RNA Sequencing Analysis of Cecum Tissues of Jinghai Yellow Chickens Infected by E. tenella

doi:10.13560/j.cnki.biotech.bull.1985.2019-0257

Abstract

Abstract: This work aims to investigate the differentially expressed genes（DEGs）of cecal tissue of Jinghai yellow chickens infected with E. tenella and the molecular response process and immune response mechanism of the infection. The RNA-seq technique was used to sequence the cecal tissue of E. tenella-infected group and non-infected group on the 7th day of post-infection,and the DEGs were screened for functional and pathway enrichment analysis. The results indicated that there were 2 830 DEGs（P<0.05）between the infected and uninfected groups,of which 1 419 were up-regulated and 1 411 were down-regulated. Ten DEGs were randomly selected for qRT-PCR verification,and the results showed that the fold change of DEGs was highly consistent with the RNA-seq results（r=0.988,P<0.000）,and the coefficient of determination reached 0.975. GO analysis showed that 2 356 DEGs were functionally annotated,and the top 30 significantly enriched GO terms involved mainly cell communication,signal transduction,angiogenesis,and oxidoreductase activity. The KEGG analysis revealed that top significantly enriched signaling pathways included focal adhesions,extracellular matrix-receptor interactions and peroxisome proliferator-activated receptors. Key DEGs in these pathways include ANGPTL4,ACSL5,VEGFC,CD44,and MAKP10,etc.,suggesting that these genes would play an important role in the infection of E. tenella.

Key words: Jinghai yellow chicken, E. tenella, cecum, RNA-seq, DEGs

YU Hai-liang, ZOU Wen-bin, WANG Xiao-hui, LIN Yu-xin, DAI Guo-jun, ZHANG Tao, ZHANG Gen-xi, XIE Kai-zhou, WANG Jin-yu, SHI Hui-qiang. RNA Sequencing Analysis of Cecum Tissues of Jinghai Yellow Chickens Infected by E. tenella[J]. Biotechnology Bulletin, 2019, 35(11): 64-71.

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