Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (7): 278-286.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1238
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NIU Yu-hui1,2(), LI Xiang-rong1, WU Bei1,2, LI Hong-shan1,2, LI Dian-yu1,2, CHEN Lei1,2, WEI Suo-cheng2(), FENG Ruo-fei1()
Received:
2021-09-27
Online:
2022-07-26
Published:
2022-08-09
Contact:
WEI Suo-cheng,FENG Ruo-fei
E-mail:747449152@qq.com;weisc668@163.com;fengruofei@xbmu.edu.cn
NIU Yu-hui, LI Xiang-rong, WU Bei, LI Hong-shan, LI Dian-yu, CHEN Lei, WEI Suo-cheng, FENG Ruo-fei. Effects of Glucose and Sodium Butyrate on the rHSA Yield in CHO-rHSA Engineering Cell Line[J]. Biotechnology Bulletin, 2022, 38(7): 278-286.
补糖策略 Glucose supplement strategies/(g·L-1) | 补糖前葡萄糖含量 Glucose content in the medium before supplementing glucose/(g·L-1) | 补糖后葡萄糖含量 Glucose content in the medium after supplementing glucose /(g·L-1) |
---|---|---|
2.0→5.0 | 2.0 | 5.0 |
2.0→7.0 | 2.0 | 7.0 |
3.0→5.0 | 3.0 | 5.0 |
3.0→7.0 | 3.0 | 7.0 |
Table 1 Different strategies for supplementing glucose
补糖策略 Glucose supplement strategies/(g·L-1) | 补糖前葡萄糖含量 Glucose content in the medium before supplementing glucose/(g·L-1) | 补糖后葡萄糖含量 Glucose content in the medium after supplementing glucose /(g·L-1) |
---|---|---|
2.0→5.0 | 2.0 | 5.0 |
2.0→7.0 | 2.0 | 7.0 |
3.0→5.0 | 3.0 | 5.0 |
3.0→7.0 | 3.0 | 7.0 |
Fig.1 Effects of different glucose supplementation strategies on the growth of CHO-rHSA cells and the yield of rHSA A:Viable cell density. B:Viability. C:Glucose concentration. D:The productivity of rHSA was detected by ELISA(**P<0.01)
Fig.2 Effects of different concentrations of sodium butyrate on the growth of CHO-rHSA cells and the yield of rHSA A:Viable cell density. B:Viability. C:Glucose concentration. D:The protein expression of rHSA was detected by Western blot(Sd:Standard concentration 100 mg/L). E:The relative expression level of rHSA was detected by gray analysis. F:The productivity of rHSA was detected by ELISA(*P<0.05)
Fig.3 Effects of combined culture of glucose and sodium butyrate on the growth of CHO-rHSA cells and the yield of rHSA A:Viable cell density(①:3.0→5.0 g/L. ②:3.0→7.0 g/L). B:Viability. C:Glucose concentration. D:The protein expression of rHSA was detected by Western blot(Sd:standard concentration 100 mg/L). E:The relative expression level of rHSA was detected by gray analysis. F:The productivity of rHSA was detected by ELISA(***P<0.001)
Fig.4 Effect of adding time of sodium butyrate on the growth of CHO-rHSA cells and the yield of rHSA A:Viable cell density. B:Viability. C:Glucose concentration. D:The productivity of rHSA was detected by ELISA(*P<0.05)
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