Abstract: In this paper, four millet varieties “JI GU11”, “YU GU2”, “TIE GU5” and “SHI LI XIANG” seeds were used to induce calli, and the factors which influence callus induce rate and seedling regenerate rate were stuied. Variable frequencies of callus initiation were observed among the varieties. The vareity “JIGU11” exhibited high callus induction efficiency and selected for further study. Four media, three carbon sources at different concentrations, different concentrations of tryptophan, casein hydrolysate, gelrite and sorbitol were tested. The concentrations and combination of different hormones were also examined. The results suggested that the optimal callus induction medium was LS supplemented with 2 mg/L 2, 4-D combination with 1 mg/L ZT and 50 g/L maltose, 1 g/L casein hydrolysate, 50 mg/L tryptophan, 4% sorbitol and 0.5% gelrite. While maximum regeneration response was detected on MSB5 medium fortified with 0.5 mg/L NAA+2.0 mg/L 6-BA, 30 g/L maltose plus 4% sorbitol.

Key words: Millet Callus, Induction, Regeneration