Glutathione Biosynthesis by Engineered Saccharomyces cerevisiae

Abstract

Abstract: The GSH1 and GSH2 genes were amplified from the extracted total DNA of Saccharomyces cerevisiae S288c by PCR and expressed under the control of alcohol dehydrogenase(ADH1)promoter. With Kan^r or Hyg^r as the selective markers, two expression vectors YEplace181AK-GSH1 and YEplace181AH-GSH2 were constructed. The recombinant plasmids were transformed into an industry strain Saccharomyces cerevisiae TS013 respectively and simultaneously by electroporation, and three recombinant strains S.TS013/GSH1, S.TS013/GSH2 and S.TS013/GSH1+GSH2 were generated by screening on YPD plates supplemented with G418 or（and）Hygromycin. The transformants were cultured in fermentation media, afterwards intracellular GSH content was detected by the method of ALLOXAN. Compared with the content of control strain, the glutathione content of recombinant strains increased by 44.11%, 29.79% and 56.47%, respectively.

Key words: γ-Glutamylcysteine synthetase, GSH synthetase, Glutathione, Saccharomyces cerevisiae

Chen Jiali, Wu Liang, Wang Juan, Duan Xuehui. Glutathione Biosynthesis by Engineered Saccharomyces cerevisiae[J]. Biotechnology Bulletin, 2013, 0(8): 160-165.

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