Research Progress in the Structure of Tailed Bacteriophage and Its Receptors

doi:10.13560/j.cnki.biotech.bull.1985.2022-1329

Abstract

Abstract:

With the emergence and rapid spread of “super drug-resistant” bacteria, phages have become a research hot spot for antibiotic alternatives and a new way to solve the problem of antibiotic resistance and promote the healthy development of the farming industry. The key to the therapeutic role of phages is their ability of specifically lysing their host bacteria, while the specificity of phage lysis of bacteria depends on the recognition and adsorption of phage receptor-binding proteins to the receptor. Tailed bacteriophages use a broad range of receptor-binding proteins, such as tail fiber, tail spikes and the central tail spike, to target their cognate bacterial cell surface receptors lipopolysaccharide, outer membrane protein, capsule, flagella and pili, etc.，and finally the bacteria are lysed. In the present review, we systematically summarized the research advances in the types and structures of tailed bacteriophage and its receptors. We also discussed the selection strategies of phage therapeutic agents based on the research foundation of phage-host interaction mechanism. It is aimed to provide a solid theoretical foundation for the further study of the mechanism of interaction between phages and their host bacteria, modification of phages and creation of phage biocidal agents.

Key words: tailed bacteriophage, structure, receptor-binding protein, receptor

LI Tuo, LI Long-ping, QU Lei. Research Progress in the Structure of Tailed Bacteriophage and Its Receptors[J]. Biotechnology Bulletin, 2023, 39(6): 88-101.

Figures/Tables 3

References 130

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噬菌体类型 Phage type	特征 Features	噬菌体 Phage species	宿主细菌 Host bacterium	噬菌体受体结合蛋白 Receptor-binding protein	受体 Receptor	参考文献 References
肌尾科噬菌体 Myoviridae	具有长的可收缩尾巴 Long contractile tail	T2、T4	大肠杆菌Escherichia coli	尾部纤维	OmpC、OmpF	[22,38]
		SPN3US	沙门氏菌Salmonella enterica	尾部纤维	鞭毛	[39]
		MAM1	沙雷氏菌属、克鲁维菌属 Enterobacterial genera Serratia and Kluyvera	尾钉	荚膜	[40]
		Det7	沙门氏菌S. enterica	尾钉	脂多糖	[15]
		JG004	铜绿假单胞菌 Pseudomonas aeruginosa	-	脂多糖	[41]
长尾科噬菌体 Siphoviridae	具有长的不可收缩尾巴 Long non-contractile tail	T5	大肠杆菌E. coli	尾部纤维	脂多糖O抗原	[42]
		SSU5	沙门氏菌S. enterica	尾部纤维	脂多糖核心多糖	[43]
		λ、TLS、H8、phi2013	大肠杆菌 E. coli	尾部纤维	LamB、TolC、FepA、FhuA	[44⇓⇓-47]
		iEPS5	沙门氏菌S. enterica	尾部纤维	鞭毛	[48]
		DMS3	铜绿假单胞菌P. aeruginosa	尾部纤维	菌毛	[49]
		Vi-II	沙门氏菌S. enterica	尾钉	荚膜	[18]
		9NA	沙门氏菌S. enterica	尾钉	脂多糖	[33]
		phiChi13、phiCbK	Caulobacter crescentus	衣壳	鞭毛	[50]
		SPC35	沙门氏菌S. enterica	尾部纤维	BtuB	[51]
		SPN7C、9C、10H、12C、14、17T、18	沙门氏菌S. enterica	-	鞭毛Flagella、BtuB、脂多糖O抗原	[52]
短尾科噬菌体 Podoviridae	具有短的可收缩尾巴 Short contractile tail	T3、T4、T7	大肠杆菌E. coli	尾部纤维	LPS, OmpC	[6,53 -54]
		Bp7	大肠杆菌E. coli	-	LamB、OmpC、HepI	[55]
		Yep-phi	鼠疫耶尔氏菌Yersinia pestis	尾部纤维	Ail、OmpF	[56]
		MPK7	铜绿假单胞菌P. aeruginosa	尾部纤维	IV型菌毛	[57]
		phiK1-K5	大肠杆菌E. coli	尾钉	荚膜	[58]
		P22	沙门氏菌S. enterica	尾钉	脂多糖O抗原	[59]
		Sf6	志贺氏杆菌Shigella flexneri	尾钉	OmpA	[60]

噬菌体类型 Phage type	特征 Features	噬菌体 Phage species	宿主细菌 Host bacterium	噬菌体受体结合蛋白 Receptor-binding protein	受体 Receptor	参考文献 References
肌尾科噬菌体 Myoviridae	具有长的可收缩尾巴 Long contractile tail	T2、T4	大肠杆菌Escherichia coli	尾部纤维	OmpC、OmpF	[22,38]
		SPN3US	沙门氏菌Salmonella enterica	尾部纤维	鞭毛	[39]
		MAM1	沙雷氏菌属、克鲁维菌属 Enterobacterial genera Serratia and Kluyvera	尾钉	荚膜	[40]
		Det7	沙门氏菌S. enterica	尾钉	脂多糖	[15]
		JG004	铜绿假单胞菌 Pseudomonas aeruginosa	-	脂多糖	[41]
长尾科噬菌体 Siphoviridae	具有长的不可收缩尾巴 Long non-contractile tail	T5	大肠杆菌E. coli	尾部纤维	脂多糖O抗原	[42]
		SSU5	沙门氏菌S. enterica	尾部纤维	脂多糖核心多糖	[43]
		λ、TLS、H8、phi2013	大肠杆菌 E. coli	尾部纤维	LamB、TolC、FepA、FhuA	[44⇓⇓-47]
		iEPS5	沙门氏菌S. enterica	尾部纤维	鞭毛	[48]
		DMS3	铜绿假单胞菌P. aeruginosa	尾部纤维	菌毛	[49]
		Vi-II	沙门氏菌S. enterica	尾钉	荚膜	[18]
		9NA	沙门氏菌S. enterica	尾钉	脂多糖	[33]
		phiChi13、phiCbK	Caulobacter crescentus	衣壳	鞭毛	[50]
		SPC35	沙门氏菌S. enterica	尾部纤维	BtuB	[51]
		SPN7C、9C、10H、12C、14、17T、18	沙门氏菌S. enterica	-	鞭毛Flagella、BtuB、脂多糖O抗原	[52]
短尾科噬菌体 Podoviridae	具有短的可收缩尾巴 Short contractile tail	T3、T4、T7	大肠杆菌E. coli	尾部纤维	LPS, OmpC	[6,53 -54]
		Bp7	大肠杆菌E. coli	-	LamB、OmpC、HepI	[55]
		Yep-phi	鼠疫耶尔氏菌Yersinia pestis	尾部纤维	Ail、OmpF	[56]
		MPK7	铜绿假单胞菌P. aeruginosa	尾部纤维	IV型菌毛	[57]
		phiK1-K5	大肠杆菌E. coli	尾钉	荚膜	[58]
		P22	沙门氏菌S. enterica	尾钉	脂多糖O抗原	[59]
		Sf6	志贺氏杆菌Shigella flexneri	尾钉	OmpA	[60]

噬菌体类型 Phage type	特征 Features	噬菌体 Phage species	宿主细菌 Host bacterium	噬菌体受体结合蛋白 Receptor-binding protein	受体 Receptor	参考文献 References
肌尾科噬菌体 Myoviridae	具有长的可收缩尾巴 Long contractile tail	γ	炭疽杆菌Bacillus anthracis	尾部纤维	GamR	[61]
		A511	李斯特菌Listeria monocytogenes	尾部纤维	磷壁酸，肽聚糖	[62]
		φ812、φK	金黄色葡萄球菌 Staphylococcus aureus	-	磷壁酸	[63]
长尾科噬菌体 Siphoviridae	具有长的不可收缩尾巴 Long non-contractile tail	IL-H	德氏乳酸杆菌 Lactobacillus delbrueckii	尾部纤维	磷壁酸	[64]
		SPP1	枯草芽孢杆菌B. subtilis	尾钉	YueB	[65]
		φSLT	金黄色葡萄球菌 Staphylococcus aureus	中央尾尖	磷壁酸	[66]
短尾科噬菌体 Podoviridae	具有短的可收缩尾巴 Short contractile tail	phi29	枯草芽孢杆菌B. subtilis	尾钉	磷壁酸	[35]
短尾科噬菌体 Podoviridae	具有短的可收缩尾巴 Short contractile tail	P2	乳酸乳球菌Lactococcus lactis	尾部纤维	细胞壁	[67]

噬菌体类型 Phage type	特征 Features	噬菌体 Phage species	宿主细菌 Host bacterium	噬菌体受体结合蛋白 Receptor-binding protein	受体 Receptor	参考文献 References
肌尾科噬菌体 Myoviridae	具有长的可收缩尾巴 Long contractile tail	γ	炭疽杆菌Bacillus anthracis	尾部纤维	GamR	[61]
		A511	李斯特菌Listeria monocytogenes	尾部纤维	磷壁酸，肽聚糖	[62]
		φ812、φK	金黄色葡萄球菌 Staphylococcus aureus	-	磷壁酸	[63]
长尾科噬菌体 Siphoviridae	具有长的不可收缩尾巴 Long non-contractile tail	IL-H	德氏乳酸杆菌 Lactobacillus delbrueckii	尾部纤维	磷壁酸	[64]
		SPP1	枯草芽孢杆菌B. subtilis	尾钉	YueB	[65]
		φSLT	金黄色葡萄球菌 Staphylococcus aureus	中央尾尖	磷壁酸	[66]
短尾科噬菌体 Podoviridae	具有短的可收缩尾巴 Short contractile tail	phi29	枯草芽孢杆菌B. subtilis	尾钉	磷壁酸	[35]
短尾科噬菌体 Podoviridae	具有短的可收缩尾巴 Short contractile tail	P2	乳酸乳球菌Lactococcus lactis	尾部纤维	细胞壁	[67]