Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (5): 28-37.doi: 10.13560/j.cnki.biotech.bull.1985.2020-1203
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XU Zi-han1(), LIU Qian1, MIAO Da-peng1, CHEN Yue2, HU Feng-rong1()
Received:
2020-09-23
Online:
2021-05-26
Published:
2021-06-11
Contact:
HU Feng-rong
E-mail:406922521@qq.com;hufengrong2003@sina.com
XU Zi-han, LIU Qian, MIAO Da-peng, CHEN Yue, HU Feng-rong. Impacts of Cymbidium goeringii’s miR396 Overexpression on the Leaf Growth,Photosynthesis and Chlorophyll Fluorescence in Arabidopsis thaliana[J]. Biotechnology Bulletin, 2021, 37(5): 28-37.
株系 | 叶绿素a | 叶绿素b | 叶绿素 a+b | 叶绿素a/b |
---|---|---|---|---|
Line | Chla/(mg·g-1) | Chlb/(mg·g-1) | Chl(a+b)/(mg·g-1) | Chla/b |
WT | 1.797±0.09a | 0.467±0.02a | 2.267±0.11a | 3.853±0.04a |
Line1 | 1.300±0.09b | 0.340±0.03b | 1.637±0.12b | 3.883±0.13a |
Line2 | 1.280±0.10b | 0.330±0.04b | 1.607±0.14b | 3.923±0.18a |
Line3 | 1.300±0.06b | 0.337±0.02b | 1.640±0.08b | 3.857±0.06a |
Line4 | 1.497±0.05b | 0.397±0.03ab | 1.893±0.08b | 3.770±0.12a |
Table 1 Determination of chlorophyll content
株系 | 叶绿素a | 叶绿素b | 叶绿素 a+b | 叶绿素a/b |
---|---|---|---|---|
Line | Chla/(mg·g-1) | Chlb/(mg·g-1) | Chl(a+b)/(mg·g-1) | Chla/b |
WT | 1.797±0.09a | 0.467±0.02a | 2.267±0.11a | 3.853±0.04a |
Line1 | 1.300±0.09b | 0.340±0.03b | 1.637±0.12b | 3.883±0.13a |
Line2 | 1.280±0.10b | 0.330±0.04b | 1.607±0.14b | 3.923±0.18a |
Line3 | 1.300±0.06b | 0.337±0.02b | 1.640±0.08b | 3.857±0.06a |
Line4 | 1.497±0.05b | 0.397±0.03ab | 1.893±0.08b | 3.770±0.12a |
株系 | 初始荧光 | 最大荧光 | 可变荧光 | PS II最大光化学效率 | PS II 潜在光化学效率 |
---|---|---|---|---|---|
Line | Fo | Fm | Fv | Fv/Fm | Fv/Fo |
WT | 547.5±9.93a | 3229.5±35.62a | 2682.0±27.04a | 0.831±0.002a | 4.905±0.05a |
Line1 | 541.7±26.21a | 3252.3±115.61a | 2710.7±104.80a | 0.833±0.007a | 5.023±0.27a |
Line2 | 537.5±0.29a | 3115.5±79.39a | 2578.0±79.10a | 0.828±0.004a | 4.796±0.14a |
Line3 | 564.7±21.36a | 3104.3±17.64a | 2539.7±5.46a | 0.818±0.006a | 4.511±0.18a |
Line4 | 551.0±8.89a | 3282.0±96.16a | 2731.0±87.47a | 0.832±0.002a | 4.954±0.08a |
Table 2 Rapid determination of chlorophyll fluorescence parameters
株系 | 初始荧光 | 最大荧光 | 可变荧光 | PS II最大光化学效率 | PS II 潜在光化学效率 |
---|---|---|---|---|---|
Line | Fo | Fm | Fv | Fv/Fm | Fv/Fo |
WT | 547.5±9.93a | 3229.5±35.62a | 2682.0±27.04a | 0.831±0.002a | 4.905±0.05a |
Line1 | 541.7±26.21a | 3252.3±115.61a | 2710.7±104.80a | 0.833±0.007a | 5.023±0.27a |
Line2 | 537.5±0.29a | 3115.5±79.39a | 2578.0±79.10a | 0.828±0.004a | 4.796±0.14a |
Line3 | 564.7±21.36a | 3104.3±17.64a | 2539.7±5.46a | 0.818±0.006a | 4.511±0.18a |
Line4 | 551.0±8.89a | 3282.0±96.16a | 2731.0±87.47a | 0.832±0.002a | 4.954±0.08a |
叶面积 | 叶长 | 叶宽 | 株高 | 叶绿素a+b | 叶绿素a/b | Ci | gs | A | E | WUE | Fo | Fm | Fv | Fv/Fm | Fv/Fo | ABS/RC | DIo/RC | TRo/RC | ETo/RC | φPo | ψo | φEo | PI ABS | DF ABS | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
叶面积 | 1 | ||||||||||||||||||||||||
叶长 | 0.883** | 1 | |||||||||||||||||||||||
叶宽 | 0.953** | 0.733** | 1 | ||||||||||||||||||||||
株高 | 0.464 | 0.470 | 0.408 | 1 | |||||||||||||||||||||
叶绿素a+b | -0.194 | -0.046 | -0.284 | -0.281 | 1 | ||||||||||||||||||||
叶绿素a/b | 0.017 | -0.239 | 0.179 | -0.037 | -0.257 | 1 | |||||||||||||||||||
Ci | -0.206 | -0.361 | -0.154 | -0.299 | 0.290 | 0.471 | 1 | ||||||||||||||||||
gs | -0.551* | -0.579* | -0.524* | -0.269 | 0.426 | 0.363 | 0.778** | 1 | |||||||||||||||||
A | -0.004 | -0.033 | 0.070 | 0.139 | -0.049 | -0.153 | -0.072 | -0.018 | 1 | ||||||||||||||||
E | -0.412 | -0.488 | -0.364 | -0.332 | 0.326 | 0.312 | 0.868** | 0.890** | 0.087 | 1 | |||||||||||||||
WUE | 0.261 | 0.384 | 0.227 | 0.419 | -0.279 | -0.420 | -0.922** | -0.797** | 0.177 | -0.921** | 1 | ||||||||||||||
Fo | 0.143 | 0.268 | 0.118 | 0.034 | 0.004 | 0.178 | 0.218 | 0.248 | 0.360 | 0.214 | -0.169 | 1 | |||||||||||||
Fm | -0.137 | -0.029 | -0.254 | -0.199 | 0.291 | -0.032 | 0.261 | 0.233 | 0.286 | 0.226 | -0.173 | 0.327 | 1 | ||||||||||||
Fv | -0.171 | -0.083 | -0.289 | -0.215 | 0.304 | -0.068 | 0.230 | 0.196 | 0.229 | 0.195 | -0.148 | 0.146 | 0.983** | 1 | |||||||||||
Fv/Fm | -0.249 | -0.276 | -0.323 | -0.191 | 0.227 | -0.177 | 0.030 | -0.015 | -0.085 | 0.007 | -0.004 | -0.629* | 0.529* | 0.677** | 1 | ||||||||||
Fv/Fo | -0.256 | -0.289 | -0.319 | -0.195 | 0.237 | -0.183 | 0.029 | -0.027 | -0.062 | -0.003 | 0.011 | -0.636* | 0.518* | 0.667** | 0.996** | 1 | |||||||||
ABS/RC | 0.346 | 0.408 | 0.352 | 0.271 | -0.243 | 0.052 | -0.308 | -0.314 | 0.007 | -0.453 | 0.407 | 0.419 | -0.177 | -0.267 | -0.516* | -0.510 | 1 | ||||||||
DIo/RC | 0.350 | 0.369 | 0.417 | 0.239 | -0.267 | 0.240 | -0.110 | -0.115 | 0.057 | -0.193 | 0.153 | 0.631* | -0.409 | -0.552* | -0.901** | -0.893** | 0.824** | 1 | |||||||
TRo/RC | 0.302 | 0.377 | 0.278 | 0.253 | -0.202 | -0.044 | -0.366 | -0.371 | -0.018 | -0.522* | 0.478 | 0.266 | -0.044 | -0.098 | -0.269 | -0.264 | 0.962** | 0.639* | 1 | ||||||
ETo/RC | -0.049 | 0.092 | -0.164 | -0.348 | 0.216 | -0.159 | -0.140 | -0.033 | -0.227 | -0.287 | 0.191 | 0.218 | 0.369 | 0.343 | 0.111 | 0.090 | 0.461 | 0.138 | 0.560* | 1 | |||||
φPo | -0.273 | -0.261 | -0.368 | -0.167 | 0.222 | -0.325 | -0.055 | -0.058 | -0.075 | -0.040 | 0.066 | -0.648** | 0.490 | 0.640* | 0.986** | 0.981** | -0.506 | -0.905** | -0.252 | 0.129 | 1 | ||||
ψo | -0.277 | -0.159 | -0.398 | -0.607* | 0.405 | -0.159 | 0.098 | 0.228 | -0.259 | 0.032 | -0.116 | 0.080 | 0.476 | 0.482 | 0.319 | 0.291 | -0.123 | -0.281 | -0.033 | 0.810** | 0.330 | 1 | |||
φEo | -0.309 | -0.196 | -0.439 | -0.592* | 0.415 | -0.211 | 0.078 | 0.197 | -0.250 | 0.021 | -0.092 | -0.050 | 0.533* | 0.568* | 0.484 | 0.457 | -0.212 | -0.434 | -0.079 | 0.769** | 0.496 | 0.983** | 1 | ||
PI ABS | -0.343 | -0.357 | -0.402 | -0.624* | 0.430 | 0.075 | 0.310 | 0.301 | -0.214 | 0.233 | -0.314 | -0.240 | 0.605* | 0.680** | 0.710** | 0.697** | -0.559* | -0.707** | -0.420 | 0.395 | 0.657** | 0.773** | 0.838** | 1 | |
DF ABS | -0.313 | -0.334 | -0.379 | -0.580* | 0.430 | 0.070 | 0.302 | 0.285 | -0.237 | 0.211 | -0.297 | -0.267 | 0.596* | 0.676** | 0.727** | 0.712** | -0.561* | -0.721** | -0.416 | 0.394 | 0.676** | 0.768** | 0.837** | 0.997** | 1 |
Table 3 Correlation coefficient matrix of each single index
叶面积 | 叶长 | 叶宽 | 株高 | 叶绿素a+b | 叶绿素a/b | Ci | gs | A | E | WUE | Fo | Fm | Fv | Fv/Fm | Fv/Fo | ABS/RC | DIo/RC | TRo/RC | ETo/RC | φPo | ψo | φEo | PI ABS | DF ABS | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
叶面积 | 1 | ||||||||||||||||||||||||
叶长 | 0.883** | 1 | |||||||||||||||||||||||
叶宽 | 0.953** | 0.733** | 1 | ||||||||||||||||||||||
株高 | 0.464 | 0.470 | 0.408 | 1 | |||||||||||||||||||||
叶绿素a+b | -0.194 | -0.046 | -0.284 | -0.281 | 1 | ||||||||||||||||||||
叶绿素a/b | 0.017 | -0.239 | 0.179 | -0.037 | -0.257 | 1 | |||||||||||||||||||
Ci | -0.206 | -0.361 | -0.154 | -0.299 | 0.290 | 0.471 | 1 | ||||||||||||||||||
gs | -0.551* | -0.579* | -0.524* | -0.269 | 0.426 | 0.363 | 0.778** | 1 | |||||||||||||||||
A | -0.004 | -0.033 | 0.070 | 0.139 | -0.049 | -0.153 | -0.072 | -0.018 | 1 | ||||||||||||||||
E | -0.412 | -0.488 | -0.364 | -0.332 | 0.326 | 0.312 | 0.868** | 0.890** | 0.087 | 1 | |||||||||||||||
WUE | 0.261 | 0.384 | 0.227 | 0.419 | -0.279 | -0.420 | -0.922** | -0.797** | 0.177 | -0.921** | 1 | ||||||||||||||
Fo | 0.143 | 0.268 | 0.118 | 0.034 | 0.004 | 0.178 | 0.218 | 0.248 | 0.360 | 0.214 | -0.169 | 1 | |||||||||||||
Fm | -0.137 | -0.029 | -0.254 | -0.199 | 0.291 | -0.032 | 0.261 | 0.233 | 0.286 | 0.226 | -0.173 | 0.327 | 1 | ||||||||||||
Fv | -0.171 | -0.083 | -0.289 | -0.215 | 0.304 | -0.068 | 0.230 | 0.196 | 0.229 | 0.195 | -0.148 | 0.146 | 0.983** | 1 | |||||||||||
Fv/Fm | -0.249 | -0.276 | -0.323 | -0.191 | 0.227 | -0.177 | 0.030 | -0.015 | -0.085 | 0.007 | -0.004 | -0.629* | 0.529* | 0.677** | 1 | ||||||||||
Fv/Fo | -0.256 | -0.289 | -0.319 | -0.195 | 0.237 | -0.183 | 0.029 | -0.027 | -0.062 | -0.003 | 0.011 | -0.636* | 0.518* | 0.667** | 0.996** | 1 | |||||||||
ABS/RC | 0.346 | 0.408 | 0.352 | 0.271 | -0.243 | 0.052 | -0.308 | -0.314 | 0.007 | -0.453 | 0.407 | 0.419 | -0.177 | -0.267 | -0.516* | -0.510 | 1 | ||||||||
DIo/RC | 0.350 | 0.369 | 0.417 | 0.239 | -0.267 | 0.240 | -0.110 | -0.115 | 0.057 | -0.193 | 0.153 | 0.631* | -0.409 | -0.552* | -0.901** | -0.893** | 0.824** | 1 | |||||||
TRo/RC | 0.302 | 0.377 | 0.278 | 0.253 | -0.202 | -0.044 | -0.366 | -0.371 | -0.018 | -0.522* | 0.478 | 0.266 | -0.044 | -0.098 | -0.269 | -0.264 | 0.962** | 0.639* | 1 | ||||||
ETo/RC | -0.049 | 0.092 | -0.164 | -0.348 | 0.216 | -0.159 | -0.140 | -0.033 | -0.227 | -0.287 | 0.191 | 0.218 | 0.369 | 0.343 | 0.111 | 0.090 | 0.461 | 0.138 | 0.560* | 1 | |||||
φPo | -0.273 | -0.261 | -0.368 | -0.167 | 0.222 | -0.325 | -0.055 | -0.058 | -0.075 | -0.040 | 0.066 | -0.648** | 0.490 | 0.640* | 0.986** | 0.981** | -0.506 | -0.905** | -0.252 | 0.129 | 1 | ||||
ψo | -0.277 | -0.159 | -0.398 | -0.607* | 0.405 | -0.159 | 0.098 | 0.228 | -0.259 | 0.032 | -0.116 | 0.080 | 0.476 | 0.482 | 0.319 | 0.291 | -0.123 | -0.281 | -0.033 | 0.810** | 0.330 | 1 | |||
φEo | -0.309 | -0.196 | -0.439 | -0.592* | 0.415 | -0.211 | 0.078 | 0.197 | -0.250 | 0.021 | -0.092 | -0.050 | 0.533* | 0.568* | 0.484 | 0.457 | -0.212 | -0.434 | -0.079 | 0.769** | 0.496 | 0.983** | 1 | ||
PI ABS | -0.343 | -0.357 | -0.402 | -0.624* | 0.430 | 0.075 | 0.310 | 0.301 | -0.214 | 0.233 | -0.314 | -0.240 | 0.605* | 0.680** | 0.710** | 0.697** | -0.559* | -0.707** | -0.420 | 0.395 | 0.657** | 0.773** | 0.838** | 1 | |
DF ABS | -0.313 | -0.334 | -0.379 | -0.580* | 0.430 | 0.070 | 0.302 | 0.285 | -0.237 | 0.211 | -0.297 | -0.267 | 0.596* | 0.676** | 0.727** | 0.712** | -0.561* | -0.721** | -0.416 | 0.394 | 0.676** | 0.768** | 0.837** | 0.997** | 1 |
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