ERF转录因子调控生物胁迫反应的研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2020-0736

摘要/Abstract

摘要：

ERF家族是植物所特有的APETALA2 / 乙烯响应因子（APETALA2 / ethylene-responsive factor,AP2 / ERF）转录因子家族的一个主要亚家族,其成员结构特点是仅含有 1个58-60 个氨基酸组成的AP2 / ERF结构域。有关该家族成员的大多数研究集中在与寒、旱等非生物胁迫方面,最近越来越多的研究表明ERF在植物抵御病虫侵害等生物胁迫方面也发挥着重要作用。ERF亚家族成员通过结合下游互作基因启动子区域的GCC box元件,从而激活或抑制这些病程相关基因的表达。同时ERFs参与水杨酸（salicylic acid,SA）、茉莉酸（jasmonic acid,JA）、乙烯（ethylene,ET）及过氧化氢（H₂O₂）等多种激素的信号途径,通过相互促进/拮抗高效协调体内不同激素抵御病原菌的入侵,提高植物的抗病、抗虫性。本文综述了ERF 转录因子的结构功能特点、在不同植物抗生物胁迫中的调控方式,以及其通过协调不同激素信号途径相互作用来提高植物抗病虫的最新研究进展,并对其应用前景进行了展望。

关键词: ERF转录因子, 传导途径, 生物胁迫, 病虫害

Abstract:

ERF family is a major subfamily of plant-specific APETALA2/ethylene-responsive factor（AP2/ERF）transcription factor family. Its member structure is characterized by containing only one AP2/ERF domain composed of 58-60 amino acids. Most researches on the members of this family were focused on abiotic stresses such as cold and drought,and recent studies demonstrate that ERFs play an important role in resisting plant diseases and insect pests. These ERF subfamily members can bind to the GCC box element within the promoter region of their interactive genes,and activate or inhibit the expressions of these pathogenesis-related genes. Moreover,more researches confirmed that ERFs participate in salicylic acid（SA）,jasmonic acid（JA）,ethylene（ET）,H₂O_2,and other hormone signaling pathways,and effectively coordinate different hormones to resist the invasion of pathogenic bacteria,and to improve plants’ resistance to disease and insect through mutual promotion/antagonism. This paper reviews the structure features of ERF transcription factors（TF）,their regulatory mechanisms in different plants’ antibiotic stresses,and the latest progresses in improving plant resistance to diseases and insect pests by coordinating the interaction of different hormone signaling pathways. Finally,the paper prospects the application of ERF in plant disease resistance breeding.

Key words: ERF transcription factor, transduction pathway, biological stress, diseases and pests

邵文靖, 石洁, 张普, 郎明林. ERF转录因子调控生物胁迫反应的研究进展[J]. 生物技术通报, 2021, 37(3): 136-143.

SHAO Wen-jing, SHI Jie, ZHANG Pu, LANG Ming-lin. Research Progress of ERF Transcription Factors in Regulating Biological Stress Responses[J]. Biotechnology Bulletin, 2021, 37(3): 136-143.

图/表 3

参考文献 51

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ERF基因	基因来源	转基因植物	调控模式	基因功能	参考文献
ERF1	拟南芥	拟南芥	正调控	增强灰霉菌的抗性	[37]
ORA59	拟南芥	拟南芥	正调控	增强油菜链格孢菌和灰霉菌的抗性	[30]
OsERF83	水稻	水稻	正调控	增强稻瘟病的抗性	[36]
OsERF96	水稻	水稻	正调控	增强白叶枯病或稻瘟病的抗性	[43]
OsERF922	水稻	水稻	负调控	沉默基因增强稻瘟病抗性	[32]
OsERF7	水稻	水稻	负调控	沉默基因增强褐飞虱和白背飞虱抗性	[31]
SlERF.D.3	番茄	番茄	正调控	增强灰霉菌抗性	[44]
SlERF.A1、SlERF.A3、SlERF.B4、SlERF.C3	番茄	番茄	正调控	增强灰霉菌和丁香假单胞菌细菌的抗性	[8]
ERF-E2	番茄	番茄	正调控	增强烟草根节线虫和马铃薯白线虫的抗性	[42]
ERF19	番茄	番茄	负调控	增强灰霉菌和丁香假单胞菌细菌的抗性	[33]
GhERFB101	棉花	棉花	正调控	增强棉花抗枯萎病的抗性	[41]
Pdt-ERF3、Pnd-ERF3	杨树	杂交杨	正调控	增强落叶松-杨栅锈菌抗性	[39]
MdERF113	苹果	苹果*	正调控	增强轮纹病菌	[45]
VaERF20	葡萄	拟南芥	正调控	增强灰霉菌和丁香假单胞菌细菌的抗性	[40]
VqERF112、VqERF114、VqERF072	葡萄	拟南芥	正调控	增强灰霉菌和丁香假单胞菌细菌的抗性	[35]
Sm ORA1	丹参	丹参	正调控	增强抗立枯丝核菌	[46]
StERF94	马铃薯	马铃薯	正调控	增强茄枯萎病的抗性	[27]

ERF基因	基因来源	转基因植物	调控模式	基因功能	参考文献
ERF1	拟南芥	拟南芥	正调控	增强灰霉菌的抗性	[37]
ORA59	拟南芥	拟南芥	正调控	增强油菜链格孢菌和灰霉菌的抗性	[30]
OsERF83	水稻	水稻	正调控	增强稻瘟病的抗性	[36]
OsERF96	水稻	水稻	正调控	增强白叶枯病或稻瘟病的抗性	[43]
OsERF922	水稻	水稻	负调控	沉默基因增强稻瘟病抗性	[32]
OsERF7	水稻	水稻	负调控	沉默基因增强褐飞虱和白背飞虱抗性	[31]
SlERF.D.3	番茄	番茄	正调控	增强灰霉菌抗性	[44]
SlERF.A1、SlERF.A3、SlERF.B4、SlERF.C3	番茄	番茄	正调控	增强灰霉菌和丁香假单胞菌细菌的抗性	[8]
ERF-E2	番茄	番茄	正调控	增强烟草根节线虫和马铃薯白线虫的抗性	[42]
ERF19	番茄	番茄	负调控	增强灰霉菌和丁香假单胞菌细菌的抗性	[33]
GhERFB101	棉花	棉花	正调控	增强棉花抗枯萎病的抗性	[41]
Pdt-ERF3、Pnd-ERF3	杨树	杂交杨	正调控	增强落叶松-杨栅锈菌抗性	[39]
MdERF113	苹果	苹果*	正调控	增强轮纹病菌	[45]
VaERF20	葡萄	拟南芥	正调控	增强灰霉菌和丁香假单胞菌细菌的抗性	[40]
VqERF112、VqERF114、VqERF072	葡萄	拟南芥	正调控	增强灰霉菌和丁香假单胞菌细菌的抗性	[35]
Sm ORA1	丹参	丹参	正调控	增强抗立枯丝核菌	[46]
StERF94	马铃薯	马铃薯	正调控	增强茄枯萎病的抗性	[27]