Ammonia Eexcretion Related Genes Expression of Common Carp Under the Stress of Carbonate Alkalinity

Abstract

Abstract: Common carp（Cyprinus carpio）is one of the most important aquaculture species in China and with certain alkalisaline tolerance. In order to understand the common carp genes expression in different alkali-saline water, we studied the candidate ammonia excretion related genes ：Rh（Rhesus blood group-associated glycoprotein）, AQP（Aquaporin）, PRL（Prolactin）, CA（Carbonic Anhydrase）, NKA（Na⁺/K⁺ transporting ATPase）, NHE（Sodium Hydrogen Exchanger）expression at a rising carbonate alkalinity gradient in a certain pH value by quantitative PCR method. Results showed that with the rise of carbonate alkalinity value, the overall expression of these genes in gill, kidney and intestine are up-regulated compared with freshwater controls. And AQP3A, PRLRA, NHE7 are mainly expressed in gill ；PRL are mainly expressed in kidney ；RHAG, RHBG2, RHCG1 are mainly expressed in intestine under the stress of carbonate alkalinity. The changes of these genes expression in different level of carbonate alkalinity can provide insights into the mechanism of osmotic regulation for fish.

Key words: Ammonia excretion, Gene expression, Carbonate alkalinity, Quantitative PCR

Zhao Lan, Xu Peng, Sun Xiaowen. Ammonia Eexcretion Related Genes Expression of Common Carp Under the Stress of Carbonate Alkalinity[J]. Biotechnology Bulletin, 2013, 0(4): 185-193.

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