关键词: 活性氧, 蛋白质, 翻译后修饰, 甲硫氨酸, 半胱氨酸

Abstract: Post-translational modification of protein is an important process for precursor protein to its carrying-out function. Some redox-sensitive proteins can be modified reversibly or irreversibly by reactive oxygen species in stress conditions. At present,research on oxidative modification of plants is still in the initial stage. With growing demand on plant developing the ability to defense the harsh environment,more attentions have been focused on the reversible oxidative modification of proteins caused by reactive oxygen species,and some corresponding progresses have been finished in its mechanism and research methods. This review describes the way and sites of oxidative modification of plant proteins and regulation mechanism of various oxidoreductase,aiming at illuminating the significance of reversible oxidative modification in the oxidative stress resulted from defensing the stress to plant. The review also summarizes the effective methods for studying the reversible oxidative modification of proteins. Currently,via mutations of specific sites and proteomics it is feasible to determine whether or not there are reversible oxidative modifications and in which extent the oxidative modification is. Moreover,in future,it is aimed to dissect the reduction and regeneration mechanism by comprehensive experiments to control the proteins involved in reactions.

Key words: reactive oxygen species, proteins, post-translational modification, methionine, cysteine