Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (9): 215-225.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1507

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Optimization of Producing Cellulase by Aspergillus fumigatus A-16 and Its Enzymatic Properties

ZHANG Kai-ping1(), LIU Yan-li1, TU Mian-liang1, LI Ji-wei1, WU Wen-biao2()   

  1. 1. Baise University,Baise 533000
    2. College of Food Science,Southwest University,Chongqing 400716
  • Received:2021-11-28 Online:2022-09-26 Published:2022-10-11
  • Contact: WU Wen-biao E-mail:1453571341@qq.com;wbwu2006@sina.com

Abstract:

The aim of this work is to screen efficient straw degradable strains and study their process of cellulase and characteristics. A cellulase-producing strain was isolated from soil under decayed wood using the Congo red staining test,and then was identified through morphological characteristics and 18S rDNA sequence comparisons. The condition of enzyme production was determined by single factor experiment and response surface experiments,and the stability of cellulase was investigated. As results,the isolated strain was named Aspergillus fumigatusAspergillus fumigatus A-16). The response surface test showed that the optimal process parameters of producing cellulase were as follows:straw powder 7 g/100 mL,pH 6.0,fermentation temperature 65℃,and fermentation time 5 d. Under this optimal fermentation conditions,the carboxymethyl cellulase(CACase)and filter paper activities(FPA)was 2 954.76 U/mL and 1 086.37 U/mL,and the total enzyme activity was 26.4% higher than that before optimization. The suitable reaction temperature was 70℃ and pH 6.0 of CMCase and FPA. The relative enzyme activity of the enzyme retained at over 80% after being heated for 90 min at 80℃,indicating that it had a good thermal stability. Meanwhile,the relative enzyme activity of the enzyme was stable in the pH 5.0-7.0 and could be remained > 70% after 1 d. This study may provide basic support for exploiting clean energy and food-grade glucose resource by using cellulose-rich biomass as a raw material.

Key words: Aspergillus fumigatus, cellulase, condition of producing enzyme, enzymatic properties