生物技术通报 ›› 2015, Vol. 31 ›› Issue (12): 110-114.doi: 10.13560/j.cnki.biotech.bull.1985.2015.12.016

• 研究报告 • 上一篇    下一篇

铅铬胁迫对小麦种子萌发及幼苗脯氨酸含量的影响

杨文玲,岳丹丹,李冠杰,刘莹莹,宁萌,刘莉,巩涛,王继雯,陈国参   

  1. 河南省科学院生物研究所有限责任公司,郑州 450008
  • 收稿日期:2015-03-23 出版日期:2015-12-19 发布日期:2015-12-19
  • 作者简介:杨文玲,女,博士,助理研究员,研究方向:环境污染修复;E-mail:wlyang0130@163.com
  • 基金资助:
    河南省科技开放合作项目(132106000056)

The Effects of Lead and Chromium Stresses on Seed Germination and Proline Content in Wheat Seedlings

Yang Wenling, Yue Dandan, Li Guanjie, Liu Yingying, Ning Meng, Liu Li, Gong Tao, Wang Jiwen, Chen Guocan   

  1. Institute of Biology,Limited Liability Company,Henan Academy of Sciences,Zhengzhou 450008
  • Received:2015-03-23 Published:2015-12-19 Online:2015-12-19

摘要: 旨在探讨铅铬胁迫对小麦的毒害效应以及对幼苗脯氨酸的影响。用不同浓度的铅铬溶液处理小麦,研究铅铬单一及复合胁迫对小麦种子萌发及幼苗脯氨酸含量的影响。结果表明,铅铬单一及复合胁迫均使得小麦种子萌发率降低,幼苗株高、根长降低,鲜物质量和干物质量减少。铅铬处理1 d后,各处理组小麦体内的脯氨酸含量较对照都有升高,尤其是复合胁迫下 100 mg/L Pb+100 mg/L Cr处理组,其小麦脯氨酸含量较对照升高了32.81%。铅铬处理3、5和7 d后,100 mg/L Cr、50 mg/L Cr、200 mg/L Pb+100 mg/L Cr、100 mg/L Pb+100 mg/L Cr这4个处理组的小麦脯氨酸含量较对照均显著升高,其它各处理组与对照相比差异不显著。铅铬胁迫抑制了小麦种子的萌发和幼苗生长,使得小麦体内脯氨酸含量升高,因此建议脯氨酸含量的变化可以作为监测植物铅铬胁迫的指标之一。

关键词: 铅, 铬, 种子萌发, 脯氨酸, 毒害

Abstract: The aim was to explore the toxic effects of lead and chromium stresses on wheat and the influence of them on wheat proline. Treating wheat with different concentrations of lead and chromium was used to study the effects of single lead and chromium stress or combination of them on the proline content in wheat seedlings and wheat seed germination. The results showed that the seed germination rate, seedling height, root length, fresh weight and dry biomass all decreased under lead and chromium stresses in both single and combination. The proline content in each treatment group increased comparing with that in the control group at 1 d after treatment, especially under combined 100 mg/L Pb +100 mg/L Cr stress, the proline content increased 32.81% compared with the control. The proline content in the wheat seedlings treated with 100 mg/L Cr, 50 mg/L Cr, 200 mg/L Pb +100 mg/L Cr and 100 mg/L Pb +100 mg/L Cr showed a significant increase by 3 d, 5 d, 7 d after treatment when compared to the untreated control. No significant changes were observed for proline content in the other treatment groups. In conclusion, lead and chromium stresses inhibited wheat seed germination and seedling growth, and increased proline content in wheat. Therefore, it is suggested that the proline content in wheat may be used as one of the indicators to monitor lead and chromium stress.

Key words: lead, chromium, seed germination, proline, toxic effect