Genome-wide Bioinformatics Identification of PPR Gene Family and Expression Profiles Analysis in Wheat

doi:10.13560/j.cnki.biotech.bull.1985.2019-0165

Abstract

Abstract: The aim is to screen the PPR（Pentatricopeptide repeats）gene with fertility restoration function for the hybrids of the two-line hybrid wheat,and to further study the function and regulation mechanism of PPR genes. After bioinformatics analysis of wheat genome-wide data,1 351 PPR members were obtained. Then,the chromosome location and distribution of PPR gene family in wheat were analyzed,and the phylogenetic tree was constructed. Total 23 PPR genes related to fertility restoration were obtained by homology align and analysis of them with reported fertility restorer genes in rice. Real-time fluorescence quantitative PCR was performed to screen and identify the expression pattern of 23 fertility restoration genes on young spikes of 2 different restorer lines of high and low,one sterile line,and their F₁ generation in two-line hybrid wheat at meiotic stage. Based on phenotypic analysis of important agronomic traits such as seed setting rate,it was found that the 4 PPR genes showed obvious over-dominance patterns in the high-restorer hybrid combinations,and correspondingly these 4 genes basically presented low-parent dominance expression patterns in low-restorer line hybrid combinations. It is preliminarily confirmed that these 4 PPR genes may be involved in the regulation of fertility restoration of the two-line hybrid wheat.

Key words: wheat, PPR gene family, homology align, fertility restoration

GUO Cai-juan, GONG Jie, LIU Yong-jie, ZHAO Chang-ping, GAO Shi-qing, WU Hua-wei. Genome-wide Bioinformatics Identification of PPR Gene Family and Expression Profiles Analysis in Wheat[J]. Biotechnology Bulletin, 2019, 35(8): 1-8.

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