An Overview of High Throughput Biological Screening Methods and Its Application

doi:10.13560/j.cnki.biotech.bull.1985.2016.06.007

Abstract

Abstract: The emergence of genomics，proteomics，metabolomics and other molecular biology studies makes the rapid attainment and assessment of large amounts of biological data become extremely important. The traditional detection methods are time-consuming and laborious，and cannot satisfy the needs of contemporary biological science research for massive biological information. High Throughput Screening(HTS)methods are significant means of quick attainment of massive biological information. This paper will make an overview of microarray chip，microfluidic chip，pyrosequencing，fluorescence polarization immunoassay，quantum dot fluorescence immunoassay and multiple PCR，outline research focus and research results of HTS methods in recent years，and briefly introduce its application in food safety，medicine and other fields.

Key words: high throughput, chip, immunoassay, food safety

ZHAI Xu-zhao, WANG Guang-bin, ZHAO Liang-tao, ZENG Hai-juan, LI Jian-wu, DING Cheng-chao, SONG Chun-mei, LIU Qing. An Overview of High Throughput Biological Screening Methods and Its Application[J]. Biotechnology Bulletin, 2016, 32(6): 38-46.

References

[1] Zhao W, Ali MM, Aguirre SD, et al. Paper-based bioassays using gold nanoparticle colorimetric probes[J]. Analytical Chemistry, 2008, 80(22)：8431-8437.
[2] 肖守军, 陈凌, 许宁. 生物芯片进展[J]. 化学进展, 2009, 21(11)：2397-2410.
[3] Zubtsova ZI, Savvateeva EN, Butvilovskaya VI, et al. Immunoassay of nine serological tumor markers on a hydrogel-based microchip[J]. Russian Journal of Bioorganic Chemistry, 2013, 39(6)：619-628.
[4] Feyzkhanova GU, Filippova MA, Talibov VO, et al. Development of hydrogel biochip for in vitro allergy diagnostics[J]. Journal of Immunological Methods, 2014, 406：51-57.
[5] Jing W, Shi X, He Y, et al. Novel plastic biochips for colorimetric detection of biomolecules[J]. Analytical & Bioanalytical Chemistry, 2012, 404(6-7)：1935-1944.
[6] Cretich M, Damin F, Chiari M, et al. Protein microarray technology：how far off is routine diagnostics?[J]. Analyst, 2014, 139, 528-542.
[7] Hessner MJ, Wang X, Hulse K, et al. Three color cDNA microarrays：quantitative assessment through the use of fluorescein-labeled probes[J]. Nucleic Acids Research, 2003, 31(4)：31-42.
[8] Delehanty JB, Ligler FS. Method for printing functional protein microarrays[J]. Biotechniques, 2003, 34(2)：380-385.
[9] Desmet C, Goff GCL, Brès JC, et al. Multiplexed immunoassay for the rapid detection of anti-tumor-associated antigens antibodies[J]. Analyst, 2011, 136(1)：2918-2924.
[10] Qiu S, Song CM, Zhao SG, et al. A new spot quality control for protein macroarray based on immunological detection[J]. Talanta, 2015, 138(1)：176-182.
[11] Mavrogiannopoulou E, Petrou PS, Koukouvinos G, et al. Improved DNA microarray detection sensitivity through immobilization of preformed in solution streptavidin/biotinylated oligonucleotide conjugates[J]. Colloids & Surfaces B Biointerfaces, 2015, 128：464-472.
[12] Kim H, Takei H, Yasuda K. Quantitative evaluation of a gold-nanoparticle labeling method for detecting target DNAs on DNA microarrays[J]. Sensors & Actuators B Chemical, 2010, 144(1)：6-10.
[13] 李浩林, 刘箐, 刘芳, 等. 基于可视化抗体宏阵列技术的出血性大肠杆菌O157∶H7定量检测[J]. 食品科学, 2014, 35(20)：185-191.
[14] Zheng GX, Li YJ, Qi LL, et al. Marine phytoplankton motility sensor integrated into a microfluidic chip for high-throughput pollutant toxicity assessment[J]. Marine Pollution Bulletin, 2014, 84(1-2)：147-154.
[15] Ertl P, Sticker D, Charwat V, et al. Lab-on-a-chip technologies for stem cell analysis[J]. Trends in Biotechnology, 2014, 32(5)：245-253.
[16] Baratchi S, Khoshmanesh K, Sacristán C, et al. Immunology on chip：promises and opportunities[J]. Biotechnology Advances, 2014, 32(2)：333-346.
[17] Roy S, Yue CY, Venkatraman SS, et al. Fabrication of smart COC chips：Advantages of N-vinylpyrrolidone(NVP)monomer over other hydrophilic monomers[J]. Sensors & Actuators B Chemical, 2013, 178(3)：86-95.
[18] 庞中华, 宋满仓, 刘莹, 等. 微流控芯片技术的现状与发展[J]. 塑料, 2010(3)：11-13.
[19] 宋满仓, 刘莹, 祝铁丽, 等. 塑料微流控芯片的注塑成型[J]. 纳米技术与精密工程, 2011, 9(4)：329-334.
[20] Matteucci M, Christiansen TL, Tanzi S, et al. Fabrication and characterization of injection molded multi level nano and microfluidic systems[J]. Microelectronic Engineering, 2013, 111(4)：294-298.
[21] Gharizadeh B, Akhras M, Nourizad N, et al. Methodological improvements of pyrosequencing technology[J]. Journal of Biotechnology, 2006, 124(3)：504-511.
[22] 叶卉, 刘云龙, 邹秉杰, 等. 焦磷酸测序中PCR引物与测序引物的设计[J]. 分析化学, 2013, 41(5)：744-748.
[23] Groth M, Huse K, Reichwald K, et al. Method for preparing single-stranded DNA templates for Pyrosequencing using vector ligation and universal biotinylated primers[J]. Analytical Biochemistry, 2006, 356(2)：194-201.
[24] Ren L, Meng M, Peng W, et al. Determination of sodium benzoate in food products by fluorescence polarization immunoassay[J]. Talanta, 2014, 121：136-143.
[25] Sheng YJ, Eremin S, Tie Jun MI, et al. The development of a fluorescence polarization immunoassay for aflatoxin detection[J]. Biomedical & Environmental Sciences, 2014, 27(2)：126-129.
[26] Beloglazova NV, Eremin SA. Rapid screening of aflatoxin B1 in beer by fluorescence polarization immunoassay[J]. Talanta, 2015, 142(1)：170-175.
[27] Zhu K, Li J, Wang Z, et al. Simultaneous detection of multiple chemical residues in milk using broad-specificity antibodies in a hybrid immunosorbent assay[J]. Biosensors & Bioelectronics, 2011, 26(5)：2716-2719.
[28] Song E, Yu M, Wang Y, et al. Multi-color quantum dot-based fluorescence immunoassay array for simultaneous visual detection of multiple antibiotic residues in milk[J]. Biosensors & Bioelectronics, 2015, 72(15)：320-325.
[29] 王洪江, 柳婷, 谢跻, 等. CdS量子点制备与单增李斯特菌抗体偶联的研究[J]. 分析化学, 2010, 38：632-637.
[30] 杨淑平, 金鑫, 郑佳, 等. 高性能CdTe/CdS核壳型量子点的制备及应用于小麦面粉中呕吐毒素的荧光免疫检测研究[J]. 化学学报, 2011, 6：687-692.
[31] 赵红庆, 苑锡铜, 黄留玉. 多重PCR技术在病原检测中的应用[J]. 生物技术通讯, 2007, 18(5)：863-865.
[32] Liu F, Tan G, Li X, et al. Simultaneous detection of four causal agents of tobacco bushy top disease by a multiplex one-step RT-PCR[J]. Journal of Virological Methods, 2014, 205c：99-103.
[33] Henegariu O, Heerema NA, Dlouhy SR, et al. Multiplex PCR：critical parameters and step-by-step protocol[J]. Biotechniques, 1997, 23(3)：504-511.
[34] Zong X, Wang W, Wei H, et al. A multiplex RT-PCR assay for simultaneous detection of four viruses from sweet cherry[J]. Scientia Horticulturae, 2014, 180：118-122.
[35] 黄银花, 胡晓湘, 徐慰倬, 等. 影响多重PCR扩增效果的因素[J]. 遗传, 2003, 25(1)：65-68.
[36] Suo B, He Y, Paoli G, et al. Development of an oligonucleotide-based microarray to detect multiple foodborne pathogens[J]. Molecular & Cellular Probes, 2010, 24(2)：77-86.
[37] Karoonuthaisiri N, Charlermroj R, Uawisetwathana U, et al. Development of antibody array for simultaneous detection of foodborne pathogens[J]. Biosensors and Bioelectronics, 2009, 24(6)：1641-1648.
[38] Sun Z, Peng Y, Zhang M, et al. Simultaneous and highly sensitive detection of six different foodborne pathogens by high-throughput suspension array technology[J]. Food Control, 2014, 40(2)：300-309.
[39] Germini A, Masola A, Carnevali P, et al. Simultaneous detection of Escherichia coli O175：H7, Salmonella spp., and Listeria monocytogenes by multiplex PCR[J]. Food Control, 2009, 20(8)：733-738.
[40] 赵新, 王永, 兰青阔, 等. 焦磷酸测序技术在4种食源性致病菌快速检测中的应用[J]. 食品与生物技术学报, 2013, 32(2)：182-188.
[41] Wang B, Wang Q, Cai Z, et al. Simultaneous, rapid and sensitive detection of three food-borne pathogenic bacteria using multicolor quantum dot probes based on multiplex fluoroimmunoassay in food samples[J]. LWT-Food Science and Technology, 2015, 61：368-376.
[42] Zhou PP, Zhang JZ, You YH, et al. Detection of genetically modified crops by combination of multiplex PCR and low-density DNA microarray1[J]. Biomedical and Environmental Sciences, 2008, 21(1)：53-62.
[43] 赵胤泽, 汪琳, 邢佑尚, 等. 3种转基因成分检测蛋白芯片的研制[J]. 植物检疫, 2011, 25(3)：1-6.
[44] Obeid PJ, Christopoulos TK, Ioannou PC. Rapid analysis of genetically modified organisms by in-house developed capillary electrophoresis chip and laser-induced fluorescence system[J]. Electrophoresis, 2004, 25(6)：922-930.
[45] 刘楠, 苏璞, 朱茂祥, 等. 高通量悬浮芯片技术检测多农兽药残留[J]. 分析化学, 2010, 38(5)：673-677.
[46] 陈爱亮, 王国青, 王艳, 等. 兽药残留蛋白芯片检测系统的研制和应用[J]. 中国医疗器械信息, 2008, 14(8)：33-36.
[47] 郭红斌, 陈国平, 兰文升, 等. 一种用于有机磷农药检测的微流控传感器[J]. 传感器与微系统, 2011, 30(6)：84-86.
[48] Xu ZL, Qiang W, Lei HT, et al. A simple, rapid and high-throughput fluorescence polarization immunoassay for simultaneous detection of organophosphorus pesticides in vegetable and environmental water samples[J]. Analytica Chimica Acta, 2011, 708(1-2)：123-129.
[49] Huang W, Hu C, Zeng H, et al. Novel systemic lupus erythemato-sus autoantigens identified by human protein microarray technol-ogy[J]. Biochemical & Biophysical Research Communications, 2012, 418(2)：241-246.
[50] Yang Z, Chevolot Y, Ataman-?nal Y, et al. Cancer biomarkers detection using 3D microstructured protein chip：implementation of customized multiplex immunoassay[J]. Sensors and Actuators B Chemical, 2012, 175(12)：2228.
[51] Kang JH, Vanderstichele H, Trojanowski JQ, et al. Simultaneous analysis of cerebrospinal fluid biomarkers using microsphere-based xMAP multiplex technology for early detection of Alzheimer’s disease[J]. Methods, 2012, 56(4)：484-493.
[52] Liang Z, Dong M, Cheng Q, et al. Gestational diabetes mellitus screening based on the gene chip technique[J]. Diabetes Rese-arch & Clinical Practice, 2010, 89(2)：167-173.
[53] Tian J, Zhou L, Zhao Y, et al. Multiplexed detection of tumor markers with multicolor quantum dots based on fluorescence polarization immunoassay[J]. Talanta, 2012, 92(1)：72-77.
[54] Smith AM, Dave SS, True L, et al. Multicolor quantum dots for molecular diagnostics of cancer[J]. Expert Review of Molecular Diagnostics, 2014, 6(2)：231-244.
[55] Hallur V, Sharma M, Sethi S, et al. Development and evaluation of multiplex PCR in rapid diagnosis of abdominal tuberculosis[J]. Diagnostic Microbiology & Infectious Disease, 2013, 76(1)：51-55.
[56] Li CC, Lo HY, Hsiang CY, et al. DNA microarray analysis as a tool to investigate the therapeutic mechanisms and drug development of Chinese medicinal herbs[J]. Biomedicine, 2012, 2(1)：10-16.
[57] 郑允焕, 吴建璋, 邵建波, 等. 用于药物筛选的微流控细胞阵列芯片[J]. 生物工程学报, 2009, 25(5)：779-785.
[58] Li X, Korir NK, Liu L, et al. Microarray analysis of differentially expressed genes engaged in fruit development between Prunus mume and Prunus armeniaca[J]. Journal of Plant Physiology, 2012, 169(17)：1776-1788.
[59] 谭小勇, 阮小蕾, 吴丽婷, 等. 香蕉3种病毒的多重PCR检测体系[J]. 园艺学报, 2010, 37(5)：829-834.
[60] 井赵斌, 程积民, 张宝泉, 等. 基于454焦磷酸测序法的典型草原土壤真核生物多样性[J]. 草业科学, 2013, 30(11)：1690-1697.
[61] Divne AM, Edlund H, Allen M. Forensic analysis of autosomal STR markers using Pyrosequencing[J]. Forensic Science International Genetics, 2010, 4(2)：122-129.
[62] 曾立波, 陈连康, 胡小龙, 等. 定量检测毒品的蛋白芯片的研发[J]. 中国司法鉴定, 2012(1)：16-19.