生物技术通报 ›› 2018, Vol. 34 ›› Issue (1): 110-118.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0621

• 研究报告 • 上一篇    下一篇

小桐子MYB基因家族的鉴定及JcMYB308基因的克隆与低温表达分析

辛胡1,2, 颜岳辉1,2, 丁雪梅1,2, 刘潮2, 高永2, 代冬琴2, 唐利洲2, 王海波2   

  1. 1. 西南林业大学林学院,昆明 650224;
    2. 曲靖师范学院 云南高原生物资源保护与利用研究中心 云南省高校云贵高原动植物遗传多样性及生态适应性重点实验室,曲靖 655011
  • 收稿日期:2017-07-26 出版日期:2018-01-26 发布日期:2018-01-22
  • 作者简介:辛胡,女,硕士研究生,研究方向:植物逆境分子生物学;E-mail:2459343427@qq.com
  • 基金资助:
    国家自然科学基金项目(31460179)

Genome-wide Identification of MYB Gene Family and Cloning & Expression Analysis of JcMYB308 Gene from Jatropha curcas

XIN Hu1,2, YAN Yue-hui1,2, DING Xue-mei1,2, LIU Chao2, GAO Yong2, DAI Dong-qin2, TANG Li-zhou2, WANG Hai-bo2   

  1. 1. College of Forestry,Southwest Forestry University,Kunming 650224;
    2. Center for Yunnan Plateau Biological Resources Protection and Utilization,Qujing Normal University,Key Laboratory of Yunnan Province Universities of the Diversity and Ecological Adaptive Evolution for Animals and Plants on Yungui Plateau,Qujing Normal University,Qujing 655011
  • Received:2017-07-26 Published:2018-01-26 Online:2018-01-22

摘要: MYB转录因子家族参与植物生长发育及对环境胁迫的应答等过程。该实验基于小桐子基因组数据库,对MYB基因家族进行鉴定,克隆了小桐子JcMYB308基因,并对其功能结构域、系统进化、基因结构及低温表达特性进行了分析。结果表明,小桐子全基因组共鉴定到213个MYB基因家族成员,聚类为6个亚家族。克隆的JcMYB308基因片段长度为713 bp,基因结构具有较高的保守性,均含有两个外显子,进化树显示其与同属大戟科的蓖麻亲缘关系最近,序列一致性为62.7%。qRT-PCR表达分析表明,小桐子JcMYB308基因的表达存在组织表达特异性,在根中表达量较高,而在叶片中表达量相对较低,在根与茎中低温胁迫24 h时达到最大表达量。

关键词: 小桐子, MYB, 基因家族, MYB308, 基因克隆, 表达分析

Abstract: MYB transcription factor family involves in the processes of the plant growth and development and the response to environmental stresses. Based on the database of Jatropha curcas genome,MYB308 gene family was identified,then JcMYB308 of J. curcas was cloned,and the functional domains,phylogenetic relationship,gene structure,and chilling expression characteristics were analyzed. The results demonstrated that the whole genome was identified to have 213 members of gene family,and they were clustered as 6 subfamilies. The cloned JcMYB308 gene fragment length was 713 bp,and the gene structure was highly conserved,with two exons. In addition,the phylogenetic tree showed that its amino acid sequence shared 62.7% identity with Ricinus communis that belonged to the same family of Euphorbiaceae. qRT-PCR expression analysis revealed that JcMYB308 expressed in different organs,abundantly in root,but scarcely in leaves,and at the highest level in stem and root at 24 h after cold-induction.

Key words: Jatropha curcas, MYB, gene family, MYB308, gene cloning, expression analysis