Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (3): 290-300.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0919

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Genome Sequencing and Bioinformatics Analysis of Gelidibacter sp. PG-2

ZHANG Zhi-xia(), LI Tian-pei, ZENG Hong, ZHU Xi-xian, YANG Tian-xiong, MA Si-nan, HUANG Lei()   

  1. College of Chemistry and Chemical Engineering,Tianjin University of Technology,Tianjin 300384
  • Received:2022-07-24 Online:2023-03-26 Published:2023-04-10

Abstract:

Gelidibacter sp. PG-2 is a carotenoid-producing strain, and genome-wide sequencing was conducted in order to further study its carotenoid-producing mechanisms. After the carotenoids were extracted from PG-2, qualitative analysis was performed by LC-MS/MS, the content was calculated by liquid UV full wavelength scanning, and the metabolic pathways of carotenoid-producing in this strain were predicted based on the results of whole genome sequencing. The results showed that the carotenoids produced by PG-2 were zeaxanthin with a content of 185.81 μg/g of dry weight, the whole genome size of PG-2 was 3 850 413 bp, the GC content was 37.91%, the total rRNA was 5, the total tRNA was 37, and the sRNA was 19, which were annotated in COG, GO and KEGG databases to genes 3 401, 1 797 and 1 495, respectively. The strain PG-2 contained three core genes associated with carotenoid production, crtI, crtZ, and lcyB, and their metabolic pathways were predicted. GenBank accession number JAMJTV000000000 was obtained after Genome Framework Sequencing Data submitted to NCBI. The above results demonstrate that the strain PG-2 has the ability to produce carotenoids, and it is speculated that its carotenoid production is related to the genes crtI, crtZ and lcyB.

Key words: Gelidibacter sp., whole genome sequencing, liquid UV full wavelength scans, carotenoids