Cloning,Expression and Bioinformatics Analyses of CpBURP from Commelina purpurea

doi:10.13560/j.cnki.biotech.bull.1985.2020-1572

Abstract

Abstract:

BURP protein plays an important role in the growth of plant and resistance to abiotic stress. Cloning a CpBURP and analyzing its role in Commelina purpurea may lay a foundation for further deeply studying the gene BURP in C. purpurea. Screening was performed based on the C. purpurea transcriptome database and a CpBURP was cloned,and then its bioinformatics and expressions were analyzed. The result showed the CpBURP of 1 383 bp encoded a protein with 460 amino acids. The hydrophilic CpBURP protein contained a BURP conserved domain and 50 phosphorylation sites,but no transmembrane structure and signal peptide region. It is predicted that CpBURP protein acted on the cytoplasm and peroxisomes. Phylogenetic tree analysis revealed that CpBURP protein and 13 plant BURP proteins were grouped into 6 subcategories,the CpBURP protein had the closest affinity to the BURP protein in Brassica napus. The results of qRT-PCR demonstrated that the expression of CpBURP in the root was higher than that in the stem and leaf. Under the Cu²⁺ stress,the expression of CpBURP in the roots was significantly higher than that in the control group. CpBURP may play an important role in the response of C. purpurea to Cu²⁺ stress.

Key words: Commelina purpurea, CpBURP, gene cloning, bioinformatics, expression analysis

PENG Guo-ying, LU Shan, HUANG Chao, YANG Kun, WAN Wei, HUANG Chang-gan. Cloning,Expression and Bioinformatics Analyses of CpBURP from Commelina purpurea[J]. Biotechnology Bulletin, 2021, 37(9): 125-131.

Figures/Tables 10

Fig.1 Amplification procedure of qRT-PCR

Fig.2 Amplification and PCR detection of CpBURP A: PCR amplification of CpBURP; B: PCR detection of CpBURP bacterial fluid; M: DNA Marker DL2 000; 1-2: blank; 3-6: CpBURP gene

Fig. 3 CpBURP nucleotide sequence and its encoded amino acid sequence

Fig.4 Hydrophilic and hydrophobic analysis of CpBURP protein The hydrophilic/hydrophobic values are expressed by negative/positive scores respectively

Fig.5 Conserved domain of CpBURP protein

Fig. 6 Phosphorylation sites of CpBURP protein

Fig.7 Analysis of the secondary and tertiary structure of CpBURP protein A: Secondary structure. B: Tertiary structure

Fig.8 Transmembrane structure and signal peptide predic-tion of CpBURP A: Transmembrane structure; B: signal peptide prediction

Fig.9 Phylogenetic tree analysis of BURP proteins and CpBURP protein of different species

Fig.10 Expression changes of CpBURP under Cu2+ stress

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