Advance in Plant Mitochondrial Genome Editing

doi:10.13560/j.cnki.biotech.bull.1985.2024-0638

Abstract

Abstract:

Mitochondria, the semi-autonomous organelles within eukaryotic cells, harbor specific genomes（mtDNA）and play pivotal roles in cellular life processes. mtDNA mutations in human are linked to a spectrum of genetic disorders, while in plants, recombination in mtDNA often leads to ORF genes associated with male sterility. The advent of gene editing technologies has revolutionized the study and treatment of mitochondrial diseases. With a plethora of mtDNA editing tools available, these technologies have also been instrumental in exploring functional genes and unknown sequences within plant mitochondrial genomes. Compared to nuclear genome editing, mtDNA editing still encounters certain challenges. This paper provides an overview of the development and current state of mtDNA editing technologies, examines their application in plant research, and contemplates future optimization strategies and the potential impact of these technologies on plant mitochondrial studies.

Key words: mitochondria, gene editing, ZFN, TALEN, CRISPR

ZHANG Shuo, KAN Jun-hu, ZHOU Jia-wei, WU Zhi-qiang. Advance in Plant Mitochondrial Genome Editing[J]. Biotechnology Bulletin, 2024, 40(10): 41-52.

Figures/Tables 3

References 88

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编辑工具 Editing tool	物种 Species	靶基因 Target gene	编辑类型 Editing type	表型 Phenotype	文献 Reference
mitoTALEN	水稻Oryza sativa	orf79	敲除	恢复花粉育性	[61]
mitoTALEN	油菜 Brassica napus	orf125	敲除	恢复花粉育性	[61]
mitoTALEN	拟南芥Arabidopsis thaliana	atp6-1, atp6-2	敲除	功能冗余；双突致死	[69]
mitoTALEN	水稻Oryza sativa	orf352	敲除	恢复花粉育性；不结实	[62]
mitoTALEN	水稻Oryza sativa	orf312	敲除	恢复花粉育性	[63]
mitoTALEN	番茄Solanum lycopersicum	orf137	敲除	恢复花粉育性	[65]
TALEN-GDM	烟草 Nicotiana tabacum	nad9	点突变	无明显表型	[71]
TALEN-GDM	烟草Nicotiana tabacum	nad9	敲除	生长迟缓；叶片和花发育缺陷；雄性不育	[72]
mitoTALEN	拟南芥Arabidopsis thaliana	nad7	敲除	严重的生长迟缓；致死	[70]
mitoTALEN	水稻Oryza sativa	WA352	敲除	恢复花粉育性	[64]
mitoTALEN	西兰花Brassica oleracea	orf138	敲除	恢复花粉育性	[66]
mitoTALEN	马铃薯Solanum tuberosum	orf125	敲除	N/A	[79]
mt-DdCBEs	油菜Brassica napus	atp6, rps14	C-to-T	N/A	[73]
mt-DdCBEs	莴苣Lactuca sativa	atp6	C-to-T	N/A	[73]
mitoTALECD	拟南芥Arabidopsis thaliana	atp1	C-to-T	恢复正常生长	[74]
mitoTALECD	马铃薯Solanum tuberosum	orf125	C-to-T	N/A	[79]
mTALED	水稻Oryza sativa	atp6-2	A-to-G	N/A	[77]
mitoCRISPR/Cas9	烟草Nicotiana tabacum	atp9	敲除	雄性不育	[78]

编辑工具 Editing tool	物种 Species	靶基因 Target gene	编辑类型 Editing type	表型 Phenotype	文献 Reference
mitoTALEN	水稻Oryza sativa	orf79	敲除	恢复花粉育性	[61]
mitoTALEN	油菜 Brassica napus	orf125	敲除	恢复花粉育性	[61]
mitoTALEN	拟南芥Arabidopsis thaliana	atp6-1, atp6-2	敲除	功能冗余；双突致死	[69]
mitoTALEN	水稻Oryza sativa	orf352	敲除	恢复花粉育性；不结实	[62]
mitoTALEN	水稻Oryza sativa	orf312	敲除	恢复花粉育性	[63]
mitoTALEN	番茄Solanum lycopersicum	orf137	敲除	恢复花粉育性	[65]
TALEN-GDM	烟草 Nicotiana tabacum	nad9	点突变	无明显表型	[71]
TALEN-GDM	烟草Nicotiana tabacum	nad9	敲除	生长迟缓；叶片和花发育缺陷；雄性不育	[72]
mitoTALEN	拟南芥Arabidopsis thaliana	nad7	敲除	严重的生长迟缓；致死	[70]
mitoTALEN	水稻Oryza sativa	WA352	敲除	恢复花粉育性	[64]
mitoTALEN	西兰花Brassica oleracea	orf138	敲除	恢复花粉育性	[66]
mitoTALEN	马铃薯Solanum tuberosum	orf125	敲除	N/A	[79]
mt-DdCBEs	油菜Brassica napus	atp6, rps14	C-to-T	N/A	[73]
mt-DdCBEs	莴苣Lactuca sativa	atp6	C-to-T	N/A	[73]
mitoTALECD	拟南芥Arabidopsis thaliana	atp1	C-to-T	恢复正常生长	[74]
mitoTALECD	马铃薯Solanum tuberosum	orf125	C-to-T	N/A	[79]
mTALED	水稻Oryza sativa	atp6-2	A-to-G	N/A	[77]
mitoCRISPR/Cas9	烟草Nicotiana tabacum	atp9	敲除	雄性不育	[78]