Fig. 1 Bioinformatics characteristics of RcXYNC geneA: Electrophoresis map of gene coding sequence cloning. B: Coding sequence of RcXYNC. C: Predicted conservative structure domain of RcXYNC. D: Multiple sequences alignment of RcXYNCwith other species. E: Phylogenetic analysis of RcXYNC (The location of RcXYNC marked with a '▲')

Fig. 2 Predicted secondary structure, tertiary structure, signal peptides and transmembrane domain of RcXYNCproteinA: Predicted secondary structure of RcXYNCprotein. B: Predicted tertiary structure of RcXYNCprotein. C: Predicted signal peptides of RcXYNC. D: Predicted transmembrane domain of RcXYNC

Fig. 3 Subcellular localization of RcXYNC proteinGFP: Green fluorescent protein. Bright: Bright field. Merge: Integration of all fields

Fig. 4 Relative expression of RcXYNC in rose inoculated by H2O or M. rosaens: No significance; *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.000 1. The same below

Fig. 5 Analysis of RcXYNC promoter sequence and cis-acting elementsA: Electrophoresis map of gene promoter cloning. B: RcXYNC promoter sequence. C: Diagram of the cis-acting elements of the RcXYNC promoter

Fig. 6 Transient overexpression of RcXYNC enhanced the sensitiveness of rose to M. rosaeA: The black spot phenotype of R12-26 infected with M. rosae under RcXYNC overexpression on 8 d. B: Relative expression of RcXYNC of R12-26 infected with M. rosae under RcXYNC overexpression on 5 d. C: The black spot area statistic of R12-26 infected with M. rosae under RcXYNC overexpression on 8 d. D: The black spot phenotype of R13-54 infected with M. rosae under RcXYNC overexpression on 8 d. E: Relative expression of RcXYNC of R13-54 infected with M. rosae under RcXYNC overexpression on 5 d. F: The black spot area statistic of R13-54 infected with M. rosae under RcXYNC overexpression on 8 d. G: The H2O2 accumulation area statistic of R12-26 and R13-54 infected with M. rosae under RcXYNC overexpression on 2 d. H: The H2O2 accumulation phenotype of R12-26 and R13-54 infected with M. rosae under RcXYNC overexpression on 2 d. I: Relative expression of defense genes in R12-26 infected with M. rosae under RcXYNC overexpression on 2 d. J: Relative expression of defense genes in R13-54 infected with M. rosae under RcXYNC overexpression on 2 d (WT: rose leaves inoculated with M. rosae; PVX or PVX::RcXYNC; rose leaves infiltrated with Agrobacterium suspensions carrying PVX empty vector or PVX::RcXYNC recombinant vector and subsequently inoculated with M. rosae)

Fig. 7 Silencing of RcXYNC enhanced the resistance of rose to M. rosaeA: The black spot phenotype of R12-26 infected with M. rosae under RcXYNC silencing on 8 d. B: Relative expression of RcXYNC of R12-26 infected with M. rosae under RcXYNC silencing on 5 d. C: The black spot area statistic of R12-26 infected with M. rosae under RcXYNC silencing on 8 d. D: The black spot phenotype of R13-54 infected with M. rosae under RcXYNC silencing on 8 d. E: Relative expression of RcXYNC of R13-54 infected with M. rosae under RcXYNC silencing on 5 d. F: The black spot area statistic of R13-54 infected with M. rosae under RcXYNC silencing on 8 d. G: The H2O2 accumulation area statistic of R12-26 and R13-54 infected with M. rosae under RcXYNC silencing on 2 d. H: The H2O2 accumulation phenotype of R12-26 and R13-54 infected with M. rosae under RcXYNC silencing on 2 d. I: Relative expression of defense genes in R12-26 infected with M. rosae under RcXYNC silencing on 2 d. J: Relative expression of defense genes in R13-54 infected with M. rosae under RcXYNC silencing on 2 d (WT: rose leaves inoculated with M. rosae; TRV or TRV::RcXYNC; rose leaves infiltrated with Agrobacterium suspensions carrying TRV empty vector or TRV::RcXYNC recombinant vector and subsequently inoculated with M. rosae)

Fig. 8 Verifying the interaction between RcXYNC and candidate proteins via yeast two-hybridA: Self-activation identification of bait vector. B: Gene cloning of candidate proteins. (Lane M: DL2000 marker; other lanes: CDS cloning of candidate proteins) C: One-to-one validation of RcXYNC interaction with candidate target proteins in yeast (AD: pGADT7; BD: pGBKT7; AD-T+BD-Lam, AD+BD and AD+BD-RcXYNC: negative control; AD-T+BD-53: positive control; 10-1, 10-2, 10-3, 10-4: the dilution gradients of bacterial solution)