Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (5): 123-135.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1074

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Non-targeted Metabolomics Analysis of Benzo(α)pyrene by Microorganisms in Kefir Grains

GULJAMAL·Aisa 1(), XING Jun2, LI An1, ZHANG Rui1()   

  1. 1. Xinjiang Special Environmental Species Protection and Regulation Biology Laboratory,School of Life Sciences,Xinjiang Normal University,Urumqi 830054
    2. College of Life Sciences and Technology,Urumqi 830000
  • Received:2021-08-23 Online:2022-05-26 Published:2022-06-10
  • Contact: ZHANG Rui E-mail:3308611513@qq.com;zhrui6@163.com

Abstract:

In order to understand the mechanism of microbial action on the polycyclic aromatic hydrocarbon benzo(α)pyrene in the traditional fermenter Kefir grains from Xinjiang,and the characteristic changes in the metabolites of Kefir grains in fermented milk under the stress of 50 mg/L benzo(α)pyrene were investigated. Using non-targeted metabonomics technology,combined with principal component analysis and partial minor binary discriminant analysis,it was confirmed that phenylacetic acid,phenol,protocatechuic acid,diisopropyl phthalate and phthalic acid were intermediate metabolic characteristic products. A total of 56 metabolic pathways were annotated in the KEGG database,among which there were 3 key pathways with high enrichment of metabolites,high significance and related to amino acid metabolism. Intermediate metabolites cleaved the benzene ring under the action of a series of enzymes such as dehydrogenase,dioxygenase,and dehydroisomerase. Therefore,it is inferred that the presence of microbial strains in the Kefir grains may have degraded benzo(α)pyrene through the“naphthalene pathway”,the“phthalic acid pathway”and the“phenylalanine pathway”. Thus study may provide a certain theoretical basis for the action mechanism of microorganisms on benzo(α)pyrene,may propose possible biological control strategies for reducing the harms of benzo(α)pyrene to the human body,and may provide research basis for the rational development and application of kefir fermented dairy products in the food industry.

Key words: Kefir grains, benzo(α)pyrene, metabolomics, metabolic pathways