RNAi与转Bt基因技术协同抗虫研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2021-0244

摘要/Abstract

摘要：

转基因抗虫作物降低了昆虫的危害并减少了农药的使用,但是转Bt（Bacillus thuringiensis）基因抗虫作物的大面积连续种植使得靶标害虫对转Bt基因植物产生抗性进化,导致其长期抗虫效果受到挑战。为延缓靶标害虫对Bt蛋白的抗性进化,提高转基因作物的抗虫能力,RNA干扰（RNA interference,RNAi）技术与转Bt基因技术协同应用于农业害虫防治。本文综述了RNAi技术应用于农业害虫防控的进展,以及与转基因抗虫技术联合防治农业害虫的研究进展,分析其面临的挑战和技术难题,以期支撑RNAi与转基因技术发展成一种绿色环保和可持续发展的害虫综合防控技术。

关键词: 双链RNA, RNA干扰, 转基因作物, 害虫防治

Abstract:

The cultivation of transgenic insect-resistance crops has decreased the damage of insects on plants and the application of chemical pesticides in the field. However,the long-term continuous cultivation of Bt-transgenic crops worldwide has increased the resistance evolution of target insects to Bt-transgenic plants in the field,leading its long-term insect resistance to be in challenge. To delay the resistance evolution of target insects to Bt proteins and to improve the insect-resistance of transgenic crops,RNAi and Bt-transgenic biotechnologies have been synergistically applied to control agricultural pests. Here,we review the progresses on RNAi technology applied in agricultural pest-control and on the synergistic application of Bt-transgene technology in agricultural pest-control,and discuss the challenges and technological difficulties,aiming to promote the combined application of RNAi and transgenic technologies to be environment-friendly and sustainable in controlling agricultural pests.

Key words: dsRNA, RNA interference, genetically modified crops, pest control

邓普荣, 刘勇波. RNAi与转Bt基因技术协同抗虫研究进展[J]. 生物技术通报, 2021, 37(10): 216-224.

DENG Pu-rong, LIU Yong-bo. Review on the Synergistic Insect-resistant Application of RNAi and Bt-transgenic Technologies[J]. Biotechnology Bulletin, 2021, 37(10): 216-224.

图/表 2

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物种 Species	目标基因 Target gene	Bt蛋白基因 Bt gene	联合作效率 Synergistic efficiency	参考文献Reference
马铃薯甲虫 Leptinotarsa decemlineata	prohibitin-1	Cry3Aa	死亡 Mortality	[56]
海灰翅夜蛾 Spodoptera littoralis	Sl102	Cry1Ca	免疫力下降 Down-regulation of immunocompetence	[57]
亚洲玉米螟 Ostrinia furnacalis	chymotrypsin-like genes	Cry1Ab	死亡率显著升高 Significantly increased mortalities	[60]
甜菜夜蛾 Spodoptera exigua	CHS-B	Cry1Ac 和Cry1Ca	死亡 Mortality	[61]
棉铃虫 Helicoverpa armigera	JHAMT和JHBP	Cry1Ac	死亡 Mortality	[6]
棉铃虫 Helicoverpa armigera	v-ATPase-A	Cry1Ac	体重抑制率显著升高,对Cry1Ac更敏感 Higher weight inhibition,more sensitive to Cry1Ac	[62]

物种 Species	目标基因 Target gene	Bt蛋白基因 Bt gene	联合作效率 Synergistic efficiency	参考文献Reference
马铃薯甲虫 Leptinotarsa decemlineata	prohibitin-1	Cry3Aa	死亡 Mortality	[56]
海灰翅夜蛾 Spodoptera littoralis	Sl102	Cry1Ca	免疫力下降 Down-regulation of immunocompetence	[57]
亚洲玉米螟 Ostrinia furnacalis	chymotrypsin-like genes	Cry1Ab	死亡率显著升高 Significantly increased mortalities	[60]
甜菜夜蛾 Spodoptera exigua	CHS-B	Cry1Ac 和Cry1Ca	死亡 Mortality	[61]
棉铃虫 Helicoverpa armigera	JHAMT和JHBP	Cry1Ac	死亡 Mortality	[6]
棉铃虫 Helicoverpa armigera	v-ATPase-A	Cry1Ac	体重抑制率显著升高,对Cry1Ac更敏感 Higher weight inhibition,more sensitive to Cry1Ac	[62]

干扰途径 RNAi approach	植物 Plant	昆虫 Insect	目标基因 Target gene	参考文献 Reference
宿主诱导的基因沉默 HIGS	玉米和大豆 Zea mays and Glycine max	绿盲蝽 Apolygus lucorum	V-ATPase-E	[39]
	本氏烟 Nicotiana benthamiana	桃蚜 Myzus persicae	MpC002 and Rack-1	[36]
	拟南芥 Arabidopsis thaliana	桃蚜 Myzus persicae	MySP	[44]
	烟草 Nicotiana tabacum	烟粉虱 Bemisia tabaci	acetylcholinesterase（AChE） ecdysone receptor（EcR）	[37]
	小麦 Triticum aestivum	麦长管蚜 Sitobion avenae	zinc finger（SaZFP）	[40]
	马铃薯 Solanum tuberosum	马铃薯甲虫 Leptinotarsa decemlineata	EcR	[38]
	拟南芥 Arabidopsis thaliana 烟草 Nicotiana tabacum	棉铃虫 Helicoverpa armigera	CYP6AE14	[41]
	拟南芥 Arabidopsis thaliana	棉铃虫 Helicoverpa armigera	mitochondrial complex I（NDUFV2）	[19]
饲喂法 Dietary feeding		豌豆蚜 Acyrthosiphon pisum	ApAQP1	[76]
显微注射法 Microinjection		黄曲条跳甲 Phyllotreta striolata	odorant receptor（PsOr1）	[77]
		德国小蠊 Blattella germanica	RXR	[22]
微生物介导 Bacterial expression and oral delivery		粘虫 Mythimna separata	MseChi	[15]
		亚洲玉米螟 Ostrinia furnacalis	Glutathione-S-Transferase	[78]

干扰途径 RNAi approach	植物 Plant	昆虫 Insect	目标基因 Target gene	参考文献 Reference
宿主诱导的基因沉默 HIGS	玉米和大豆 Zea mays and Glycine max	绿盲蝽 Apolygus lucorum	V-ATPase-E	[39]
	本氏烟 Nicotiana benthamiana	桃蚜 Myzus persicae	MpC002 and Rack-1	[36]
	拟南芥 Arabidopsis thaliana	桃蚜 Myzus persicae	MySP	[44]
	烟草 Nicotiana tabacum	烟粉虱 Bemisia tabaci	acetylcholinesterase（AChE） ecdysone receptor（EcR）	[37]
	小麦 Triticum aestivum	麦长管蚜 Sitobion avenae	zinc finger（SaZFP）	[40]
	马铃薯 Solanum tuberosum	马铃薯甲虫 Leptinotarsa decemlineata	EcR	[38]
	拟南芥 Arabidopsis thaliana 烟草 Nicotiana tabacum	棉铃虫 Helicoverpa armigera	CYP6AE14	[41]
	拟南芥 Arabidopsis thaliana	棉铃虫 Helicoverpa armigera	mitochondrial complex I（NDUFV2）	[19]
饲喂法 Dietary feeding		豌豆蚜 Acyrthosiphon pisum	ApAQP1	[76]
显微注射法 Microinjection		黄曲条跳甲 Phyllotreta striolata	odorant receptor（PsOr1）	[77]
		德国小蠊 Blattella germanica	RXR	[22]
微生物介导 Bacterial expression and oral delivery		粘虫 Mythimna separata	MseChi	[15]
		亚洲玉米螟 Ostrinia furnacalis	Glutathione-S-Transferase	[78]