Screening of a Heterotrophic Nitrifying-aerobic Denitrifying Bacterium and Its Nitrogen Transforming Characterization

doi:10.13560/j.cnki.biotech.bull.1985.2023-0614

Abstract

Abstract:

The excessive nitrogen in aquaculture easily causes the deterioration of water quality and threatens the growth and reproduction of aquatic animals, thus a safe and efficient nitrogen removal method is urgently needed. An ammonia-degrading bacterium was selected from the sludge at the bottom of an aquaculture pond in Fujian province. After the strain identification, the adaptive domestication was carried out. The effects of different conditions on the growth of the strain and its ammonia-nitrogen degradation were studied. Subsequently, nitrite was used as the only nitrogen source to detect the aerobic denitrification characteristics of the strains. The strain was identified as Bacillus aryabhattai by 16S rRNA and named as JN01. After acclimation, the growth of the strain and removal rate of ammonia nitrogen increased at varied degrees, and the removal rate of nitrogen was up to 87.29% with the initial NH₄⁺-N concentration was 200 mg/L. The results of single factor experiment showed that the degradation rate of ammonia nitrogen of the strain reached 92.78% when NH₄⁺-N concentration was 200 mg/L, sodium succinate as carbon source, carbon to nitrogen ratio was 15∶1, pH 7.5 and culture temperature was 30℃. Under the condition that nitrite nitrogen was the only nitrogen source, strain JN01 also performed aerobic denitrification, and the degradation rate of NO₂^--N was 82.30%. In conclusion, B. aryabhattai JN01 has promising heterotrophic nitrification and aerobic denitrification characteristics, and has the potential to concurrently solve the excessive ammonia nitrogen and nitrite in aquaculture wastewater.

Key words: Bacillus aryabhattai, heterotrophic nitrification, aerobic denitrification, ammonia nitrogen, nitrite nitrogen

JIANG Hui-hui, WANG Qiang, FU Wei-lai, RAO Zhi-ming, ZHANG Xian. Screening of a Heterotrophic Nitrifying-aerobic Denitrifying Bacterium and Its Nitrogen Transforming Characterization[J]. Biotechnology Bulletin, 2023, 39(12): 250-260.

Figures/Tables 11

Fig. 1 Nitrogen metabolism pathways of HNAD microorganisms

Fig. 2 Growth curves（A）and NH4+-N removal efficiencies（B）of different strains

Fig. 3 Phylogenetic tree of the strain JN01

Fig. 4 Variation of OD600 nm（A）and NH4+-N removal efficiency（B）before and after the domestication of B. aryabhattai JN01

Fig. 5 Effects of N concentration on the growth characteristics OD600 nm（A）and NH4+-N removal efficiencies（B）of B. aryabhattai JN01

Fig. 6 Effects of different carbon sources on the growth characteristics OD600 nm（A）and NH4+-N removal efficiencies（B）of B. aryabhattai JN01

Fig. 7 Effects of C/N on the growth performance OD600 nm（A）and NH4+-N removal efficiencies（B）of B. aryabhattai JN01

Fig. 8 Effects of pH on the growth performance OD600 nm（A）and NH4+-N removal efficiencies（B）of B. aryabhattai JN01

Fig. 9 Effects of temperature on the growth performance OD600 nm（A）and NH4+-N removal efficiencies（B）of B. aryabhattai JN01

Fig. 10 Growth characteristics of strain JN01 and trends of NH4+-N, NO3--N, NO2--N, TN with initial NH4+-N (A) or initial NO2--N (B) as sole nitrogen source

Table 1 Removal efficiencies of NH4+-N, NO3--N, NO2--N and TN by HNAD microorganisms

菌株名Strain	来源Source	NH₄⁺-N去除率NH₄⁺-N removal efficiency/%	NO₃^--N去除率NO₃^--N removal efficiency/%	NO₂^--N去除率NO₂^--N removal efficiency/%	总氮去除率Total nitrogen removal effciency/%	参考文献Reference
芽孢杆菌属Bacillus sp.
B. aryabhattai JN01	水产养殖池底污泥	92.78	/	82.30	80.73	本文
B. Subtilis Ab03	鱼虾养殖池塘	91.67	/	/	89.01	[10]
B. thuringiensis WXN-23	猪场猪皮饲料滤液	86.74	90.74	100.00	82.12	[11]
B. cereus GS-5	生物膜	87.10-93.10	69.40-88.40	/	/	[17]
B. litoralis N31	养殖水体	86.30	89.40	89.30	/	[18]
B. simplex H-b	土壤	82.16	67.29	78.69	/	[19]
其他种属微生物 Other species of microorganisms
H.venusta TJPU05	运城盐湖	86.12	95.68	100.00	84.57	[6]
P. alcaliphila	冬季水浸稻田土壤	100.00	100.00	100.00	/	[7]
Enterococcus sp. XH1	黄河三角洲海水	78.90	68.20	/	78.80	[12]
Acinetobacter johnsonii sp. N26	羊粪堆肥	95.50	93.60	/	/	[15]
Pseudomonas sp.GZWN4	养殖水体	98.62	82.54	99.72	/	[20]
Barnettozyma californica K1	海洋表层沉积物	99.11	98.84	99.13	90.16	[21]
Glutamicibacter sp. WS1	活性污泥	100.00	98.10	99.87	/	[22]

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