植物Dof基因结构特点及功能研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2020-0112

摘要/Abstract

摘要：

DNA结合单锌指（DNA binding with one zinc finger,Dof）蛋白是由多基因共同编码的只在植物中存在的一类转录因子,其N-末端含有高度保守的由52个氨基酸残基组成的C2-C2型单锌指结构域,可以与DNA和蛋白质结合并相互作用;C-末端是特异转录调控结构域,因其序列具有多变性,导致Dof基因功能的多样性。随着基因组学和生物信息学的发展,近年来已有多个物种的Dof蛋白相继被报道出来。Dof基因主要参与植物的生长发育、碳氮代谢、非生物胁迫及开花调控等生物学过程。综述了植物Dof转录因子的结构特性、已经报道的Dof基因数目及生物学功能,在此基础上讨论了毛竹开花研究存在的问题,并提出未来的展望,以期为进一步研究Dof转录因子提供参考。

关键词: 植物, Dof转录因子, 结构特点, 功能研究

Abstract:

DNA binding with one zinc finger（Dof）is a kind of specific transcription factors only in plant,which are encoded by multiple genes. The N-terminus contains a highly-conserved C2-C2 single zinc finger domain consisting of 52 amino acid residues,which could bind and interact with DNA and proteins. The C-terminus is a specific transcriptional regulatory domain,its variable amino acid sequence results in the functional diversity of Dof genes. With the development of genomics and bioinformatics,Dof proteins of multiple species have been reported in recent years. The Dof genes are mainly involved in biological processes,such as plant growth and development,carbon and nitrogen metabolism,abiotic stress,and flowering regulation. Hereby we review the structural characteristics of Dof transcription factors,the number of reported Dof genes and their biological functions. We also discuss the current issues and the future prospects in studying moso bamboo flowering,aiming to provide a reference for the further study of Dof transcription factors.

Key words: plants, Dof transcription factor, structural characteristics, function research

刘俊, 金钰, 吴耀松, 刘燕, 王文彬, 任闪闪, 刁松锋, 陈玉龙. 植物Dof基因结构特点及功能研究进展[J]. 生物技术通报, 2020, 36(10): 180-190.

LIU Jun, JIN Yu, WU Yao-song, LIU Yan, WANG Wen-bin, REN Shan-shan, DIAO Song-feng, CHEN Yu-long. Advances on the Structural Characteristics and Function of Dof Gene in Plant[J]. Biotechnology Bulletin, 2020, 36(10): 180-190.

图/表 1

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物种	拉丁名	分类	总数	参考文献
水稻	Oryza sativa	单子叶植物	30	[5]
毛竹	Phyllostachys edulis	单子叶植物	26	[6]
玉米	Zea mays	单子叶植物	54	[7]
高粱	Sorghum bicolor	单子叶植物	28	[8]
大麦	Hordeum vuLgare	单子叶植物	24	[9]
小麦	Triticumaestivum	单子叶植物	31	[10]
二穗短柄草	Brachypodium distachyum	单子叶植物	27	[11]
甘蔗	Saccharum officinarum	单子叶植物	25	[8]
香蕉	Musa acuminate	单子叶植物	72	[12]
穇子	Eleusine coracana	单子叶植物	48	[13]
粗山羊草	Aegilops tauschii	单子叶植物	10	[14]
拟南芥	Arabidopsis thaliana	双子叶植物	36	[15]
毛果杨	PopuLus trichocarpa	双子叶植物	41	[16]
番茄	Solanum lycopersicum	双子叶植物	34	[17]
木豆	Cajanus cajan	双子叶植物	38	[18]
土豆	Solanumtuberosum	双子叶植物	35	[19]
葡萄	Vitis vinifera	双子叶植物	25	[20]
花生	Arachis hypogaea	双子叶植物	8	[21]
茄子	Solanum melongena	双子叶植物	29	[22]
黄瓜	Cucumis sativus	双子叶植物	36	[23]
苹果	Malus domestica	双子叶植物	60	[24]
胡萝卜	Daucus carota	双子叶植物	46	[25]
大白菜	Chinese cabbage	双子叶植物	76	[26]
大豆	Glycine max	双子叶植物	78	[27]
菊花	Dendranthema morifolium	双子叶植物	20	[28]
马铃薯	Solanum tuberosum	双子叶植物	35	[19]
胡椒	Capsicum annuum L.	双子叶植物	33	[29]
茶树	Camellia sinensis	双子叶植物	29	[30]
蒺藜苜蓿	Medicago truncatula	双子叶植物	42	[31]
木薯	Manihot esculenta Crantz	双子叶植物	45	[32]
西瓜	Citrullus lanatus	双子叶植物	39	[33]
蓖麻	Ricinus communis	双子叶植物	24	[34]
麻风树	Jatropha curcas	双子叶植物	25	[34]
榴莲	Durio zibethinus Murr.	双子叶植物	24	[35]
梨	Pyrus bretschneideri	双子叶植物	45	[36]
火炬松	Pinus taeda	松杉	8	[9]
莱茵衣藻	Chlamydomonas reinhardti	藻类	1	[9]
小立碗藓	Physcomitrella patens	苔藓	23	[29]

物种	拉丁名	分类	总数	参考文献
水稻	Oryza sativa	单子叶植物	30	[5]
毛竹	Phyllostachys edulis	单子叶植物	26	[6]
玉米	Zea mays	单子叶植物	54	[7]
高粱	Sorghum bicolor	单子叶植物	28	[8]
大麦	Hordeum vuLgare	单子叶植物	24	[9]
小麦	Triticumaestivum	单子叶植物	31	[10]
二穗短柄草	Brachypodium distachyum	单子叶植物	27	[11]
甘蔗	Saccharum officinarum	单子叶植物	25	[8]
香蕉	Musa acuminate	单子叶植物	72	[12]
穇子	Eleusine coracana	单子叶植物	48	[13]
粗山羊草	Aegilops tauschii	单子叶植物	10	[14]
拟南芥	Arabidopsis thaliana	双子叶植物	36	[15]
毛果杨	PopuLus trichocarpa	双子叶植物	41	[16]
番茄	Solanum lycopersicum	双子叶植物	34	[17]
木豆	Cajanus cajan	双子叶植物	38	[18]
土豆	Solanumtuberosum	双子叶植物	35	[19]
葡萄	Vitis vinifera	双子叶植物	25	[20]
花生	Arachis hypogaea	双子叶植物	8	[21]
茄子	Solanum melongena	双子叶植物	29	[22]
黄瓜	Cucumis sativus	双子叶植物	36	[23]
苹果	Malus domestica	双子叶植物	60	[24]
胡萝卜	Daucus carota	双子叶植物	46	[25]
大白菜	Chinese cabbage	双子叶植物	76	[26]
大豆	Glycine max	双子叶植物	78	[27]
菊花	Dendranthema morifolium	双子叶植物	20	[28]
马铃薯	Solanum tuberosum	双子叶植物	35	[19]
胡椒	Capsicum annuum L.	双子叶植物	33	[29]
茶树	Camellia sinensis	双子叶植物	29	[30]
蒺藜苜蓿	Medicago truncatula	双子叶植物	42	[31]
木薯	Manihot esculenta Crantz	双子叶植物	45	[32]
西瓜	Citrullus lanatus	双子叶植物	39	[33]
蓖麻	Ricinus communis	双子叶植物	24	[34]
麻风树	Jatropha curcas	双子叶植物	25	[34]
榴莲	Durio zibethinus Murr.	双子叶植物	24	[35]
梨	Pyrus bretschneideri	双子叶植物	45	[36]
火炬松	Pinus taeda	松杉	8	[9]
莱茵衣藻	Chlamydomonas reinhardti	藻类	1	[9]
小立碗藓	Physcomitrella patens	苔藓	23	[29]