基于三螺旋核酸的生物传感器的研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2018-0381

摘要/Abstract

摘要： 三螺旋核酸是在经典的沃森-克里克（Waston-Crick）氢键形成的双链核酸基础上,第三条寡核苷酸链以非经典的胡斯特（Hoogsteen）氢键嵌入到双链大沟（Major groove）中形成的超分子核酸组装体。在近年来发展的众多生物传感方法中,基于三螺旋核酸的生物传感平台凭借其快速、灵敏、简单、可逆等特点而备受瞩目。从生物传感器的角度,综述了三螺旋核酸生物传感器的类别与性质,分类评述了常见的三螺旋核酸生物传感器与三螺旋核酸传感元件的应用,并对三螺旋核酸生物传感器的发展前景进行了展望。

关键词: 三螺旋核酸, 生物传感器, 评述

Abstract: A super-molecular nucleic acid assembly,termed Triplex Nucleic Acids（TNAs）,is formed between the third oligonucleotide strand and classical Waston-Crick base-pair-formed double strands through the non-classical Hoogsteen hydrogen bond at the major groove site. Among recently developed biosensors,TNAs-based sensing platforms have been attracting more attentions on account of the advantages of rapidity,simplicity,sensitivity,and reversibility. In this review,we generalized and exemplified different TNAs-based biosensors from their classifications,characteristics to applications. We also highlighted the development prospects of the TNAs biosensor.

Key words: triplex nucleic acid（TNAs）, biosensor, comment

田晶晶, 罗云波, 许文涛. 基于三螺旋核酸的生物传感器的研究进展[J]. 生物技术通报, 2018, 34(9): 15-28.

TIAN Jing-jing, LUO Yun-bo, XU Wen-tao. Advances in Biosensors Based on Triplex Nucleic Acids[J]. Biotechnology Bulletin, 2018, 34(9): 15-28.

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