宁夏中宁地区灵武长枣果实多糖的单糖成分GC-MS分析

doi:10.13560/j.cnki.biotech.bull.1985.2019-0304

摘要/Abstract

摘要： 研究宁夏中宁地区灵武长枣果实多糖的单糖成分,为不同地区灵武长枣的开发利用提供试验依据。以不同发育时期中宁产灵武长枣果实为试验材料,采用热水浸提法和气相色谱-质谱联用法（GC-MS）等,探讨不同发育时期果实多糖含量变化规律及其单糖组成成分。结果表明,不同发育时期中宁产灵武长枣果实粗多糖得率分别为：膨大前期0.43%、快速膨大期0.527%、着色期0.80%和完熟期0.618%;果实粗多糖经DEAE-52均得到Ju-0、Ju-1、Ju-2、Ju-3 4个多糖级分,其中Ju-2含量为最高,表明果实多糖的主要形式为酸性多糖;不同发育时期果实各精制多糖总共含有阿拉伯糖、鼠李糖、核糖、岩藻糖、木糖、甘露糖、半乳糖、葡萄糖、葡萄糖醛酸、半乳糖醛酸10种单糖,不含果糖,以阿拉伯糖、半乳糖、核糖、鼠李糖、糖醛酸含量较高,岩藻糖、葡萄糖含量次之,木糖、甘露糖含量较低,但其含量各组分有差异。精制多糖含量随着果实的发育进程总体呈现上升趋势;不同发育时期中宁产灵武长枣果实各精制多糖中单糖组成及含量各不相同。

关键词: 中宁, 灵武长枣, 果实, 多糖, 单糖组成, GC-MS

Abstract: The monosaccharide composition of polysaccharides in Ziziphus jujuba Mill cv.lingwuchangzao fruit from Zhongning areas in Ningxia was studied to provide experimental basis for the development and utilization of different areas Ziziphus jujuba Mill cv.lingwuchangzao. The fruit of Ziziphus jujuba Mill cv.lingwuchangzao from Zhongning areas in different developmental periods was used as experimental material to research the change rules of polysaccharides content and monosaccharide composition by the methods of hot water extraction and gas chromatography-mass spectrometry（GC-MS）. The main research results are followed：the crude polysaccharides obtaining ratio in Ziziphus jujuba Mill cv. lingwuchangzao fruits from Zhongning areas in different developmental periods is respectively 0.43% in the early bulking period,0.527% in the rapid enlargement period,0.80% in the coloring period,and 0.618% in the fruit maturation period. The crude polysaccharide in different developmental periods fruit was fractioned respectively to four fractions（Ju-0,Ju-1,Ju-2,Ju-3）by DEAE-52 cellulose ion-exchange columns,and Ju-2 had the highest contents,this indicated that the main components of polysaccharides in fruit was acid fractions. Finally,the refined polysaccharides in the different developmental periods fruit were analysed by the GC-MS,the results showed that polysaccharides were composed of ten monosaccharides：arabinose,rhamnose,ribose,fucose,xylose,mannose,galactose,glucose,glucuronic acid and galacturonic acid,and did not contain fructose,the contents of arabinose,galactose,ribose,rhamnose,glucuronic acid and galacturonic acid were higher,followed by the fucose and glucose,and the contents of xylose and mannose were the lowest,but the monosaccharide composition and contents are different in different polysaccharides component. Conclusion,The refined polysaccharide contents present ascendant trend with the development of the fruits,the monosaccharide composition and contents of the refined polysaccharide of Ziziphus jujuba Mill cv.lingwuchangzao fruits from Zhongning areas in different developmental periods are different respectively in different polysaccharides component.

Key words: Zhongning areas, Ziziphus jujuba Mill cv. Lingwuchangzao, fruit, polysaccharides, monosaccharide composition, GC-MS

章英才, 苏伟东, 柴雅红. 宁夏中宁地区灵武长枣果实多糖的单糖成分GC-MS分析[J]. 生物技术通报, 2019, 35(11): 22-29.

ZHANG Ying-cai, SU Wei-dong, CHAI Ya-hong. Analyse on Monosaccharide Composition of Polysaccharides in Ziziphus jujuba Mill cv. lingwuchangzao Fruit from Zhongning Areas in Ningxia by GC-MS[J]. Biotechnology Bulletin, 2019, 35(11): 22-29.

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