水胁迫下沙漠小球藻蛋白质组学分析

doi:10.13560/j.cnki.biotech.bull.1985.2018-0423

摘要/Abstract

摘要： 通过研究小球藻（Chlorella sp.）在干旱胁迫下的蛋白质组变化,从蛋白质表达水平解释小球藻对干旱胁迫的响应机理。以20%的PEG 6000胁迫处理0、6、12、18、24和30 d,提取小球藻总蛋白,利用双向电泳和质谱鉴定技术分析差异蛋白。共鉴定29个差异蛋白,按功能可分为7类：光合作用、能量合成和转化、物质代谢、抗氧化、转运和细胞结构、抗逆和功能未知蛋白质。这些蛋白的表达变化影响着沙漠小球藻的油脂积累,对干旱胁迫应答起到了至关重要的作用,并直接或间接地参与了沙漠小球藻细胞内的光合作用和油脂合成。

关键词: 沙漠小球藻, 干旱胁迫, 蛋白质组, 差异表达蛋白

Abstract: By studying the proteomic changes of Chlorella under drought stress,the response mechanism of Chlorella to drought stress was explained from the level of protein expression. The total proteins of Chlorella treated with 20% PEG 6000 for 0,6,12,18,24,and 30 d were extracted. Two-dimensional electrophoresis and mass spectrometry techniques were applied to analyze the differential proteins. Totally 29 differential proteins were identified and classified into 7 categories based on function：photosynthesis,energy synthesis and transformation,substance metabolism,antioxidation,transport and cell structure,stress resistance,and unknown function proteins. The changes in the expressions of these proteins affect the accumulation of oil in the desert Chlorella,play a crucial role in the response to drought stress,and directly or indirectly are involved in the photosynthesis and oil synthesis in the cells of desert Chlorella.

Key words: desert Chlorella, drought stress, proteome, differentially expressed proteins

谭君, 牟云, 周光普, 周永顺, 徐杰, 高剑峰. 水胁迫下沙漠小球藻蛋白质组学分析[J]. 生物技术通报, 2018, 34(10): 207-216.

TAN Jun, MOU Yun, ZHOU Guang-pu, ZHOU Yong-shun, XU Jie, GAO Jian-feng. Proteomic Analysis of Desert Chlorella Under Drought Stress[J]. Biotechnology Bulletin, 2018, 34(10): 207-216.

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