Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (3): 83-89.

• Research report • Previous Articles     Next Articles

Cloning and Bioinformation Analysiss of StSnRK2.1 Gene in Solanum tuberosum

Liu Siyan, Mao Juan, Fan Aqi, Zhang Junlian, Wang Di, Bai Jiangping   

  1. Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement,Gansu Provincial Key Laboratory of Aridland Crop Science,College of Agronomy,Gansu Agricultural University,Lanzhou 730070)
  • Received:2012-09-18 Revised:2013-03-21 Online:2013-03-20 Published:2013-03-21

Abstract: SnRK2s plays an important role in the regulation when plants suffer from adervise environments. The full-length cDNA sequence of SnRK2.1 in Solanum tuberosum was successfully cloned by RT-PCR with the tube plant. The gene was named StSnRK2.1 and has been submitted to Genbank with the accession number JX280911.By bioinformation analysis, the results showed that the StSnRK2.1 has a complete ORF sequence is 1 008 bp, encoding 335 predicted amino acids. The protein molecular weight is 37.77 kD and theoretical pI is 5.37. In protein’s secondary structure, Alpha helix is 42.39%, Extended strand 16.42%, Bela turn is 7.46%, Random coil is 33.73%. Also, the protein is drophilicity, haven’t transmembrane. It is probably exit in the cytoplasm and microbody by subcellular localization prediction of protein. There are seven serine sites, two threonine sites, three tyrosine sites, so it is putative that the StSnRK2.1 is related to plant’s tolerance in abiotic stress.

Key words: Solanum tuberosum, Anti-drought, Cloning, Bioinformation analysis