Research Progress in the Control of Multidrug Resistant Bacteria Based on in CRISPR-Cas System

doi:10.13560/j.cnki.biotech.bull.1985.2024-0872

Abstract

Abstract:

Antimicrobial resistance of animal-derived bacteria affects the safety of animal breeding and causes an important threat to human public health and food safety. The abuse of antibiotics intensifies the spread of drug-resistant bacteria, and the research and development of new antibacterial drugs is becoming increasingly difficult. Drug-resistant bacteria contamination of animal-derived bacteria has become a global public crisis. Once drug-resistant bacteria spread from animals to humans, it will greatly threaten human health, and we urgently need new methods and strategies to deal with bacterial resistance. CRISPR-Cas (clustered regularly interspaced short palindromic repeats CRISPR-associated) is the third generation of "genome point editing technology". This technology can target the cutting of exogenous DNA and protect the genetic stability of microbial genetic material. Compared with traditional multi-drug-resistant bacteria control strategies, CRISPR-Cas system has a unique DNA sequence targeting and sensitivity. The sensitivity and detection timeliness and other performance indicators can be improved through accurate, simple and efficient gene editing technology, combined with nucleic acid amplification technology, colorimetric technology. This paper introduces the origin, systematic classification, and mechanism of gene editing of CRISPR-Cas system. Based on this brief introduction, it focuses on the research progress and application of the system in the field of multi-drug-resistant bacteria prevention and control, and have case studies and current challenges in the elimination of drug-resistant pathogens, the elimination of drug-resistant genes, and the diagnosis and detection of pathogens. In general, sequence-specific antimicrobials based on CRISPR-Cas system may reduce bacterial multidrug resistance, and the combination of nucleic acid amplification technology and real-time monitoring equipment may improve the accuracy and efficiency of detection, providing a new idea for the prevention and control of drug resistance and monitoring of animal-derived bacteria.

Key words: CRISPR-Cas system, gene editing, multidrug resistant bacteria, antibiotic resistance genes, control technology

ZHOU Qian, TANG Meng-jun, ZHANG Xiao-yan, LU Jun-xian, TANG Xiu-jun, YANG Xing-xing, GAO Yu-shi. Research Progress in the Control of Multidrug Resistant Bacteria Based on in CRISPR-Cas System[J]. Biotechnology Bulletin, 2025, 41(5): 42-51.

Figures/Tables 4

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类型 Type		适配 Adaption	表达 Expression	干扰 Interference	辅助 Ancillary
Class 1	Ⅰ type	Cas1-Cas2	Cas6	Cas7-Cas5-Cas8-Cas3	Cas4
	Ⅲ type	Cas1-Cas2	Cas6
	Ⅳ type			Cas7-Cas5
Class 2	Ⅱ type	Cas1-Cas2	RNase Ⅲ	Cas9	Cas4
	V type	Cas1-Cas2		Cas12	Cas4
	Ⅵ type	Cas1-Cas2		Cas13

类型 Type		适配 Adaption	表达 Expression	干扰 Interference	辅助 Ancillary
Class 1	Ⅰ type	Cas1-Cas2	Cas6	Cas7-Cas5-Cas8-Cas3	Cas4
	Ⅲ type	Cas1-Cas2	Cas6
	Ⅳ type			Cas7-Cas5
Class 2	Ⅱ type	Cas1-Cas2	RNase Ⅲ	Cas9	Cas4
	V type	Cas1-Cas2		Cas12	Cas4
	Ⅵ type	Cas1-Cas2		Cas13

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