Analysis of Differential Metabolites and Bacterial Community Structure in the Soils of a Pineapple Orchard under Different Mulching Treatments

doi:10.13560/j.cnki.biotech.bull.1985.2023-1183

Abstract

Abstract:

【Objective】 The aim of this work is to investigate the effects of different mulching treatments on the soil microecological environment in pineapple orchard. 【Method】 Taking the un-mulched soil as the control（CK）, the differences in physicochemical properties, microbial abundance, bacterial community structure, and metabolites of the soils of mulching treatments with polyethylene mulch（PM）, non-woven fabric（NW）and biodegradable film（BF）were compared and measured, respectively. 【Result】 Compared with CK, PM significantly increased the activity of protease, whereas, decreased the activities of catalase and sucrase. NW significantly increased the content of available P, and decreased the activities of catalase and sucrase. Similarly, BF significantly increased the contents of total N, available P, and the activity of acid phosphatase, and decreased soil pH and the activities of catalase and sucrase. Compared with CK, PM significantly increased the community of actinomycetes. NW significantly increased the community of bacteria, while decreased the actinomycetes. BF significantly increased the communities of bacteria and fungi, while decreased the actinomycetes. The bacterial 16S high-throughput sequencing profiling suggested that the diversity of CK was higher than those of the three mulching treatments, and the bacteria abundance of BF was the highest among the three treatments and CK. Metabolites analysis indicated that there were 17 differential accumulated metabolites in the soils of three comparison groups with CK, mainly including carbohydrates, acids, nitrogen compounds and alcohols. And BF accumulated the highest sugar metabolites in the three mulching treatments. CCA analysis showed that soil pH affected the differentiation of bacterial community structure, and soil nutrients and enzyme activities were positively correlated with bacterial community structure. 【Conclusion】 Among the three mulching treatments, NW would benefit to maintain the relatively stable microecological environment of soil during the vegetative growth period of pineapple.

Key words: mulching-practices, pineapple, physiochemical properties of soil, soil metabolites, bacterial community structure

LIU Chuan-he, HE Han, SHAO Xue-hua, HE Xiu-gu. Analysis of Differential Metabolites and Bacterial Community Structure in the Soils of a Pineapple Orchard under Different Mulching Treatments[J]. Biotechnology Bulletin, 2024, 40(7): 247-258.

Figures/Tables 12

References 45

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处理 Treatment	pH	有机质 Organic matter/（g·kg^-1）	全氮 Total N/（g·kg^-1）	有效磷 Available P/（mg·kg^-1）	速效钾 Available K/（mg·kg^-1）
CK	3.99±0.06 a	36.83±1.62 a	1.53±0.03 b	57.00±5.49 b	24.33±2.40 b
PM	3.87±0.10 ab	35.87±1.01 a	1.50±0.06 b	68.50±7.05 ab	14.33±1.67 b
NW	4.09±0.12 a	37.57±1.15 a	1.57±0.03 b	105.27±19.06 a	16.67±0.67 b
BF	3.62±0.07 b	38.23±1.37 a	1.97±0.07 a	95.67±15.08 ab	69.33±22.41 a

处理 Treatment	pH	有机质 Organic matter/（g·kg^-1）	全氮 Total N/（g·kg^-1）	有效磷 Available P/（mg·kg^-1）	速效钾 Available K/（mg·kg^-1）
CK	3.99±0.06 a	36.83±1.62 a	1.53±0.03 b	57.00±5.49 b	24.33±2.40 b
PM	3.87±0.10 ab	35.87±1.01 a	1.50±0.06 b	68.50±7.05 ab	14.33±1.67 b
NW	4.09±0.12 a	37.57±1.15 a	1.57±0.03 b	105.27±19.06 a	16.67±0.67 b
BF	3.62±0.07 b	38.23±1.37 a	1.97±0.07 a	95.67±15.08 ab	69.33±22.41 a

处理 Treatment	脲酶 Urease/ （mg·（kg·h）^-1）	过氧化氢酶Catalase/（mL·（g·h）^-1）	酸性磷酸酶 Acid phosphatase/（µg·（g·h）^-1）	蔗糖酶 Sucrase/ （mL·（kg·h）^-1）	纤维素酶 Cellulase/ （mL·（kg·h）^-1）	蛋白酶 Protease/ （µg·（g·h）^-1）
CK	213.33±3.48 a	3.20±0.39 a	172.33±17.85 b	26.02±3.85 a	1.44±0.30 a	1.21±0.12 b
PM	185.33±2.40 a	0.77±0.14 c	166.00±7.51 b	3.34±0.23 b	1.82±0.30 a	4.56±0.43 a
NW	198.67±4.48 a	1.85±0.26 b	169.33±8.84 b	9.58±1.97 b	0.58±0.08 b	2.17±0.23 b
BF	227.67±34.27 a	1.83±0.30 b	240.33±5.33 a	5.10±1.17 b	1.33±0.19 ab	1.86±0.44 b

处理 Treatment	脲酶 Urease/ （mg·（kg·h）^-1）	过氧化氢酶Catalase/（mL·（g·h）^-1）	酸性磷酸酶 Acid phosphatase/（µg·（g·h）^-1）	蔗糖酶 Sucrase/ （mL·（kg·h）^-1）	纤维素酶 Cellulase/ （mL·（kg·h）^-1）	蛋白酶 Protease/ （µg·（g·h）^-1）
CK	213.33±3.48 a	3.20±0.39 a	172.33±17.85 b	26.02±3.85 a	1.44±0.30 a	1.21±0.12 b
PM	185.33±2.40 a	0.77±0.14 c	166.00±7.51 b	3.34±0.23 b	1.82±0.30 a	4.56±0.43 a
NW	198.67±4.48 a	1.85±0.26 b	169.33±8.84 b	9.58±1.97 b	0.58±0.08 b	2.17±0.23 b
BF	227.67±34.27 a	1.83±0.30 b	240.33±5.33 a	5.10±1.17 b	1.33±0.19 ab	1.86±0.44 b

处理Treatment	细菌Bacteria（×10⁶ CFU·g^-1）	真菌Fungi（× 10³ CFU·g^-1）	放线菌Actinomycetes（×10⁴ CFU·g^-1）
CK	2.45±0.52 b	8.07±1.62 b	8.2±1.02 a
PM	1.7±0.25 b	2.98±2.52 b	1.81±1.08 b
NW	4.23±0.79 a	5.66±0.65 b	2.51±1.92 b
BF	4.26±0.27 a	17.93±3.76 a	1.42±0.57 b