Whole-Genome Sequencing Identification of Phosphate-solubilizing Bacteria PSB-R and Analysis of Its Phosphate-solubilizing Properties

doi:10.13560/j.cnki.biotech.bull.1985.2022-0448

Abstract

Abstract:

The phosphate-solubilizing bacterium PSB-R was identified as Serratia marcescens by physiological and biochemical indicators combined with molecular biology technology. Second-generation sequencing platform Illumina NovaSeq PE150 was used to have the whole genome of PSB-R sequenced, and to analyze and predict the composition of genes related to phosphate-solubilizing ability and other plant growth-promoting genes. The response surface optimization test showed that the maximum phosphorus dissolving capacity of PSB-R was 805.199 mg/L. After 10 generations of continuous culture, the phosphorus dissolving capacity was stable and had the ability to dissolve a variety of insoluble phosphates. This study provides a genomic data basis for the further study of the mechanism of phosphate solubilization by phosphate solubilizing bacteria, and confirms that there is a potential for PSB-R to be applied to bacterial fertilizer, which provides a research basis for the subsequent development of phosphate-solubilizing bacterial fertilizer.

Key words: phosphate-solubilizing bacteria, Serratia marcescens, response surface optimization, whole genome sequencing

WANG Shuai, LV Hong-rui, ZHANG Hao, WU Zhan-wen, XIAO Cui-hong, SUN Dong-mei. Whole-Genome Sequencing Identification of Phosphate-solubilizing Bacteria PSB-R and Analysis of Its Phosphate-solubilizing Properties[J]. Biotechnology Bulletin, 2023, 39(1): 274-283.

Figures/Tables 9

References 27

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项目Item	数据Data
Scaffold数量 Number of Scaffold	16
Contig数量 Number of Contig	16
（G+C）/%	59.75
基因组大小Genome size/bp	5 061 510
预测基因数量 Number of genes	4 742
基因总长度Total gene length/bp	4 393 551

项目Item	数据Data
Scaffold数量 Number of Scaffold	16
Contig数量 Number of Contig	16
（G+C）/%	59.75
基因组大小Genome size/bp	5 061 510
预测基因数量 Number of genes	4 742
基因总长度Total gene length/bp	4 393 551

基因号码Gene code	基因名称Gene name	产物名称 Product name	生物活性 Activity
PSB.R_GM000437	gcd	PQQ依赖型葡萄糖脱氢酶 Quinoprotein glucose dehydrogenase	葡萄糖酸合成 Gluconic acid synthesis
PSB.R GM001125	ppq B	PQQ合成蛋白B Cofactor PQQ biosynthesis protein B
PSB.R GM001126	ppq C	PQQ合成蛋白C Cofactor PQQ biosynthesis protein C
PSB.R GM001127	ppq D	PQQ合成蛋白D Cofactor PQQ biosynthesis protein D
PSB.R GM001128	ppq E	PQQ合成蛋白E Cofactor PQQ biosynthesis protein E
PSB.R GM001129	ppq F	PQQ合成蛋白F Cofactor PQQ biosynthesis protein F
PSB.R_GM000385	ent A	2,3-二氢-2,3-二羟基苯甲酸脱氢酶 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase	铁载体产生 Siderophore production
PSB.R_GM003128	ent C	异分支酸合成酶C Isochorismate synthase C
PSB.R_GM003126	ent B	肠杆菌素合成亚基B Enterobactin synthase subunit B
PSB.R_GM003127	ent E	肠杆菌素合成亚基E Enterobactin synthase subunit E
PSB.R_GM003130	ent F	肠杆菌素合成亚基F Enterobactin synthase subunit F
PSB.R_GM003129	ent S	肠杆菌素（铁载体）外排蛋白 Enterobactin（siderophore）exporter
PSB.R_GM002907	exb B	生物聚合物转运蛋白B Biopolymer transport protein B
PSB.R_GM002910	exb D	生物聚合物转运蛋白D Biopolymer transport protein D
PSB.R_GM004736	bfr	细菌铁蛋白 Bacterioferritin
PSB.R_GM000070	ipd C	吲哚丙酮酸脱羧酶 Indolepyruvate decarboxylase	IAA产生 IAA production
PSB.R_GM000012	chi	几丁质酶 Chitinase	几丁质酶产生 Chitinase production
PSB.R_GM003955	ppx	外切聚磷酸酶 Exopolyphosphatase	磷酸盐降解酶 Phosphate degrading enzyme
PSB.R_GM003047	ppa	无机焦磷酸酶 Inorganic pyrophosphatase
PSB.R_GM001406	phn X	磷酸乙醛水解酶 Phosphonoacetaldehyde hydrolase
PSB.R_GM002025	pho A	碱性磷酸酶 Alkaline phosphatase

基因号码Gene code	基因名称Gene name	产物名称 Product name	生物活性 Activity
PSB.R_GM000437	gcd	PQQ依赖型葡萄糖脱氢酶 Quinoprotein glucose dehydrogenase	葡萄糖酸合成 Gluconic acid synthesis
PSB.R GM001125	ppq B	PQQ合成蛋白B Cofactor PQQ biosynthesis protein B
PSB.R GM001126	ppq C	PQQ合成蛋白C Cofactor PQQ biosynthesis protein C
PSB.R GM001127	ppq D	PQQ合成蛋白D Cofactor PQQ biosynthesis protein D
PSB.R GM001128	ppq E	PQQ合成蛋白E Cofactor PQQ biosynthesis protein E
PSB.R GM001129	ppq F	PQQ合成蛋白F Cofactor PQQ biosynthesis protein F
PSB.R_GM000385	ent A	2,3-二氢-2,3-二羟基苯甲酸脱氢酶 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase	铁载体产生 Siderophore production
PSB.R_GM003128	ent C	异分支酸合成酶C Isochorismate synthase C
PSB.R_GM003126	ent B	肠杆菌素合成亚基B Enterobactin synthase subunit B
PSB.R_GM003127	ent E	肠杆菌素合成亚基E Enterobactin synthase subunit E
PSB.R_GM003130	ent F	肠杆菌素合成亚基F Enterobactin synthase subunit F
PSB.R_GM003129	ent S	肠杆菌素（铁载体）外排蛋白 Enterobactin（siderophore）exporter
PSB.R_GM002907	exb B	生物聚合物转运蛋白B Biopolymer transport protein B
PSB.R_GM002910	exb D	生物聚合物转运蛋白D Biopolymer transport protein D
PSB.R_GM004736	bfr	细菌铁蛋白 Bacterioferritin
PSB.R_GM000070	ipd C	吲哚丙酮酸脱羧酶 Indolepyruvate decarboxylase	IAA产生 IAA production
PSB.R_GM000012	chi	几丁质酶 Chitinase	几丁质酶产生 Chitinase production
PSB.R_GM003955	ppx	外切聚磷酸酶 Exopolyphosphatase	磷酸盐降解酶 Phosphate degrading enzyme
PSB.R_GM003047	ppa	无机焦磷酸酶 Inorganic pyrophosphatase
PSB.R_GM001406	phn X	磷酸乙醛水解酶 Phosphonoacetaldehyde hydrolase
PSB.R_GM002025	pho A	碱性磷酸酶 Alkaline phosphatase