全基因组小麦PPR基因家族鉴定及表达分析

doi:10.13560/j.cnki.biotech.bull.1985.2019-0165

摘要/Abstract

摘要： 旨在筛选对二系杂交小麦杂交种具有育性恢复功能的PPR（Pentatricopeptide repeats）基因,以期进一步研究PPR基因的功能及调控机制。通过对小麦全基因组数据进行生物信息学分析,获得了1 351个小麦PPR成员,并对该基因家族进行了染色体定位与分布分析及进化树构建。通过与已报道的水稻育性恢复基因进行同源比对分析,候选了23个育性恢复相关PPR基因。进一步对高、低2种不同类型恢复系、不育系及杂交种减数分裂期幼穗取样,对23个育性恢复相关PPR基因进行实时荧光定量PCR表达模式筛选鉴定。结合结实率等重要农艺性状表型分析发现,4个PPR基因在高恢复系杂交组合中表现出了明显的超亲表达模式,相应地,这4个基因在低恢复系杂交组合中基本呈现低亲的表达模式。初步确认了4个可能参与调控二系杂交小麦杂交种的育性恢复基因。

关键词: 小麦, PPR基因, 同源比对, 育性恢复

Abstract: The aim is to screen the PPR（Pentatricopeptide repeats）gene with fertility restoration function for the hybrids of the two-line hybrid wheat,and to further study the function and regulation mechanism of PPR genes. After bioinformatics analysis of wheat genome-wide data,1 351 PPR members were obtained. Then,the chromosome location and distribution of PPR gene family in wheat were analyzed,and the phylogenetic tree was constructed. Total 23 PPR genes related to fertility restoration were obtained by homology align and analysis of them with reported fertility restorer genes in rice. Real-time fluorescence quantitative PCR was performed to screen and identify the expression pattern of 23 fertility restoration genes on young spikes of 2 different restorer lines of high and low,one sterile line,and their F₁ generation in two-line hybrid wheat at meiotic stage. Based on phenotypic analysis of important agronomic traits such as seed setting rate,it was found that the 4 PPR genes showed obvious over-dominance patterns in the high-restorer hybrid combinations,and correspondingly these 4 genes basically presented low-parent dominance expression patterns in low-restorer line hybrid combinations. It is preliminarily confirmed that these 4 PPR genes may be involved in the regulation of fertility restoration of the two-line hybrid wheat.

Key words: wheat, PPR gene family, homology align, fertility restoration

郭彩娟, 公杰, 刘永杰, 赵昌平, 高世庆, 吴华伟. 全基因组小麦PPR基因家族鉴定及表达分析[J]. 生物技术通报, 2019, 35(8): 1-8.

GUO Cai-juan, GONG Jie, LIU Yong-jie, ZHAO Chang-ping, GAO Shi-qing, WU Hua-wei. Genome-wide Bioinformatics Identification of PPR Gene Family and Expression Profiles Analysis in Wheat[J]. Biotechnology Bulletin, 2019, 35(8): 1-8.

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