Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (7): 51-61.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1524

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Expression Pattern Analysis of Histone Methyltransferase Under Drought Stress in Hybrid Wheat

CHEN Jia-min1,2(), LIU Yong-jie2, MA Jin-xiu2, LI Dan2, GONG Jie2, ZHAO Chang-ping2, GENG Hong-wei1(), GAO Shi-qing2()   

  1. 1. College of Agriculture,Xinjiang Agricultural University,Urumqi 830052
    2. Hybrid Wheat Research Institute,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097
  • Received:2021-12-08 Online:2022-07-26 Published:2022-08-09
  • Contact: GENG Hong-wei,GAO Shi-qing E-mail:cjmin2018@126.com;hw-geng@163.com;gshiq@126.com

Abstract:

The aim of this study is to screen the histone methyltransferases involved in the drought stress of wheat hybrids,and to provide functional markers for the improvement of stress resistance of two-line hybrid wheat. Screening of the total 175 TaHMT family genes in the wheat through the public databases on the website and the comparative genomics strategies,we obtained 8 candidate histone methyltransferases genes induced by drought stress(TaHMT21TaHMT24TaHMT31TaHMT42TaHMT49TaHMT105TaHMT143TaHMT157). The physical and chemical properties,protein structures,phylogenetic relationships,gene structures and conserved motifs of 8 TaHMTs were systematically analyzed via bioinformatics. Using drought treated hybrid wheat combinations(BS278*09YH91-5 and parental)as experimental materials,the candidate genes of 8 histone methyltransferase were verified via quantitative fluorescent PCR. It was found that their expressions were induced by drought stress,and the expression pattern presented mode of up-regulation first and then down-regulation with the drought stress time prolonged. The expressions of gene TaHMT21TaHMT24 and TaHMT42 accorded with the phenotypes of hybrid combinations,indicating that they may regulate the drought resistance of hybrids. These results provide important clues to deeply study the function of wheat TaHMT family genes in the drought resistance of hybrids as well as also provide excellent stress resistance gene resources for wheat molecular breeding.

Key words: wheat, hybrid, TaHMT, bioinformatics analysis, drought stress