Effects of Trichoderma harzianum ST02 on the Growth of Peppermint and Physicochemical Properties of Root Zone Soil Under Salt Stress

doi:10.13560/j.cnki.biotech.bull.1985.2021-1251

Abstract

Abstract:

The salt-resistant Trichoderma harzianum strain ST02 and salt-resistant peppermint were used in this study to analyze the effects of Trichoderma on plant salt resistance and saline soil improvement. The physiological and growth traits of peppermint,the soil physicochemical property and soil enzyme activity of root zone soil under salt stress were analyzed. And the main soil factors related to the physiological and growth of peppermint were analyzed through the correlation analysis and principal component analysis（PCA）. The results showed ST02 relieved the inhibition of NaCl on peppermint growth and photosynthesis and promoted its growth and photosynthesis intensity under salt stress. When treated with NaCl,ST02 reduced the accumulation of maldehyde aldehyde（MDA）,soluble protein（SP）and proline（Pro）in peppermint cells,enhanced superoxide dismutase（SOD）,peroxide dismutase（POD）,and catalase（CAT）activities,and enhanced the salt resistance of peppermint through antioxidant response. The physiological/growth comprehensive index（PGCI）of peppermint treated with NaCl was -2.391,ST02 promoted the physiological and growth of peppermint,as index was -0.025. ST02 influenced the soil physicochemical properties for root zone soil of peppermint under NaCl stress,reduced salinity by 17.36%,and significantly enhanced the content of alkali-hydrolyzed nitrogen（AN）. The soil enzyme activity for the root zone soil of peppermint was also affected by ST02 strain,and the activity of soil cellulase（SCL）and catalase（SCAT）increased remarkably. The soil physicochemical property and soil enzyme activity affected the physiological and growth traits under NaCl stress through the correlation analysis. And the main soil factor for physiological and growth of peppermint was：EC（electrical conductance）> SCL >SSC（soil saccharase）/SNEP（soil neutrality phosphatase）>AP（available phosphorus）>pH.

Key words: salt-resistant Trichoderma, salt-resistant plant, Mentha×piperita L., physiological and growth index, soil physicochemical property, soil enzyme activity

ZHAO Zhong-juan, YANG Kai, HU Jin-dong, WEI Yan-li, LI Ling, XU Wei-sheng, LI Ji-shun. Effects of Trichoderma harzianum ST02 on the Growth of Peppermint and Physicochemical Properties of Root Zone Soil Under Salt Stress[J]. Biotechnology Bulletin, 2022, 38(7): 224-235.

Figures/Tables 12

References 27

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分组Group		处理方法Processing method
实验组 Experimental group	NaCl处理 NaCl treatment	椒样薄荷茎段扦插7 d后,用200 mL水浇灌;再7 d后,用200 mmol/L NaCl溶液200 mL浇灌,每隔3 d 进行1次NaCl处理,连续浇灌3次后用正常水浇灌
	ST02处理 ST02 treatment	椒样薄荷茎段扦插7 d后,将ST02菌剂用水按1∶100（V∶V）比例混匀（活菌数2×10⁷ CFU/g）制成菌液,用200 mL菌液进行浇灌;ST02处理7 d后,NaCl处理组用NaCl浇灌的同一时间,用正常水浇灌
	NaCl+ST02处理 NaCl+ST02 treatment	椒样薄荷茎段扦插7 d后,将ST02菌剂用水按1∶100（V∶V）比例混匀（活菌数2×10⁷ CFU/g）制成菌液,用200 mL菌液进行浇灌;ST02处理7 d后,用200 mmol/L NaCl溶液200 mL浇灌,每隔3 d进行1次NaCl处理,连续浇灌3次后用正常水浇灌
对照组Control		实验组用ST02菌液和NaCl处理的同时,用200 mL水浇灌

分组Group		处理方法Processing method
实验组 Experimental group	NaCl处理 NaCl treatment	椒样薄荷茎段扦插7 d后,用200 mL水浇灌;再7 d后,用200 mmol/L NaCl溶液200 mL浇灌,每隔3 d 进行1次NaCl处理,连续浇灌3次后用正常水浇灌
	ST02处理 ST02 treatment	椒样薄荷茎段扦插7 d后,将ST02菌剂用水按1∶100（V∶V）比例混匀（活菌数2×10⁷ CFU/g）制成菌液,用200 mL菌液进行浇灌;ST02处理7 d后,NaCl处理组用NaCl浇灌的同一时间,用正常水浇灌
	NaCl+ST02处理 NaCl+ST02 treatment	椒样薄荷茎段扦插7 d后,将ST02菌剂用水按1∶100（V∶V）比例混匀（活菌数2×10⁷ CFU/g）制成菌液,用200 mL菌液进行浇灌;ST02处理7 d后,用200 mmol/L NaCl溶液200 mL浇灌,每隔3 d进行1次NaCl处理,连续浇灌3次后用正常水浇灌
对照组Control		实验组用ST02菌液和NaCl处理的同时,用200 mL水浇灌

不同处理 Treatment	PGI1 SL	PGI2 FW	PGI3 Pn	PGI4 SPAD	PGI5 SOD	PGI6 POD	PGI7 CAT	PGCI
NaCl	-0.154	-0.155	-0.363	-0.371	-0.481	-0.391	-0.476	-2.391
ST02	0.099	0.056	0.014	0.040	0.146	0.212	0.176	0.743
NaCl+ST02	0.007	-0.024	0.011	0.037	-0.146	0.061	0.029	-0.025

不同处理 Treatment	PGI1 SL	PGI2 FW	PGI3 Pn	PGI4 SPAD	PGI5 SOD	PGI6 POD	PGI7 CAT	PGCI
NaCl	-0.154	-0.155	-0.363	-0.371	-0.481	-0.391	-0.476	-2.391
ST02	0.099	0.056	0.014	0.040	0.146	0.212	0.176	0.743
NaCl+ST02	0.007	-0.024	0.011	0.037	-0.146	0.061	0.029	-0.025

不同处理 Treatment	pH	EC/（μs·cm^-1）	CEC/（cmol·kg^-1）	OM/（g·kg^-1）	AN/（mg·kg^-1）	AP/（mg·kg^-1）	AK/（mg·kg^-1）
CK	7.56±0.09^b	182.80±6.02^c	16.13±2.01^b	20.04±3.5^a	99.70±21.69^a,b	26.84±9.02^a	140.22±17.64^a
NaCl	7.66±0.06^a	406.54±35.10^a	16.72±3.32^b	17.00±4.7^a	87.62±13.83^b	19.38±4.58^a	125.20±15.53^a
ST02	7.56±0.05^b	183.28±3.14^c	21.98±3.53^a	21.48±5.9^a	119.05±23.33^a,b	28.78±5.22^a	126.88±14.32^a
NaCl+ST02	7.68±0.06^a	335.98±49.07^b	18.28±0.98^b	22.50±6.0^a	128.60±30.96^a	27.18±9.73^a	125.85±14.74^a