Effects of Pseudomonas sp. PR3,a Pyrene-degrading Bacterium with Plant Growth-promoting Properties,on Rice Growth Under Pyrene Stress

doi:10.13560/j.cnki.biotech.bull.1985.2022-0539

Abstract

Abstract:

The accumulation of polycyclic aromatic hydrocarbons（PAHs）in farmland not only seriously hinder crop growth,but also increase food security risks. Screening the microorganisms with both the functions of promoting plant growth and degrading pollutants is an effective means to solving the above problems. Strain PR3 was isolated from the root surface of plants growing near the oil field,which had the ability of degrading pyrene,and also showed the properties of promoting plant growth like phosphorus dissolution,indole acetic acid and siderophore production. The results of 16S rDNA sequence analysis showed that the strain was classified as Pseudomonas sp. After growing in PMM medium containing 20 mg/L pyrene for 14 d,the degradation rate of pyrene by strain PR3 reached 94%. The degradation rates of naphthalene（50 mg/L）,phenanthrene（50 mg/L）and benzo（a）pyrene（10 mg/L）at 14 d were 92%,84% and 47%,respectively. Furthermore,the maximum dissolved phosphorus amount of strain PR3 was 756.25 mg/L in 7 d,the synthesis amount of IAA in 2 d was up to 14.46 mg/L,and the synthetic siderophore activity unit in 4 d reached 58.53%. Greenhouse pot experiments under different pyrene contamination concentration demonstrated that the inoculation of strain PR3 significantly improved the growth of rice and increased the degradation of pyrene near rhizosphere,and the removal rate reached 72.02%-86.22%. Meanwhile,the pyrene contents in the roots and shoots decreased by 21.81%-53.01% and 49.81%-57.17%,respectively. These results indicated that strain PR3 could contribute to the ecological remediation of pyrene-contaminated soil and reduce the risk of pyrene exposure to crops.

Key words: pyrene-degrading bacteria, plant growth-promoting properties, rice, soil remediation, oxidative stress

GAO Xiao-rong, DING Yao, LV Jun. Effects of Pseudomonas sp. PR3,a Pyrene-degrading Bacterium with Plant Growth-promoting Properties,on Rice Growth Under Pyrene Stress[J]. Biotechnology Bulletin, 2022, 38(9): 226-236.

Figures/Tables 8

References 42

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菌株编号 Strain No.	溶磷量 Phosphate solubilisation/（mg·L^-1）	IAA生成量 IAA production/（mg·L^-1）	铁载体显色圈 Siderophore color circle/（D·d^-1）	铁载体活性单位 Siderophore activity unit/%
PR1	736.77±20.30	11.38±0.33	2.76±0.34	57.67±1.21
PR2	702.25±28.60	2.40±0.81	5.17±1.02	62.57±3.20
PR3	756.25±15.01	14.46±0.20	2.53±0.52	58.53±3.62
PR4	893.46±25.38	9.24±0.84	1.05±0.45	38.47±3.15
PR5	842.80±36.84	9.50±0.88	1.25±0.11	31.24±1.55
PR6	762.09±24.75	4.05±0.61	1.02±0.28	25.67±1.87

菌株编号 Strain No.	溶磷量 Phosphate solubilisation/（mg·L^-1）	IAA生成量 IAA production/（mg·L^-1）	铁载体显色圈 Siderophore color circle/（D·d^-1）	铁载体活性单位 Siderophore activity unit/%
PR1	736.77±20.30	11.38±0.33	2.76±0.34	57.67±1.21
PR2	702.25±28.60	2.40±0.81	5.17±1.02	62.57±3.20
PR3	756.25±15.01	14.46±0.20	2.53±0.52	58.53±3.62
PR4	893.46±25.38	9.24±0.84	1.05±0.45	38.47±3.15
PR5	842.80±36.84	9.50±0.88	1.25±0.11	31.24±1.55
PR6	762.09±24.75	4.05±0.61	1.02±0.28	25.67±1.87

PAHs	初始浓度 Initial concentration/ （mg·L^-1）	降解时间 Degrading time/d	PAHs降解率 Degradation rate of PAHs/%
萘 Nap	50	7	91.56±1.35
萘 Nap	50	14	92.45±1.04
菲Phe	50	7	52.39±5.78
菲Phe	50	14	84.70±1.54
苯并（a）芘 Bap	10	7	46.37±7.48
苯并（a）芘 Bap	10	14	47.16±2.66

PAHs	初始浓度 Initial concentration/ （mg·L^-1）	降解时间 Degrading time/d	PAHs降解率 Degradation rate of PAHs/%
萘 Nap	50	7	91.56±1.35
萘 Nap	50	14	92.45±1.04
菲Phe	50	7	52.39±5.78
菲Phe	50	14	84.70±1.54
苯并（a）芘 Bap	10	7	46.37±7.48
苯并（a）芘 Bap	10	14	47.16±2.66

处理 Treatment		地上部 Shoot		根 Root
处理 Treatment		鲜重Fresh weight/（mg·pot^-1）	干重Dry weight/（mg·pot^-1）	鲜重Fresh weight/（mg·pot^-1）	干重Dry weight/（mg·pot^-1）
S0	CP	2861.76±16.80 c	774.40±15.12 b	1775.95±39.67 bc	303.60±17.08 c
	CPR	3536.13±103.75 ab	860.93±20.17 a	2456.30±162.76 a	368.13±18.42 a
S1	CP	3081.10±71.56 bc	792.00±20.61 b	1532.85±154.78 cd	287.47±18.87 cd
	CPR	3380.85±70.78 b	871.20±21.78 a	1894.20±60.74 b	334.40±7.21 b
S2	CP	2681.43±75.64 c	630.67±17.54 c	1611.13±154.24 c	262.90±14.55 d
	CPR	3788.40±82.44 a	854.70±31.33 a	2323.75±103.45 a	373.63±9.61 a
S3	CP	2069.10±105.98 d	493.53±12.12 d	1155.00±113.43 d	255.93±16.66 d
	CPR	3513.95±119.01 ab	838.57±30.83 ab	2113.10±49.78 a	346.13±14.77 ab