Fig. 1 Length distribution of sRNA

Fig. 2 Expressions of sRNA in A.veronii C4 wild type

Fig. 3 Secondary structure of sRNA N155

Fig. 4 Expressions of sRNA N155 and its upstream and downstream genes

Fig. 5 RT-qPCR analysis of sRNA N155

Fig. 6 cDNA agarose gel electrophoresis M:DL5000 DNA marker. 1-2 are the bands of internal reference gene GyrB of Escherichia coli of WT+pACYC. 3-4 are the bands of internal reference gene GyrB of WT+ pACYCDuet-1∷psRNAN155-sRNA N155. 5-6 are RT products of sRNA N155 of WT+pACYC. 7-8 are RT products of sRNA N155 of WT+pACYCDuet-1∷psRNAN155-sRNA N155,about 110 bp

Fig. 7 Multiple sequence alignment of sRNA N155 gene

Fig. 8 PCR verification of sRNA N155 gene knockout vector pRE112-ΔsRNA N155 M:DL5000 DNA marker. 1 is a negative control. 2 is a positive control. 3-8 are plasmid PCR products of pRE112-ΔsRNA N155 gene knockout,the target band is about 1 200 bp

Fig. 9 22% sucrose plate screening M:DL5000 DNA marker. 1:A. veronii C4 genome positive control. 22:pRE112 plasmid positive control. 2-13:Knockout strain verification,where 2-3,10-11 are the same as expected,all are lower than the wild type. 14-21 are pRE112-specific primer verification,indicating that the pRE112 plasmid has been successfully lost

Fig. 10 PCR verification of A.veronii sRNA N155 knockout strain M:DL5000 DNA marker. 1:A.veronii C4 genome positive control. 2-5:sRNA N155 knockout strain PCR verification

Fig. 11 Determination of the growth curve of A.veronii C4 wild type and ΔsRNA N155

Fig. 12 Effect of knocking out the sRNA N155 gene on bacterial motility 1:A.veronii C4 wild-type strain. 2:Δhfq knockout strain. 3:ΔsRNA N155 knockout strain. 4:Δhfq-pBBR-hfq complement strain