Abstract: The aim was to isolate Pyrroloquinoline quinine producing strains from endophytic bacteria of Polygonum cuspidatumand strains of myxobacteria. Three kinds of media with methanol as single carbon source were used for microbial fermentation of the strains.Spectroscopy and HPLC analysis of the fermentation product were performed. Through preliminary screening and complex screening we got 134strains of methanol-utilizing and 4 strains of Pyrroloquinoline quinine producing. The Pyrroloquinoline quinine producing strain 083114 wasdetermined to be 64.34 mg/L. The 16S rRNA sequence analysis showed that the phylogenetic relationship between strain 083114 and Bacillusacidiceler is the most similar. Therefore, it proved that Pyrroloquinoline quinine producing bacteria were in endophytic bacteria of Polygonumcuspidatum and myxobacteria.

Key words: Methanol-utilizing bacteria , Pyrroloquinoline quinine , Endophytic bacteria , Myxobacteria , Spectroscopy