Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (1): 157-165.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0560

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Functional Analysis of ACOL8 Gene in the Ethylene Synthesis and Response in Arabidopsis thaliana

LIN Rong1(), ZHENG Yue-ping2(), XU Xue-zhen2, LI Dan-dan2, ZHENG Zhi-fu1,2   

  1. 1. College of Forestry and Biotechnology,Zhejiang Agriculture and Forestry University,Hangzhou 311300
    2. College of Advanced Agricultural Sciences,Zhejiang Agriculture and Forestry University,Hangzhou 311300
  • Received:2022-05-09 Online:2023-01-26 Published:2023-02-02
  • Contact: ZHENG Yue-ping E-mail:lynerlin@163.com;zyp860819@126.com

Abstract:

The plant hormone ethylene plays pivotal roles in various physiological and biochemical processes. However, the mechanisms controlling its synthesis in certain tissues and organs are not completely understood. There are 12 ACC oxidase homologs(ACO-like homolog, ACOL)of unknown functions in Arabidopsis thaliana. The loss-of-function mutants of ACOL8 gene were constructed using site-directed gene editing technology. It was found that the mutations of this gene attenuated the classical ethylene triple response. Compared with wild type, the mutants showed significant increases in the length of hypocotyls and primary roots of etiolated seedlings, which was consistent with the observation that the mutants had decreased sensitivity to exogenous ACC. Additionally, it was evident that the expression of this gene was subjected to positive feedback regulation of ethylene signaling, as exemplified by the finding that overexpression of EIN3 increased the expression of ACOL8 gene, whereas the etr1-3 muation exerted the opposite effect. Furthermore, the mutation of ACOL8 gene did not appear to affect the growth of A. thaliana under normal conditions, whereas the root-shoot ratio of the mutants decreased significantly under salt stress conditions, suggesting its involvement in plant salt stress response. Collectively, these results indicate that ACOL8 may function as an ACC oxidase to medaite ethylene synthesis and response.

Key words: ACC oxidase-like homolog, CRISPR/Cas9 gene editing technology, ethylene synthesis, salt tolerance, Arabidopsis thaliana