Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (3): 118-134.doi: 10.13560/j.cnki.biotech.bull.1985.2023-0857

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Strategies for Increasing Heterologous Protein Expression in Pichia pastoris

RUZHA Yelizhati(), YANG Yu()   

  1. School of Life Science, Beijing Institute of Technology, Beijing 100081
  • Received:2023-09-04 Online:2024-03-26 Published:2024-04-08
  • Contact: YANG Yu E-mail:3120211414@bit.edu.cn;yooyoung@bit.edu.cn

Abstract:

The production of heterologous proteins via recombinant expression system is a focus of genetic engineering research. Because of its ease of genetic manipulation, high degree of heterologous protein secretion, and post-translational modification, Pichia pastoris, a methylotrophic yeast, has become an essential strain for protein production in industrial applications and is frequently employed in the manufacturing of enzymes. Although some heterologous proteins can be expressed in P. pastoris, the amount of expression is modest and has to be increased. Therefore, it is critical to investigate the principle and method of boosting the expression of heterologous proteins in P. Pastoris in order to lower industrial production costs and improve economic benefits. In this review, the optimizing strategies for improving heterologous protein expression in P. Pastoris in recent years are summarized from six perspectives: Gene level, transcription level, translation level, protein folding and secretion levels, cell resistance level, and fermentation technology, with the goal of demonstrating comprehensively how to improve the expression of heterologous protein in P. Pastoris expression system.

Key words: Pichia pastoris, recombinant expression, heterologous protein, optimization, enzyme