---|---|---|---|
C79-13 | 1×108 | 10/10 | 3.16 × 106 |
1×107 | 9/10 | ||
1×106 | 1/10 | ||
1×105 | 0/10 | ||
C79-13ΔsptP | 1×109 | 2/10 | > 1.00 × 109 |
1×108 | 0/10 | ||
C79-13ΔsptP (pBR322-sptP) | 1×109 | 10/10 | 6.90 × 106 |
1×108 | 9/10 | ||
1×107 | 6/10 | ||
1×106 | 1/10 | ||
1×105 | 0/10 | ||
PBS | -- | 0/10 | -- |
菌株 | 攻毒剂量/CFU | 死亡数/总数 | LD50/CFU |
---|---|---|---|
C79-13 | 1×108 | 10/10 | 3.16 × 106 |
1×107 | 9/10 | ||
1×106 | 1/10 | ||
1×105 | 0/10 | ||
C79-13ΔsptP | 1×109 | 2/10 | > 1.00 × 109 |
1×108 | 0/10 | ||
C79-13ΔsptP (pBR322-sptP) | 1×109 | 10/10 | 6.90 × 106 |
1×108 | 9/10 | ||
1×107 | 6/10 | ||
1×106 | 1/10 | ||
1×105 | 0/10 | ||
PBS | -- | 0/10 | -- |
组别 | 免疫后不同天数雏鸡的平均体重/g | ||
---|---|---|---|
5 | 12 | 19 | |
免疫组 | 66.67 ± 0.82 | 125.68 ± 1.02 | 193.71 ± 1.49 |
对照组 | 65.81 ± 0.94 | 128.03 ± 1.57 | 192.86 ± 1.83 |
组别 | 免疫后不同天数雏鸡的平均体重/g | ||
---|---|---|---|
5 | 12 | 19 | |
免疫组 | 66.67 ± 0.82 | 125.68 ± 1.02 | 193.71 ± 1.49 |
对照组 | 65.81 ± 0.94 | 128.03 ± 1.57 | 192.86 ± 1.83 |
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