Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (8): 159-166.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1393
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HE Li-na(), FENG Yuan, SHI Hui-min, YE Jian-ren()
Received:
2021-11-07
Online:
2022-08-26
Published:
2022-09-14
Contact:
YE Jian-ren
E-mail:helina0115@163.com;jrye@njfu.com.cn
HE Li-na, FENG Yuan, SHI Hui-min, YE Jian-ren. Screening and Identification of Endophytic Bacteria with Nematicidal Activity Against Bursaphelenchus xylophilus in Pinus massoniana[J]. Biotechnology Bulletin, 2022, 38(8): 159-166.
菌株编号 Strain No. | 校正死亡率Adjusted mortality/% | 消解率Decomposition rate/% | ||||||
---|---|---|---|---|---|---|---|---|
12 h | 24 h | 36 h | 48 h | 12 h | 24 h | 36 h | 48 h | |
NZM 13-11 | 92.2 | 97.2 | 100 | 100 | 85.0 | 86.6 | 90.0 | 100 |
Table 1 Nematicidal activities of the strain NZM13-11 against B. xylophilus
菌株编号 Strain No. | 校正死亡率Adjusted mortality/% | 消解率Decomposition rate/% | ||||||
---|---|---|---|---|---|---|---|---|
12 h | 24 h | 36 h | 48 h | 12 h | 24 h | 36 h | 48 h | |
NZM 13-11 | 92.2 | 97.2 | 100 | 100 | 85.0 | 86.6 | 90.0 | 100 |
Fig.2 Morphological observation of B.xylophilus treated with fermentation filtrate of NZM 13-11 strain A:CK;B:the contents of nematodes flowed out;C:the body of nematodes was broken and the contents were extravasated;D:the body wall of the nematodes was dissolved;E:the field of vision under 50 times microscopy after 24 h of treatment;F:the field of vision under 50 times microscopy after 36 h treatment
生理生化试验 Physiological and biochemical test | 鉴定结果 Results |
---|---|
葡萄糖 Glucose | + |
果糖 Fructose | + |
接触酶 Catalase | + |
氧化酶 Oxidase | + |
甲基红试验 Methyl red test | - |
VP(乙酰甲基甲醇)VP(Acetyl methyl methanol) | - |
淀粉水解 Starch hydrolysis | - |
硝酸盐还原 Nitrate reduction | + |
亚硝酸盐还原 Nitrite reduction | + |
反硝化 Denitrification | + |
明胶水解 Elatin hydrolysis | + |
精氨酸双水解酶 Arginine dihydrolase | + |
Table 2 Physiological and biochemical characteristics of strain NZM13-11
生理生化试验 Physiological and biochemical test | 鉴定结果 Results |
---|---|
葡萄糖 Glucose | + |
果糖 Fructose | + |
接触酶 Catalase | + |
氧化酶 Oxidase | + |
甲基红试验 Methyl red test | - |
VP(乙酰甲基甲醇)VP(Acetyl methyl methanol) | - |
淀粉水解 Starch hydrolysis | - |
硝酸盐还原 Nitrate reduction | + |
亚硝酸盐还原 Nitrite reduction | + |
反硝化 Denitrification | + |
明胶水解 Elatin hydrolysis | + |
精氨酸双水解酶 Arginine dihydrolase | + |
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