单叶蔷薇NAC基因家族鉴定及干旱胁迫响应分析

doi:10.13560/j.cnki.biotech.bull.1985.2023-0531

摘要/Abstract

摘要：

NAC转录因子在植物胁迫应答中起关键作用，单叶蔷薇（Rosa persica）主要分布于我国新疆，研究其耐旱能力对治理荒漠地区生态问题具有重要意义。对单叶蔷薇NAC基因家族进行全基因组鉴定，分析基因家族特征和表达模式，利用RT-qPCR验证NAC基因在干旱胁迫下的表达情况。NAC基因家族的基因特征变化较大，而基因结构和基序相对保守。启动子分析表明RbeNAC在调节激素合成和适应环境胁迫方面具有重要作用。基于转录组数据分析发现RbeNAC基因的表达表现出组织特异性和对干旱胁迫的响应。实时荧光定量PCR（RT-qPCR）结果表明，7个基因（RbeATAF、RbeSOG1、RbeNAC17、RbeNAC71、RbeNAC72、RbeNAC90和RbeNAC96）在根和叶中积极响应干旱胁迫。本研究鉴定了单叶蔷薇NAC基因，初步探究了不同基因可能发挥的功能，获得了7个响应干旱胁迫的候选基因，为后续开展单叶蔷薇抗逆性育种提供参考依据。

关键词: 单叶蔷薇, NAC转录因子, 干旱胁迫, 基因表达

Abstract:

NAC transcription factors play a crucial role in plant stress response, and the study of the drought tolerance of Rosa persica, which is mainly distributed in Xinjiang Uygur Autonomous Region, China, is of great importance for the management of ecological problems in desert areas. Genome-wide identification of the NAC gene family in R. persica was performed, the gene family characteristics and expression patterns were analyzed, and the NAC gene expression under drought stress was validated using real time quantitative PCR（RT-qPCR）. The NAC gene family had a highly variable gene profile, while the gene structure and motifs were relatively conserved. Promoter analysis indicated that RbeNAC had an important role in the regulation of hormone synthesis and adaptation to environmental stresses. Analysis of transcriptome-based data revealed that RbeNAC gene expression demonstrated tissue specificity and response to drought stress. RT-qPCR results indicated that seven genes（RbeATAF, RbeSOG1, RbeNAC17, RbeNAC71, RbeNAC72, RbeNAC90 and RbeNAC96）in the roots and leaves, responded positively to drought stress. In this study, the NAC genes of R. persica were identified, the feasible functions of different genes were explored, and seven genes that respond positively to drought stress were found, which may provide a reference for breeding for stress resistance in R. persica.

Key words: Rosa persica, NAC transcription factors, drought stress, gene expression

冯策婷, 江律, 刘鑫颖, 罗乐, 潘会堂, 张启翔, 于超. 单叶蔷薇NAC基因家族鉴定及干旱胁迫响应分析[J]. 生物技术通报, 2023, 39(11): 283-296.

FENG Ce-ting, JIANG Lyu, LIU Xin-ying, LUO Le, PAN Hui-tang, ZHANG Qi-xiang, YU Chao. Identification of the NAC Gene Family in Rosa persica and Response Analysis Under Drought Stress[J]. Biotechnology Bulletin, 2023, 39(11): 283-296.

图/表 11

参考文献 40

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基因Gene	基因ID Gene ID	正向引物Forward primer（5'-3'）	反向引物序列Reverse primer（5'-3'）
GAPDH		ATCCATTCATCACCACCGACTACA	GCATCCTTACTTGGGGCAGAGA
RbeNAC71	Rbe024972	TGAAATGAATAAAGAGGCACAGGG	GCAACGCATTACAGTCACCA
RbeNAC89	Rbe024974	ATTCCAGCCACAAGATTCCACA	TTGAAAGGAGTCTGTGCTGTTTG
RbeNAC90	Rbe028302	AGACGACCTTCAGGCATTGG	TTGGAGCAGCTGGGATGATG
RbeATAF	Rbe007404	TTCGATCCGTGGCAGTTACC	CAATGTGCTTGTCTGCTCCG
RbeNAC72	Rbe002086	ACAGGTTCTTCACTCTGCCG	CTTGTCCTTGGGCTTGGGTA
RbeSOG1	Rbe021851	ACACTGGAACTCGAAAGCGT	TCTCAGCTTTGCCGCCTTTA
RbeNAC17	Rbe011929	TGGGTCCTGAACCTTCAAACA	TGGAGGTGCTGGTTGTATCTG
RbeNAC53	Rbe023720	GCCTGACGTGAAATTTGGACC	ATCATGCCAGCACGAACTGA
RbeNAC96	Rbe010166	TGGGAACATGGATCGAGGGA	TCGGATGAGCGCTTGTTCTT

基因Gene	基因ID Gene ID	正向引物Forward primer（5'-3'）	反向引物序列Reverse primer（5'-3'）
GAPDH		ATCCATTCATCACCACCGACTACA	GCATCCTTACTTGGGGCAGAGA
RbeNAC71	Rbe024972	TGAAATGAATAAAGAGGCACAGGG	GCAACGCATTACAGTCACCA
RbeNAC89	Rbe024974	ATTCCAGCCACAAGATTCCACA	TTGAAAGGAGTCTGTGCTGTTTG
RbeNAC90	Rbe028302	AGACGACCTTCAGGCATTGG	TTGGAGCAGCTGGGATGATG
RbeATAF	Rbe007404	TTCGATCCGTGGCAGTTACC	CAATGTGCTTGTCTGCTCCG
RbeNAC72	Rbe002086	ACAGGTTCTTCACTCTGCCG	CTTGTCCTTGGGCTTGGGTA
RbeSOG1	Rbe021851	ACACTGGAACTCGAAAGCGT	TCTCAGCTTTGCCGCCTTTA
RbeNAC17	Rbe011929	TGGGTCCTGAACCTTCAAACA	TGGAGGTGCTGGTTGTATCTG
RbeNAC53	Rbe023720	GCCTGACGTGAAATTTGGACC	ATCATGCCAGCACGAACTGA
RbeNAC96	Rbe010166	TGGGAACATGGATCGAGGGA	TCGGATGAGCGCTTGTTCTT

基因ID Gene ID	长度 Length/aa	等电点 pI	分子量 MW/Da	基因ID Gene ID	长度 Length/aa	等电点 pI	分子量 MW/Da
Rbe000437	238	9.50	26742.40	Rbe015425	376	6.67	42077.99
Rbe000577	342	7.78	38395.04	Rbe015545	399	5.81	45802.91
Rbe001198	307	8.95	35352.84	Rbe015576	732	5.11	82140.75
Rbe001377	362	7.02	41371.16	Rbe015696	231	8.84	26081.39
Rbe001406	347	4.97	39490.91	Rbe016577	346	5.96	39956.38
Rbe001560	322	8.05	36896.91	Rbe016791	197	4.87	22500.07
Rbe001607	291	8.11	32702.45	Rbe016986	368	5.39	42000.66
Rbe001675	97	6.90	11254.85	Rbe017102	443	4.59	49942.32
Rbe001680	252	5.82	29423.90	Rbe017312	223	8.43	25796.15
Rbe001684	181	9.94	21272.39	Rbe017370	348	4.76	39766.49
Rbe001687	213	5.63	24822.67	Rbe017374	431	4.63	49283.85
Rbe001793	458	4.86	52053.89	Rbe017385	444	4.55	50806.24
Rbe001840	357	5.06	41560.98	Rbe017419	403	6.36	44863.19
Rbe001892	410	4.44	47117.37	Rbe017564	414	6.55	47093.99
Rbe001938	360	8.09	40478.45	Rbe017971	197	4.73	22842.29
Rbe001942	413	4.44	47485.04	Rbe018482	338	8.70	38084.18
Rbe002086	360	8.64	40516.77	Rbe019654	172	7.82	19889.40
Rbe002090	342	7.77	38420.98	Rbe021391	251	9.07	28538.35
Rbe002324	310	5.57	36546.13	Rbe021851	458	5.08	51616.50
Rbe002753	476	4.30	54814.79	Rbe022037	201	8.59	22769.20
Rbe002755	353	4.41	40705.39	Rbe022113	287	8.63	32863.08
Rbe002766	258	7.70	29401.71	Rbe022146	457	6.70	50844.68
Rbe002769	254	6.46	28883.99	Rbe023180	404	4.92	45588.37
Rbe002801	383	6.68	43138.51	Rbe023181	484	4.91	55066.39
Rbe003148	547	5.44	62129.20	Rbe023665	484	4.68	54311.74
Rbe003554	346	6.68	39376.24	Rbe023667	411	5.68	47110.37
Rbe005498	495	6.65	55920.03	Rbe023679	152	4.50	17723.53
Rbe006018	362	5.06	41346.93	Rbe023719	621	5.81	67734.40
Rbe006711	135	7.67	15864.06	Rbe023720	593	4.63	66279.49
Rbe007404	315	6.55	36127.66	Rbe024330	314	6.17	36186.69
Rbe007596	577	5.40	64238.73	Rbe024403	583	4.65	66052.22
Rbe007658	379	8.97	41903.52	Rbe024932	420	5.93	47167.13
Rbe008463	443	8.48	49552.51	Rbe024933	348	5.97	39746.40
Rbe008464	224	8.22	24996.02	Rbe024970	610	4.74	68426.60
Rbe008978	334	8.05	38419.03	Rbe024971	509	4.62	56551.30
Rbe009057	364	4.56	41960.70	Rbe024972	309	5.75	34860.02
Rbe009768	193	7.80	22531.76	Rbe024973	248	4.67	27472.93
Rbe009867	335	6.34	38111.56	Rbe024974	525	5.44	60098.24
Rbe009920	363	7.00	40900.71	Rbe024976	576	5.06	64862.49
Rbe010166	251	9.17	29202.10	Rbe024978	618	4.44	68573.07
Rbe010181	404	5.53	45245.49	Rbe024980	475	4.75	53362.64
Rbe010351	281	9.59	32122.23	Rbe024981	451	5.11	50390.38
Rbe010428	406	6.68	46160.96	Rbe024984	477	6.08	53897.88
Rbe011929	592	4.88	66676.94	Rbe024986	325	6.09	37252.33
Rbe012001	382	5.16	42689.74	Rbe025006	348	6.07	39640.32
Rbe012002	442	4.69	49961.94	Rbe025809	333	6.31	38592.32
Rbe012071	344	4.39	40039.95	Rbe026285	567	5.09	63339.26
Rbe012355	309	6.11	35776.03	Rbe026837	241	5.85	27548.43
Rbe012668	577	4.81	66799.58	Rbe027180	296	5.97	34483.57
Rbe012863	285	5.61	32829.91	Rbe027926	357	8.32	40962.22
Rbe013487	290	7.58	33044.33	Rbe028089	498	5.57	55555.28
Rbe013835	377	8.17	41907.56	Rbe028302	291	6.61	32585.37
Rbe014245	350	8.92	39026.85	Rbe028714	654	5.59	76078.14
Rbe014438	279	7.10	31333.13	Rbe028715	172	8.56	20262.10
Rbe014843	99	4.43	11155.44	Rbe028783	429	7.72	48014.20
Rbe014844	321	7.57	36154.30	Rbe029101	687	5.64	78005.28
Rbe015173	353	7.12	40042.08	Rbe029577	465	6.07	51857.88
Rbe015176	590	6.06	66049.28	Rbe029972	395	7.13	44952.76
Rbe015232	171	7.77	19860.37	Rbe030070	433	4.51	48946.22

基因ID Gene ID	长度 Length/aa	等电点 pI	分子量 MW/Da	基因ID Gene ID	长度 Length/aa	等电点 pI	分子量 MW/Da
Rbe000437	238	9.50	26742.40	Rbe015425	376	6.67	42077.99
Rbe000577	342	7.78	38395.04	Rbe015545	399	5.81	45802.91
Rbe001198	307	8.95	35352.84	Rbe015576	732	5.11	82140.75
Rbe001377	362	7.02	41371.16	Rbe015696	231	8.84	26081.39
Rbe001406	347	4.97	39490.91	Rbe016577	346	5.96	39956.38
Rbe001560	322	8.05	36896.91	Rbe016791	197	4.87	22500.07
Rbe001607	291	8.11	32702.45	Rbe016986	368	5.39	42000.66
Rbe001675	97	6.90	11254.85	Rbe017102	443	4.59	49942.32
Rbe001680	252	5.82	29423.90	Rbe017312	223	8.43	25796.15
Rbe001684	181	9.94	21272.39	Rbe017370	348	4.76	39766.49
Rbe001687	213	5.63	24822.67	Rbe017374	431	4.63	49283.85
Rbe001793	458	4.86	52053.89	Rbe017385	444	4.55	50806.24
Rbe001840	357	5.06	41560.98	Rbe017419	403	6.36	44863.19
Rbe001892	410	4.44	47117.37	Rbe017564	414	6.55	47093.99
Rbe001938	360	8.09	40478.45	Rbe017971	197	4.73	22842.29
Rbe001942	413	4.44	47485.04	Rbe018482	338	8.70	38084.18
Rbe002086	360	8.64	40516.77	Rbe019654	172	7.82	19889.40
Rbe002090	342	7.77	38420.98	Rbe021391	251	9.07	28538.35
Rbe002324	310	5.57	36546.13	Rbe021851	458	5.08	51616.50
Rbe002753	476	4.30	54814.79	Rbe022037	201	8.59	22769.20
Rbe002755	353	4.41	40705.39	Rbe022113	287	8.63	32863.08
Rbe002766	258	7.70	29401.71	Rbe022146	457	6.70	50844.68
Rbe002769	254	6.46	28883.99	Rbe023180	404	4.92	45588.37
Rbe002801	383	6.68	43138.51	Rbe023181	484	4.91	55066.39
Rbe003148	547	5.44	62129.20	Rbe023665	484	4.68	54311.74
Rbe003554	346	6.68	39376.24	Rbe023667	411	5.68	47110.37
Rbe005498	495	6.65	55920.03	Rbe023679	152	4.50	17723.53
Rbe006018	362	5.06	41346.93	Rbe023719	621	5.81	67734.40
Rbe006711	135	7.67	15864.06	Rbe023720	593	4.63	66279.49
Rbe007404	315	6.55	36127.66	Rbe024330	314	6.17	36186.69
Rbe007596	577	5.40	64238.73	Rbe024403	583	4.65	66052.22
Rbe007658	379	8.97	41903.52	Rbe024932	420	5.93	47167.13
Rbe008463	443	8.48	49552.51	Rbe024933	348	5.97	39746.40
Rbe008464	224	8.22	24996.02	Rbe024970	610	4.74	68426.60
Rbe008978	334	8.05	38419.03	Rbe024971	509	4.62	56551.30
Rbe009057	364	4.56	41960.70	Rbe024972	309	5.75	34860.02
Rbe009768	193	7.80	22531.76	Rbe024973	248	4.67	27472.93
Rbe009867	335	6.34	38111.56	Rbe024974	525	5.44	60098.24
Rbe009920	363	7.00	40900.71	Rbe024976	576	5.06	64862.49
Rbe010166	251	9.17	29202.10	Rbe024978	618	4.44	68573.07
Rbe010181	404	5.53	45245.49	Rbe024980	475	4.75	53362.64
Rbe010351	281	9.59	32122.23	Rbe024981	451	5.11	50390.38
Rbe010428	406	6.68	46160.96	Rbe024984	477	6.08	53897.88
Rbe011929	592	4.88	66676.94	Rbe024986	325	6.09	37252.33
Rbe012001	382	5.16	42689.74	Rbe025006	348	6.07	39640.32
Rbe012002	442	4.69	49961.94	Rbe025809	333	6.31	38592.32
Rbe012071	344	4.39	40039.95	Rbe026285	567	5.09	63339.26
Rbe012355	309	6.11	35776.03	Rbe026837	241	5.85	27548.43
Rbe012668	577	4.81	66799.58	Rbe027180	296	5.97	34483.57
Rbe012863	285	5.61	32829.91	Rbe027926	357	8.32	40962.22
Rbe013487	290	7.58	33044.33	Rbe028089	498	5.57	55555.28
Rbe013835	377	8.17	41907.56	Rbe028302	291	6.61	32585.37
Rbe014245	350	8.92	39026.85	Rbe028714	654	5.59	76078.14
Rbe014438	279	7.10	31333.13	Rbe028715	172	8.56	20262.10
Rbe014843	99	4.43	11155.44	Rbe028783	429	7.72	48014.20
Rbe014844	321	7.57	36154.30	Rbe029101	687	5.64	78005.28
Rbe015173	353	7.12	40042.08	Rbe029577	465	6.07	51857.88
Rbe015176	590	6.06	66049.28	Rbe029972	395	7.13	44952.76
Rbe015232	171	7.77	19860.37	Rbe030070	433	4.51	48946.22