Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (6): 259-273.doi: 10.13560/j.cnki.biotech.bull.1985.2022-1316

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Transcriptome Analysis of Interaction Between Gossypium barbadense and Fusarium oxysporum f. sp. vasinfectum

YANG Yang1(), ZHU Jin-cheng1, LOU Hui1, HAN Ze-gang2(), ZHANG Wei1()   

  1. 1. Key Laboratory of Oasis Eco-agriculture of Xinjiang Production and Construction Corps, College of Agronomy, Shihezi University, Shihezi 832003
    2. Advanced Seed Institution, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058
  • Received:2022-10-26 Online:2023-06-26 Published:2023-07-07
  • Contact: HAN Ze-gang, ZHANG Wei E-mail:1977277872@qq.com;zeganghan@zju.edu.cn;zhw_agr@shzu.edu.cn

Abstract:

Cotton Fusarium wilt is a common disease in cotton production, especially very seriously in island cotton(Gossypium barbadense), affecting its yield and quality, which pose a great threat to the development of island cotton industry. To understand the molecular mechanism of the interaction between cotton and Fusarium oxysporum, RNA-seq sequencing technology was applied to analyze the gene expression characteristics of the interaction between Fusarium oxysporum and cotton using the resistant and susceptible cotton root tissues and pathogenic bodies infected by Fusarium oxysporum for 48 h as materials. The results showed that 15 218 and 9 358 differentially expressed genes were detected in resistant and susceptible cotton varieties respectively, 3 708 and 3 656 differentially expressed genes were identified in Fusarium oxysporum after infecting the resistant and susceptible cotton varieties. Through GO enrichment analysis, we found that the main processes in cotton after interaction were oxidative stress, auxin-activated signaling pathway, response to stimulus, response to injury and transcription factor activity. KEGG pathways were significantly enriched in endocytosis, plant hormone signal transduction, amino acid biosynthesis, carbon metabolism, plant-pathogen interactions, phenylpropane biosynthesis and other metabolic pathways. Disease-resistant cultivars were significantly up-regulated in responses to stimulation and injury. In the Fusarium oxysporum after interaction, GO enrichment analysis found that differentially expressed genes were mostly involved in membrane components, catalytic activity regulation, ATP binding etc. KEGG was significantly enriched in peroxisome, mitogen-activated protein kinase signaling pathways, valine, leucine and L-Isoleucine degradation, glycine, serine and threonine metabolism, carbon metabolism, amino acid biosynthesis, starch and sucrose metabolism and other metabolic pathways. This study provids an abundant gene resources for studying the responses of cotton to Fusarium wilt and the pathogenicity of F. oxysporum, and laid a foundation for an in-depth analysis of the mechanism of interaction between Fusarium oxysporum and cotton.

Key words: Fusarium oxysporum, cotton, Fusarium wilt, plant-pathogen interactions, RNA-seq, differentially expressed genes