基于代谢组学研究谷子SiYABBYs参与花发育过程中鼠李糖苷的生物合成

doi:10.13560/j.cnki.biotech.bull.1985.2022-1463

生物技术通报 ›› 2023, Vol. 39 ›› Issue (6): 189-198.doi: 10.13560/j.cnki.biotech.bull.1985.2022-1463

基于代谢组学研究谷子SiYABBYs参与花发育过程中鼠李糖苷的生物合成

韩华蕊¹(), 杨宇琭¹, 门艺涵¹, 韩尚玲¹, 韩渊怀¹^,²^,³, 霍轶琼¹^,²(), 侯思宇¹^,²^,³()

1.山西农业大学农学院，太谷 030801
2.山西农业大学农业生物工程研究所，太谷 030801
3.杂粮种质创新与分子育种山西省重点实验室，太谷 030801

收稿日期:2022-11-29 出版日期:2023-06-26 发布日期:2023-07-07
通讯作者: 侯思宇，男，副教授，研究方向：种质创新与遗传工程；E-mail: bragren123@163.com；
霍轶琼，女，讲师，研究方向：谷子米色基因功能；E-mail: yiqionghuo2013@126.com
作者简介:韩华蕊，女，硕士研究生，研究方向：种质创新与遗传工程；E-mail: 2594488988@qq.com
基金资助:
谷子种业创新良种联合攻关项目(2022XCZX04-10);山西省高等学校科技创新项目(2021L103);山西农业大学科技创新基金项目-博士科研启动(2020BQ12);杂粮种质创新与分子育种山西省重点实验室

SiYABBYs Involved in Rhamnoside Biosynthesis During the Flower Development of Setaria italica, Based on Metabolomics

HAN Hua-rui¹(), YANG Yu-lu¹, MEN Yi-han¹, HAN Shang-ling¹, HAN Yuan-huai¹^,²^,³, HUO Yi-qiong¹^,²(), HOU Si-yu¹^,²^,³()

1. College of Agriculture, Shanxi Agricultural University, Taigu 030801
2. Institute of Agricultural Bioengineer, Shanxi Agricultural University, Taigu 030801
3. Shanxi Key Laboratory of Germplasm Innovation and Molecular Breeding in Minor Crops, Taigu 030801

Received:2022-11-29 Published:2023-06-26 Online:2023-07-07

摘要/Abstract

摘要：

花器官发育是生殖发育的前提和基础，对小穗最终的形成具有决定性作用，YABBY转录因子在花器官的发育中具有重要作用，探究谷子YABBY家族成员的序列特征及表达模式，揭示其与谷子穗发育的关系，为阐明谷子YABBY基因家族的功能以及提高谷子产量奠定理论基础。通过对谷子YABBY基因家族进行全基因组鉴定，分析SiYABBYs的理化性质、组织表达模式，并与拟南芥和水稻YABBY蛋白序列比对进行功能预测。结果表明，共鉴定8个YABBY家族成员，编码150-303个氨基酸，在抽穗期和授粉期的穗中特异表达，可能参与谷子营养组织的发育和花器官的形成。对谷子抽穗期和授粉期的代谢组数据与SiYABBYs的表达水平进行相关性分析发现，SiYABBY1/SiYABBY5/SiYABBY7/SiYABBY8与鼠李糖苷代谢通路的酶基因紧密关联，表明促进授粉期穗中鼠李糖苷的积累，可能与谷子的成育率及结实率相关。

关键词: 谷子, YABBY基因家族, 代谢组学

Abstract:

Flower organ is the premise and basis of reproductive development, and also plays a decisive role at the final stage of spikelet formation. YABBY transcription factors play an important role in the development of flower organs. In order to clarify the function of YABBY gene family and improve the yield of foxtail millet (Setaria italica), we studied the sequence characteristics and expression patterns of YABBY gene family members in the foxtail millet. We analyzed genome-wide identification of YABBY gene family in the foxtail millet, the physical and chemical properties, and tissue expression patterns of SiYABBYs gene. Also, we aligned YABBY sequences of the foxtail millet, Arabidopsis and rice（Oryza sativa）for functional prediction. The results showed that 8 YABBY family members in the foxtail millet, encoding 150-303 amino acids. And we found these SiYABBYs genes were specifically expressed in panicles at heading and pollination stages, which may be involved in the development of vegetative tissues and the formation of flower organs in the foxtail millet. The expressions of SiYABBYs were correlated with the metabolome data at heading and pollination stages of the foxtail millet. The results showed that SiYABBY1/SiYABBY5/SiYABBY7/SiYABBY8 were closely related to rhamnoside metabolic pathway. Also, the results indicated that rhamnoside accumulation in the panicles during the pollination might be related to the fertility and seed setting rate of the foxtail millet.

Key words: foxtail millet, YABBY family, metabolomics

韩华蕊, 杨宇琭, 门艺涵, 韩尚玲, 韩渊怀, 霍轶琼, 侯思宇. 基于代谢组学研究谷子SiYABBYs参与花发育过程中鼠李糖苷的生物合成[J]. 生物技术通报, 2023, 39(6): 189-198.

HAN Hua-rui, YANG Yu-lu, MEN Yi-han, HAN Shang-ling, HAN Yuan-huai, HUO Yi-qiong, HOU Si-yu. SiYABBYs Involved in Rhamnoside Biosynthesis During the Flower Development of Setaria italica, Based on Metabolomics[J]. Biotechnology Bulletin, 2023, 39(6): 189-198.

图/表 5

参考文献 36

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亚族 Subfamily	在拟南芥中的功能 Function in Arabidopsis thaliana	在水稻中的功能 Function in rice	谷子中功能预测 Function prediction in foxtail millet
YAB3	AtFIL：调控花序和花分生组织的形成和发育 ATFIL: Regulates the formation and development of inflorescence and floral meristems	OsYABBY3：侧生器官的形成和分生组织的分化 OsYABBY3: Formation of lateral organs and differentiation of meristems	SiYABBY1/SiYABBY5/SiYABBY7：参与侧生器官的发育以及花器官和小穗的形成和发育 SiYABBY1/SiYABBY5/SiYABBY7: Participate in lateral organ development and the formation and development of flower organs and spikelet
	AtYAB3：参与营养组织的发育 AtYAB3: Participate in the development of nutritive tissues	OsYABBY4：调控维管组织的发育和叶片的极性 OsYABBY4: Regulates vascular tissue development and leaf polarity
		OsYABBY5：小穗发育 OsYABBY5: Spikelets development
INO	AtINO：调节外胚珠珠被的发育 AtINO: To regulate the development of the outer ovules	OsYABBY7	SiYABBY3：调节外胚珠和花器官的发育 SiYABBY3: Adjust the ovule and floral organ development
CRC	AtCRC：心皮发育的关键调节因子 AtCRC: Carpel development a key regulating factor	OsDL：调控心皮的形成 OsDL: Regulate the formation of carpels	SiYABBY8（SiDL）：导致开花延迟、叶片卷曲和种子变小以及参与心皮的形成 SiYABBY8（SiDL）: Cause delayed flowering, smaller hairy leaves and seeds, and participate in the formation of carpel
YAB2	AtYAB2：参与营养组织的发育 AtYAB2: Participate in the development of nutritive tissues	OsYABBY1：雄蕊和心皮的分生组织发育 OsYABBY1: Meristematic development of stamens and carpels	SiYABBY2/SiYABBY4/SiYABBY6：参与营养组织的发育和花器官的形成 SiYABBY2/SiYABBY4/SiYABBY6: Involved in the development of vegetative tissues and the formation of floral organs
		OsYABBY2
		OsYABBY6：调控水稻的株高和产量 OsYABBY6: Regulate the plant height and yield of rice

亚族 Subfamily	在拟南芥中的功能 Function in Arabidopsis thaliana	在水稻中的功能 Function in rice	谷子中功能预测 Function prediction in foxtail millet
YAB3	AtFIL：调控花序和花分生组织的形成和发育 ATFIL: Regulates the formation and development of inflorescence and floral meristems	OsYABBY3：侧生器官的形成和分生组织的分化 OsYABBY3: Formation of lateral organs and differentiation of meristems	SiYABBY1/SiYABBY5/SiYABBY7：参与侧生器官的发育以及花器官和小穗的形成和发育 SiYABBY1/SiYABBY5/SiYABBY7: Participate in lateral organ development and the formation and development of flower organs and spikelet
	AtYAB3：参与营养组织的发育 AtYAB3: Participate in the development of nutritive tissues	OsYABBY4：调控维管组织的发育和叶片的极性 OsYABBY4: Regulates vascular tissue development and leaf polarity
		OsYABBY5：小穗发育 OsYABBY5: Spikelets development
INO	AtINO：调节外胚珠珠被的发育 AtINO: To regulate the development of the outer ovules	OsYABBY7	SiYABBY3：调节外胚珠和花器官的发育 SiYABBY3: Adjust the ovule and floral organ development
CRC	AtCRC：心皮发育的关键调节因子 AtCRC: Carpel development a key regulating factor	OsDL：调控心皮的形成 OsDL: Regulate the formation of carpels	SiYABBY8（SiDL）：导致开花延迟、叶片卷曲和种子变小以及参与心皮的形成 SiYABBY8（SiDL）: Cause delayed flowering, smaller hairy leaves and seeds, and participate in the formation of carpel
YAB2	AtYAB2：参与营养组织的发育 AtYAB2: Participate in the development of nutritive tissues	OsYABBY1：雄蕊和心皮的分生组织发育 OsYABBY1: Meristematic development of stamens and carpels	SiYABBY2/SiYABBY4/SiYABBY6：参与营养组织的发育和花器官的形成 SiYABBY2/SiYABBY4/SiYABBY6: Involved in the development of vegetative tissues and the formation of floral organs
		OsYABBY2
		OsYABBY6：调控水稻的株高和产量 OsYABBY6: Regulate the plant height and yield of rice

转录因子 Transcription factor	结合基序 Motif ID	靶基因 Target gene	模板链 Strand	起始位置 Start	终止为止 End	P值 P-value	q值 q-value	匹配序列 Matched sequence
SiYABBY8	UN0396.1	Si1g24720	-	1 734	1 741	2.65e-05	1	TAATCATA
		Si1g07250	+	698	705	5.3e-05	1	TTATCATA
		Si1g24750	+	1 165	1 172	7.95e-05	1	TAATCATA
		Si7g05130	+	1 478	1 485	7.95e-05	1	TAATCATT
SiYABBY1 SiYABBY5 SiYABBY7	UN0415.1	Si8g14530	-	121	128	3.23e-05	0.529	AATAATAA
		Si9g17990	-	1 088	1 095	3.23e-05	0.529	AATAATAA
		Si7g30480	+	1 319	1 326	3.23e-05	0.529	AATAATAA
		Si9g30300	-	1 314	1 321	3.23e-05	0.529	AATAATAA
		Si7g01180	-	1 785	1 792	3.23e-05	0.529	AATAATAA
		Si7g01180	-	389	396	6.46e-05	0.529	AATAATTA
		Si6g16950	-	861	868	6.46e-05	0.529	AATAATTA
		Si6g16950	+	896	903	6.46e-05	0.529	AATAATTA
		Si7g30480	-	1 593	1 600	6.46e-05	0.529	AATAATTA
		Si9g04210	+	1 645	1 652	6.46e-05	0.529	AATAATTA
		Si9g04210	-	1 647	1 654	6.46e-05	0.529	AATAATTA
		Si7g01180	-	1 793	1 800	6.46e-05	0.529	AATAATTA
		Si7g05130	-	164	171	9.69e-05	0.56	AATATTAA
		Si9g52070	+	249	256	9.69e-05	0.56	AATATTAA
		Si7g01180	-	431	438	9.69e-05	0.56	AATATTAA
		Si9g30300	+	849	856	9.69e-05	0.56	AATATTAA
		Si7g30480	-	1 452	1 459	9.69e-05	0.56	AATATTAA
		Si5g36410	+	1 437	1 444	9.69e-05	0.466	AATATTAA

转录因子 Transcription factor	结合基序 Motif ID	靶基因 Target gene	模板链 Strand	起始位置 Start	终止为止 End	P值 P-value	q值 q-value	匹配序列 Matched sequence
SiYABBY8	UN0396.1	Si1g24720	-	1 734	1 741	2.65e-05	1	TAATCATA
		Si1g07250	+	698	705	5.3e-05	1	TTATCATA
		Si1g24750	+	1 165	1 172	7.95e-05	1	TAATCATA
		Si7g05130	+	1 478	1 485	7.95e-05	1	TAATCATT
SiYABBY1 SiYABBY5 SiYABBY7	UN0415.1	Si8g14530	-	121	128	3.23e-05	0.529	AATAATAA
		Si9g17990	-	1 088	1 095	3.23e-05	0.529	AATAATAA
		Si7g30480	+	1 319	1 326	3.23e-05	0.529	AATAATAA
		Si9g30300	-	1 314	1 321	3.23e-05	0.529	AATAATAA
		Si7g01180	-	1 785	1 792	3.23e-05	0.529	AATAATAA
		Si7g01180	-	389	396	6.46e-05	0.529	AATAATTA
		Si6g16950	-	861	868	6.46e-05	0.529	AATAATTA
		Si6g16950	+	896	903	6.46e-05	0.529	AATAATTA
		Si7g30480	-	1 593	1 600	6.46e-05	0.529	AATAATTA
		Si9g04210	+	1 645	1 652	6.46e-05	0.529	AATAATTA
		Si9g04210	-	1 647	1 654	6.46e-05	0.529	AATAATTA
		Si7g01180	-	1 793	1 800	6.46e-05	0.529	AATAATTA
		Si7g05130	-	164	171	9.69e-05	0.56	AATATTAA
		Si9g52070	+	249	256	9.69e-05	0.56	AATATTAA
		Si7g01180	-	431	438	9.69e-05	0.56	AATATTAA
		Si9g30300	+	849	856	9.69e-05	0.56	AATATTAA
		Si7g30480	-	1 452	1 459	9.69e-05	0.56	AATATTAA
		Si5g36410	+	1 437	1 444	9.69e-05	0.466	AATATTAA

基于代谢组学研究谷子SiYABBYs参与花发育过程中鼠李糖苷的生物合成

SiYABBYs Involved in Rhamnoside Biosynthesis During the Flower Development of Setaria italica, Based on Metabolomics

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