Cloning and Sequence Analysis of PsaH Gene from Sasussured involucrata Kar. et Kir

Abstract

Abstract: Ps I H is an intrinsic membrane protein of 10 kD that is a subunit of photosystem I(Ps I), Ps I H is one of the three PSI subunits found only in eukaryotes, plays an important role in plant photosynthesis. A cDNA clone encoding a PS I H subunit of photosystem I was cloned from Sasussured involucrata Kar. et Kir leaf cDNA library. Sequence analysis indicated that the psaH cDNA contained an open reading frame of 432 nucleotides encoding a 143 amino acid which consisted of a 47 amino acid transit peptide and a 96 amino acid mature PS I H peptide. Forecasted the protein relative molecular weight is 15.04 kD, Theoretical pI was 9.81, contains a span membrane structure. Sequence alignment indicated that the deduced amino acid sequence of Sasussured involucrata Kar. et Kir showed high identity of 83.3% to those of PsaH from Ricinus communis and Populus trichocarpa. Phylogenetic analysis further showed that the SikPsaH gene has the nearest relationship with Lycoris radiate.

Key words: Sasussured involucrata, Kar. et, Kir PsaH gene, Cloning, Sequence analysis

Zhang Linhua, Qiu Honglin, Liu Chao, Wang Aiying, Deng Fujun, Zhu Jianbo. Cloning and Sequence Analysis of PsaH Gene from Sasussured involucrata Kar. et Kir[J]. Biotechnology Bulletin, 2013, 0(6): 75-79.

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