Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (5): 28-37.doi: 10.13560/j.cnki.biotech.bull.1985.2020-1203

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Impacts of Cymbidium goeringii’s miR396 Overexpression on the Leaf Growth,Photosynthesis and Chlorophyll Fluorescence in Arabidopsis thaliana

XU Zi-han1(), LIU Qian1, MIAO Da-peng1, CHEN Yue2, HU Feng-rong1()   

  1. 1. College of Landscape and Architecture,Nanjing Forestry University,Nanjing 210037
    2. Institute of Horticulture,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021
  • Received:2020-09-23 Online:2021-05-26 Published:2021-06-11
  • Contact: HU Feng-rong E-mail:406922521@qq.com;hufengrong2003@sina.com

Abstract:

The objective of this work is to study the role of Cymbidium goeringii’s miR396(cgo-miR396)in the growth and development of plant leaves,and to provide a new research idea for the cultivation and production of C. goeringii. The precursor of gene cgo-MIR396 was overexpressed in Arabidopsis thaliana,and the changes of its morphological indexes were observed and the corresponding photosynthetic and chlorophyll fluorescence parameters were determined. As results,gene cgo-miR396 was in the highest expression level in leaves at flowering stage,and the overexpression of cgo-miR396 significantly increased the leaf length,leaf width,leaf area and plant height of Arabidopsis thaliana,while significantly reduced the chlorophyll content,stomatal conductance(gs)and transpiration rate(Tr)of leaves,and improved the water use efficiency(WUE). In addition,the overexpression of this gene also resulted in the decrease in the quantum yield(φEo),performance index(PIABS),thrust force(DFABS)and other chlorophyll fluorescence parameters of the light absorbed by the reaction center for electron transfer. In conclusion,cgo-miR396 mainly plays a role in the reproductive growth period and appropriately reduces stomatal conductance of plant leaves and some properties of the PSII receptor side while the net photosynthetic efficiency of plants and promoting leaf growth is ensured.

Key words: Cymbidium goeringii, miR396, Arabidopsis thaliana, photosynthesis, chlorophyll fluorescence