烟草过氧化物酶基因NtPOD2的克隆及表达分析

doi:10.13560/j.cnki.biotech.bull.1985.2019-0395

摘要/Abstract

摘要： 过氧化物酶（Peroxidase,POD）存在于植物的各个器官,对植物生长发育以及逆境胁迫起着重要作用。为探究烟草中POD基因家族的功能,通过基因克隆方法,从普通烟草品种K326中克隆到一个过氧化物酶基因,该基因与绒毛状烟草（Nicotiana tomentosiformis）POD2具有99%同源性,故命名为NtPOD2。利用生物信息学分析来预测NtPOD2的结构,qRT-PCR技术来测定NtPOD2在不同组织中以及非生物逆境胁迫下的表达量。结果表明,该基因序列包含了一个897 bp的开放阅读框,编码由298个氨基酸残基,NtPOD2蛋白具有Ⅲ型过氧化物酶典型保守结构域。NtPOD2在根、茎、叶、花中都有表达,但在根中表达量最高,且能快速响应非生物胁迫诱导。NtPOD2可能参与了植物生长发育以及非生物逆境胁迫。

关键词: 烟草, NtPOD2, 克隆, 表达

Abstract: Peroxidase（POD）is widely present in various organs and different developmental stages of plants,and plays an important role in plant growth,development and coping with stress. In order to explore the function of POD gene family in tobacco,A peroxidase gene was cloned from the common tobacco K326 by gene cloning. The gene was 99% homologous of Nicotiana tomentosiformis POD2,thus it was named NtPOD2 gene. Bioinformatics analysis was used to predict the structure of the NtPOD2 gene,and qRT-PCR technique to determine the expression level of NtPOD2 in different tissues and abiotic stress. Results showed that the sequence contained an open reading frame（ORF）of 897 bp and encoded 298 amino acid residues,and NtPOD2 protein had a type Ⅲ peroxidase typical conserved domain. NtPOD2 gene was expressed in root,stem,leave and flower,but the highest expression in root,and rapidly responded to abiotic stress induction. This indicates that NtPOD2 may be involved in plant growth,development and abiotic stress.

Key words: tobacco, NtPOD2, cloning, expression

王静, 刘仑, 杨勇, 胡圣, 李立芹. 烟草过氧化物酶基因NtPOD2的克隆及表达分析[J]. 生物技术通报, 2019, 35(12): 24-30.

WANG Jing, LIU Lun, YANG Yong, HU Sheng, LI Li-qin. Cloning and Expression Analysis of Tobacco Peroxidase Gene NtPOD2[J]. Biotechnology Bulletin, 2019, 35(12): 24-30.

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