Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (6): 112-119.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1338
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ZHOU Guo-yan(), YIN Shan-shan, GAO Jia-xin, WU Chun-cheng, YAN Li-ying, XIE Yang()
Received:
2021-10-23
Online:
2022-06-26
Published:
2022-07-11
Contact:
XIE Yang
E-mail:1053165570@qq.com;xieyangly123@163.com
ZHOU Guo-yan, YIN Shan-shan, GAO Jia-xin, WU Chun-cheng, YAN Li-ying, XIE Yang. Identification of AHP Gene Family in Cucumis sativus and Its Expression Analysis Under Abiotic Stress[J]. Biotechnology Bulletin, 2022, 38(6): 112-119.
引物名称Primer name | 引物序列Primer sequence(5'-3') |
---|---|
CsAHP1-F | TTGTTGAAGTGGTGTCTC |
CsAHP1-R | CCTATGCTTGAACTGCTT |
CsAHP2-F | TACTACAGAGACTCATCCA |
CsAHP2-R | GGCATCCAACTTATTGAAG |
CsAHP3-F | CCAATTCCTTCGCTACAA |
CsAHP3-R | ATCAATACACTTCTGCTCAA |
CsAHP4-F | AAGACAGTGGATTCAGTAT |
CsAHP4-R | TAGCGATTCTCAGTTTATTG |
CsAHP5-F | TTTGAGCAGGGTTATCTTG |
CsAHP5-R | GTATGAGTCTGGCTGAATC |
CsAHP6-F | GAACCGATTACTTGCCTTA |
CsAHP6-R | TCATCTTGGAGTTGTTGTAG |
CsAHP7-F | ATTGTTGAGGCAGATGAA |
CsAHP7-R | CTATATGAATCACGGTAGCA |
Cs-actin-F | ATTGTTCTCAGTGGTGGTTCTAC |
Cs-actin-R | CCTTTGAGATCCACATCTGCT |
Table 1 qRT-PCR primer design
引物名称Primer name | 引物序列Primer sequence(5'-3') |
---|---|
CsAHP1-F | TTGTTGAAGTGGTGTCTC |
CsAHP1-R | CCTATGCTTGAACTGCTT |
CsAHP2-F | TACTACAGAGACTCATCCA |
CsAHP2-R | GGCATCCAACTTATTGAAG |
CsAHP3-F | CCAATTCCTTCGCTACAA |
CsAHP3-R | ATCAATACACTTCTGCTCAA |
CsAHP4-F | AAGACAGTGGATTCAGTAT |
CsAHP4-R | TAGCGATTCTCAGTTTATTG |
CsAHP5-F | TTTGAGCAGGGTTATCTTG |
CsAHP5-R | GTATGAGTCTGGCTGAATC |
CsAHP6-F | GAACCGATTACTTGCCTTA |
CsAHP6-R | TCATCTTGGAGTTGTTGTAG |
CsAHP7-F | ATTGTTGAGGCAGATGAA |
CsAHP7-R | CTATATGAATCACGGTAGCA |
Cs-actin-F | ATTGTTCTCAGTGGTGGTTCTAC |
Cs-actin-R | CCTTTGAGATCCACATCTGCT |
Fig. 2 Phylogenetic tree analysis of AHP family members in C. sativus,Arabidopsis thaliana and Cucurbitaceae Cs:黄瓜Cucumis sativus L.;At:拟南芥Arabidopsis thaliana L.;Cmo:南瓜Cucurbita moschata Duch L.;Cla:西瓜Citrullus vulgaris Schrad L.;MEL:甜瓜Cucumis melo L.;Bhi:冬瓜Benincasa hispida L.;Cp:西葫芦Cucurbita pepo L.;Lsi:葫芦 Lagenaria siceraria L.
Fig. 5 Expression pattern analysis of AHP family in abiotic stress and different developmental stages of C. sativus a:Salt stress. b:Silicon stress. c:Temperature and light stress. d:Expression patterns in different organizations. e:Expression patterns of genes during anthesis
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